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Vol. 64 No. 6: Advances in mechanism and treatment strategy of cancer

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miR-140-5p suppresses retinoblastoma cell proliferation, migration, and invasion by targeting CEMIP and CADM3

https://doi.org/10.14715/cmb/2018.64.6.8
Xiaolin Miao
Outpatient Department, Eye Hospital of Wenzhou Medical University, China
Zhen Wang
Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou medical College, China
Bingyu Chen
Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou medical College, China
Yiqi Chen
Outpatient Department, Eye Hospital of Wenzhou Medical University, China
Xi Wang
Key Laboratory of Digestive Pathophysiology of Zhejiang Province, Zhejiang Hospital of Traditional Chinese Medicine, Zhejiang Chinese Medical University, China
Luxi Jiang
Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou medical College, China
Shanshan Jiang
Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou medical College, China
Ke Hao
Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou medical College, China
Wei Zhang
Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou medical College, China

Corresponding Author(s) : Wei Zhang

weizhang2018@hotmail.com

Cellular and Molecular Biology, Vol. 64 No. 6: Advances in mechanism and treatment strategy of cancer

Abstract

Retinoblastoma (RB) is a childhood intraocular tumor, affecting millions of patients worldwide. MicroRNA-140-5p (miR-140-5p) was demonstrated to be involved in the tumorigenesis of various human cancers; however, its role in RB remains undetermined. In this study, quantitative real-time PCR (qRT-PCR) and Western blot assays were used to determine the expression levels of miR-140-5p, cell migration-inducing protein (CEMIP), and cell adhesion molecule 3 (CADM3) in RB tissues and cell-lines. The proliferation ability was detected by cell-counting kit 8 (CCK-8), Edu staining, and colony formation assay. The cell cycle and migration and invasion abilities were measured by flow cytometry, wound-healing assay and Transwell assays, respectively. The correlation between miR-140-5p and CEMIP/CADM3 were then confirmed by immunofluorescence (IF) and dual-luciferase reporter assays. The results showed that miR-140-5p expression was significantly decreased; however, CEMIP and CADM3 expression was increased in RB tissues and cells. Overexpression of miR-140-5p inhibited proliferation, migration, and invasion of RB cells. We also found that miR-140-5p inhibited CEMIP and CADM3 expressions in RB cells. In addition, we demonstrated that miR-140-5p might negatively regulate the transcriptional activities of CEMIP and CADM3 by targeting their 3'-UTR. Therefore, we suggested that miR-140-5p could be a potential therapeutic target for the treatment of RB through CEMIP and CADM3.

Keywords

MicroRNA-140-5p Retinoblastoma Cell migration inducing protein Cell adhesion molecule 3 Proliferation Migration and invasion.
Miao, X., Wang, Z., Chen, B., Chen, Y., Wang, X., Jiang, L., Jiang, S., Hao, K., & Zhang, W. (2018). miR-140-5p suppresses retinoblastoma cell proliferation, migration, and invasion by targeting CEMIP and CADM3. Cellular and Molecular Biology, 64(6), 42–47. https://doi.org/10.14715/cmb/2018.64.6.8
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Author Biographies

Xiaolin Miao, Outpatient Department, Eye Hospital of Wenzhou Medical University, China

Zhen Wang, Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou medical College, China

Bingyu Chen, Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou medical College, China

Yiqi Chen, Outpatient Department, Eye Hospital of Wenzhou Medical University, China

Xi Wang, Key Laboratory of Digestive Pathophysiology of Zhejiang Province, Zhejiang Hospital of Traditional Chinese Medicine, Zhejiang Chinese Medical University, China

Luxi Jiang, Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou medical College, China

Shanshan Jiang, Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou medical College, China

Wei Zhang, Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou medical College, China

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