Issue

Vol. 64 No. 6: Advances in mechanism and treatment strategy of cancer

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

miR-140-5p suppresses retinoblastoma cell proliferation, migration, and invasion by targeting CEMIP and CADM3

https://doi.org/10.14715/cmb/2018.64.6.8
Xiaolin Miao
Outpatient Department, Eye Hospital of Wenzhou Medical University, China
Zhen Wang
Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou medical College, China
Bingyu Chen
Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou medical College, China
Yiqi Chen
Outpatient Department, Eye Hospital of Wenzhou Medical University, China
Xi Wang
Key Laboratory of Digestive Pathophysiology of Zhejiang Province, Zhejiang Hospital of Traditional Chinese Medicine, Zhejiang Chinese Medical University, China
Luxi Jiang
Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou medical College, China
Shanshan Jiang
Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou medical College, China
Ke Hao
Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou medical College, China
Wei Zhang
Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou medical College, China

Corresponding Author(s) : Wei Zhang

weizhang2018@hotmail.com

Cellular and Molecular Biology, Vol. 64 No. 6: Advances in mechanism and treatment strategy of cancer

Abstract

Retinoblastoma (RB) is a childhood intraocular tumor, affecting millions of patients worldwide. MicroRNA-140-5p (miR-140-5p) was demonstrated to be involved in the tumorigenesis of various human cancers; however, its role in RB remains undetermined. In this study, quantitative real-time PCR (qRT-PCR) and Western blot assays were used to determine the expression levels of miR-140-5p, cell migration-inducing protein (CEMIP), and cell adhesion molecule 3 (CADM3) in RB tissues and cell-lines. The proliferation ability was detected by cell-counting kit 8 (CCK-8), Edu staining, and colony formation assay. The cell cycle and migration and invasion abilities were measured by flow cytometry, wound-healing assay and Transwell assays, respectively. The correlation between miR-140-5p and CEMIP/CADM3 were then confirmed by immunofluorescence (IF) and dual-luciferase reporter assays. The results showed that miR-140-5p expression was significantly decreased; however, CEMIP and CADM3 expression was increased in RB tissues and cells. Overexpression of miR-140-5p inhibited proliferation, migration, and invasion of RB cells. We also found that miR-140-5p inhibited CEMIP and CADM3 expressions in RB cells. In addition, we demonstrated that miR-140-5p might negatively regulate the transcriptional activities of CEMIP and CADM3 by targeting their 3'-UTR. Therefore, we suggested that miR-140-5p could be a potential therapeutic target for the treatment of RB through CEMIP and CADM3.

Keywords

MicroRNA-140-5p Retinoblastoma Cell migration inducing protein Cell adhesion molecule 3 Proliferation Migration and invasion.
Miao, X., Wang, Z., Chen, B., Chen, Y., Wang, X., Jiang, L., Jiang, S., Hao, K., & Zhang, W. (2018). miR-140-5p suppresses retinoblastoma cell proliferation, migration, and invasion by targeting CEMIP and CADM3. Cellular and Molecular Biology, 64(6), 42–47. https://doi.org/10.14715/cmb/2018.64.6.8
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. Milenkovic D, Jude B, Morand C. miRNA as molecular target of polyphenols underlying their biological effects. Free Radic Biol Med. Sep 2013; 64: 40-51.
  2. Petrovic N, Ergun S. miRNAs as Potential Treatment Targets and Treatment Options in Cancer. Mol Diagn Ther. Jan 15 2018.
  3. Gurtan AM, Sharp PA. The role of miRNAs in regulating gene expression networks. J Mol Biol. Oct 9 2013; 425(19): 3582-3600.
  4. Ullah S, John P, Bhatti A. MicroRNAs with a role in gene regulation and in human diseases. Mol Biol Rep. Jan 2014; 41(1): 225-232.
  5. Romero-Cordoba SL, Salido-Guadarrama I, Rodriguez-Dorantes M, Hidalgo-Miranda A. miRNA biogenesis: biological impact in the development of cancer. Cancer Biol Ther. 2014; 15(11): 1444-1455.
  6. Xie X, Tan W, Chen B et al. Preoperative prediction nomogram based on primary tumor miRNAs signature and clinical-related features for axillary lymph node metastasis in early-stage invasive breast cancer. Int J Cancer. Dec 11 2017.
  7. Pereira CM, Sehnem D, da Fonseca EO et al. miRNAs: Important Targets for Oral Cancer Pain Research. Biomed Res Int. 2017; 2017: 4043516.
  8. Slattery ML, Pellatt AJ, Lee FY et al. Infrequently expressed miRNAs influence survival after diagnosis with colorectal cancer. Oncotarget. Oct 13 2017; 8(48): 83845-83859.
  9. Bertoli G, Cava C, Castiglioni I. MicroRNAs: New Biomarkers for Diagnosis, Prognosis, Therapy Prediction and Therapeutic Tools for Breast Cancer. Theranostics. 2015; 5(10): 1122-1143.
  10. Xie C, Han Y, Liu Y, Han L, Liu J. miRNA-124 down-regulates SOX8 expression and suppresses cell proliferation in non-small cell lung cancer. Int J Clin Exp Pathol. 2014; 7(10): 6534-6542.
  11. Huang WC, Chan SH, Jang TH et al. miRNA-491-5p and GIT1 serve as modulators and biomarkers for oral squamous cell carcinoma invasion and metastasis. Cancer Res. Feb 1 2014; 74(3): 751-764.
  12. Macha MA, Seshacharyulu P, Krishn SR et al. MicroRNAs (miRNAs) as biomarker(s) for prognosis and diagnosis of gastrointestinal (GI) cancers. Curr Pharm Des. 2014; 20(33): 5287-5297.
  13. Zhang K, Zhang Y, Liu C, Xiong Y, Zhang J. MicroRNAs in the diagnosis and prognosis of breast cancer and their therapeutic potential (review). Int J Oncol. Sep 2014; 45(3): 950-958.
  14. Nassar FJ, Nasr R, Talhouk R. MicroRNAs as biomarkers for early breast cancer diagnosis, prognosis and therapy prediction. Pharmacol Ther. Apr 2017; 172: 34-49.
  15. Rodriguez-Galindo C, Orbach DB, VanderVeen D. Retinoblastoma. Pediatr Clin North Am. Feb 2015; 62(1): 201-223.
  16. Mendoza PR, Grossniklaus HE. The Biology of Retinoblastoma. Prog Mol Biol Transl Sci. 2015; 134: 503-516.
  17. Lumbroso-Le Rouic L, Savignoni A, Levy-Gabriel C et al. Treatment of retinoblastoma: The Institut Curie experience on a series of 730 patients (1995 to 2009). J Fr Ophtalmol. Jun 2015; 38(6): 535-541.
  18. Jehanne M, Brisse H, Gauthier-Villars M, Lumbroso-le Rouic L, Freneaux P, Aerts I. [Retinoblastoma: recent advances]. Bull Cancer. Apr 2014; 101(4): 380-387.
  19. Mouw KW, Sethi RV, Yeap BY et al. Proton radiation therapy for the treatment of retinoblastoma. Int J Radiat Oncol Biol Phys. Nov 15 2014; 90(4): 863-869.
  20. Montoya V, Fan H, Bryar PJ et al. Novel miRNA-31 and miRNA-200a-Mediated Regulation of Retinoblastoma Proliferation. PLoS One. 2015; 10(9): e0138366.
  21. Yang Y, Mei Q. miRNA signature identification of retinoblastoma and the correlations between differentially expressed miRNAs during retinoblastoma progression. Mol Vis. 2015; 21: 1307-1317.
  22. Gullu G, Peker I, Haholu A et al. Clinical significance of miR-140-5p and miR-193b expression in patients with breast cancer and relationship to IGFBP5. Genet Mol Biol. Mar 2015; 38(1): 21-29.
  23. Shin VY, Ng EK, Chan VW, Kwong A, Chu KM. A three-miRNA signature as promising non-invasive diagnostic marker for gastric cancer. Mol Cancer. Nov 25 2015; 14: 202.
  24. Flamini V, Jiang WG, Cui Y. Therapeutic Role of MiR-140-5p for the Treatment of Non-small Cell Lung Cancer. Anticancer Res. Aug 2017; 37(8): 4319-4327.
  25. McEvoy J, Nagahawatte P, Finkelstein D et al. RB1 gene inactivation by chromothripsis in human retinoblastoma. Oncotarget. Jan 30 2014; 5(2): 438-450.
  26. Mehta M, Sethi S, Pushker N et al. Retinoblastoma. Singapore Med J. Feb 2012; 53(2): 128-135; quiz 136.
  27. Wang J, Wang X, Li Z, Liu H, Teng Y. MicroRNA-183 suppresses retinoblastoma cell growth, invasion and migration by targeting LRP6. FEBS J. Mar 2014; 281(5): 1355-1365.
  28. Sun Z, Zhang A, Jiang T, Du Z, Che C, Wang F. MiR-145 suppressed human retinoblastoma cell proliferation and invasion by targeting ADAM19. Int J Clin Exp Pathol. 2015; 8(11): 14521-14527.
  29. Conkrite K, Sundby M, Mukai S et al. miR-17~92 cooperates with RB pathway mutations to promote retinoblastoma. Genes Dev. Aug 15 2011; 25(16): 1734-1745.
  30. Fink SP, Myeroff LL, Kariv R et al. Induction of KIAA1199/CEMIP is associated with colon cancer phenotype and poor patient survival. Oncotarget. Oct 13 2015; 6(31): 30500-30515.
  31. Li L, Yan LH, Manoj S, Li Y, Lu L. Central Role of CEMIP in Tumorigenesis and Its Potential as Therapeutic Target. J Cancer. 2017; 8(12): 2238-2246.
  32. Galamb O, Kalmar A, Bartak BK et al. Aging related methylation influences the gene expression of key control genes in colorectal cancer and adenoma. World J Gastroenterol. Dec 21 2016; 22(47): 10325-10340.
  33. Gombodorj N, Yokobori T, Yoshiyama S et al. Inhibition of Ubiquitin-conjugating Enzyme E2 May Activate the Degradation of Hypoxia-inducible Factors and, thus, Overcome Cellular Resistance to Radiation in Colorectal Cancer. Anticancer Res. May 2017; 37(5): 2425-2436.
  34. Liang G, Fang X, Yang Y, Song Y. Silencing of CEMIP suppresses Wnt/beta-catenin/Snail signaling transduction and inhibits EMT program of colorectal cancer cells. Acta Histochem. Jan 2018; 120(1): 56-63.
  35. Opilka MN, Lorenc Z, Starzewska M, Lorenc J, Rajs A. Cell adhesion molecules in terms of carcinogenesis. Pol Przegl Chir. Mar 2014; 86(3): 151-157.
  36. Ivanov AN, Norkin IA, Puchin'ian DM, Shirokov V, Zhdanova O. [Endothelial cell adhesion molecules]. Usp Fiziol Nauk. Oct-Dec 2014; 45(4): 34-49.
  37. Samanta D, Almo SC. Nectin family of cell-adhesion molecules: structural and molecular aspects of function and specificity. Cell Mol Life Sci. Feb 2015; 72(4): 645-658.
Read More

Author Biographies

Xiaolin Miao, Outpatient Department, Eye Hospital of Wenzhou Medical University, China

Zhen Wang, Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou medical College, China

Bingyu Chen, Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou medical College, China

Yiqi Chen, Outpatient Department, Eye Hospital of Wenzhou Medical University, China

Xi Wang, Key Laboratory of Digestive Pathophysiology of Zhejiang Province, Zhejiang Hospital of Traditional Chinese Medicine, Zhejiang Chinese Medical University, China

Luxi Jiang, Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou medical College, China

Shanshan Jiang, Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou medical College, China

Wei Zhang, Research Center of Blood Transfusion Medicine, Education Ministry Key Laboratory of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou medical College, China

Crossref
Scopus
Google Scholar
Europe PMC
Download this PDF file
PDF
Statistic
Read Counter : 934 Download : 473

Most read articles by the same author(s)