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Copyright (c) 2022 Pengfei Xie, Zhen Wang, Xia Chen, Ying Han, Mei Yang, Zhuang Ye
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.Regulations of LINC0196/miR-584-5p/miR-34a-5p/TRIM59 on Progression of Pediatric Neuroblastoma
Corresponding Author(s) : Zhuang Ye
Cellular and Molecular Biology,
Vol. 68 No. 6: Issue 6
Abstract
This work was to study the regulatory mechanism of large intergenic non-coding RNA 0196 (LINC0196), miR-584-5p, miR-34a-5p, and tripartite motif 59 (TRIM59) on neuroblastoma. The interaction among the four was analyzed to provide a research basis for the clinical treatment of neuroblastoma at the molecular level. The human neuroblastoma SK-N-SH cells were collected and cultured. According to the transfection methods, the cells were divided into control group (without any treatment), si-LINC0196 group (si-LINC0196 transfection), si-LINC0196-NC group (si-LINC0196 vector transfection), miR-584-5p group (miR-584-5p mimic transfection), miR-584-5p-NC group (miR-584-5p inhibitor transfection), miR-34a-5p group (miR-34a-5p mimic transfection), and miR-34a-5p-NC group (miR-34a-5p inhibitor transfection). The proliferation, migration, and apoptosis of SK-N-SH cells in each group were compared. The effects of LINC0196, miR-584-5p, miR-34a-5p, and TRIM59 were evaluated. The expressions of LINC0196 and TRIM59 in SK-N-SH cells in si-LINC0196, miR-584-5p, and miR-34a-5p groups were up-regulated. miR-584-5p and miR-34a-5p in si-LINC0196-NC, miR-584-5p-NC, and miR-34a-5p-NC groups decreased significantly (P < 0.05). The proliferation rate, migration rate, and invasiveness of SK-N-SH cells in miR-584-5p and miR-34a-5p groups were lower than those in si-LINC0196-NC, miR-584-5p-NC, and miR-34a-5p-NC groups, while the apoptosis rate increased (P < 0.05). After miR-584-5p and miR-34a-5p transfections, the relative activities of WT-LINC0196 and WT-TRIM59 dual luciferase were greatly inhibited (P < 0.05). LINC0196 could regulate TRIM59 by regulating miR-584-5p and miR-34a-5p, thereby indirectly regulating cell proliferation, apoptosis, migration, and invasion of SK-N-SH cells.
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