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Copyright (c) 2022 Jiabo Yuan, Wei Zhang
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.Expression and significance of TGF-β1 in Infant Asthma Model
Corresponding Author(s) : Wei Zhang
Cellular and Molecular Biology,
Vol. 68 No. 7: Issue 7
Abstract
This article aimed to study the expression and role of TGF-β1 in young rat asthma. The experimental method in this paper is to replicate the asthma model by aerosolization of egg protein. The young rats with asthma were sacrificed after 2, 4 and 8 weeks. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the mRNA of TGF-β1 and α-SMA. The expression level was detected by the immunohistochemical staining method to detect the protein expression of TGF-β1, α-SMA, Smad3, Smad4 and Smad7 in bronchopulmonary tissues. At the same time, the image analysis method was used to measure the airway morphological parameters of young rats. The experimental results showed that the expression of TGF-β1 in the lung tissue of young rats in the asthma group was increased after two weeks of the challenge. Compared with the control group, the difference was significant (P<0.05); the expression of TGF-β1, α-SMA and mRNA in the 4-week group was significantly higher, compared with the control group and the 2-week challenge group, the difference was significant (P<0.05). Therefore, the change of asthma airway remodeling is a dynamic process. The TGF-β1 pathway presents a dynamic process in the airway remodeling model of young asthmatic rats. Among them, TGF-β1 induces the division and proliferation of airway fibroblasts and promotes fibroblasts. The cells transform into myofibroblasts and increase the synthesis of fibronectin and collagen, suggesting that it plays an important role in asthma inflammation and pulmonary fibrosis.
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