Issue

Vol. 63 No. 8: Issue 8

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

Investigation of the irisin, preptin and adropin levels in the blood serum of Alburnus tarichi

https://doi.org/10.14715/cmb/2017.63.8.20
F. Caf
Technical Science Vocational High School, Bingol University, Bingöl, Turkey
S. Algul
Faculty of Medicine, Department of Physiology, YuzuncuYil University, Van, Turkey
M. Koyun
Sciences and Arts Faculty, Department of Biology, Bingol University, Bingöl, Turkey

Corresponding Author(s) : F. Caf

f.baydas23@hotmail.com

Cellular and Molecular Biology, Vol. 63 No. 8: Issue 8

Abstract

Irisin, preptin and adropin are three newly discovered peptides that play critical roles in regulating energy homeostasis in various vertebrates.The purposes of this study were to measure the serum concentrations of preptin, adropin and irisin in the Alburnus tarichi and to investigate the relationship of these peptides to the weight, gender and length of this the fish, which will provide useful information for future biotechnology purposes aimed at improvements in aquaculture production. This study used 12 adult A. tarichi (6 female and 6 male) obtained from Van Lake (Van, Turkey). The serum irisin, preptin and adropin levels were measured using a commercially available enzyme-linked immunosorbent assay (ELISA) kit to determine correlations between the levels of these three hormones and fish body weight and length.  No statistically significant correlations were detected between the serum irisin, adropin and preptin levels and A. tarichi body weight (p = 0.921, r = -0.031; p = 0.08, r = 0.519; p = 0.461, r = -0.235, respectively) or length (p = 0.901, r = -0.040; p = 0.105, r = 0.490; p = 0.236, r = -0.369, respectively).Thus, serum levels of these hormones are independent of fish gender, body weight and length.

Keywords

A. tarichi Irisin Preptin Adropin Metabolic hormone.
Caf, F., Algul, S., & Koyun, M. (2017). Investigation of the irisin, preptin and adropin levels in the blood serum of Alburnus tarichi. Cellular and Molecular Biology, 63(8), 95–99. https://doi.org/10.14715/cmb/2017.63.8.20
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. Lian A, Wu K, LiuT, Jiang N, Jiang Q. Adropin induction of lipoprotein lipase expression in tilapia hepatocytes. J Mol Endocrinol 2016; 56: 11–22.
  2. Aydın S. Three new players in energy regulation: preptin, adropin and irisin. Peptides 2014; 56: 94-110.
  3. Vaughan RA, Gannon NP, Barberena MA, Garcia-Smith R, Bisoffi M, Mermier CM, et al. Characterization of the metabolic effects of irisin on skeletal muscle in vitro. Diabetes Obes Metab 2014; 16: 711–718.
  4. Piya MK, Harte AL, Sivakumar K, Tripathi G, Voyias PD, James S, Sabico S, Al-Daghri NM, Saravanan P, Barber TM, Kumar S, Vatish M, McTernan PG. The identification of irisin in human cerebrospinal fluid: influence of adiposity, metabolic markers and gestational diabetes. Am J Physiol Endocrinol Metab 2014; 306(5): E512-E518.
  5. Butler AA, Tam CS, Stanhope KL, Wolfe BM, Ali MR, O'Keeffe M, et al. Low circulating adropin concentrations with obesity and aging correlate with risk factors for metabolic disease and increase after gastric bypass surgery in humans. J Clin Endocrinol Metab 2012; 97:3783–91.
  6. Kumar KG, Trevaskis JL, Lam DD. Identification of adropin as a secreted factor linking dietary macronu-trient intake with energy homeostasis and lipid metabolism. Cell Metab 2008; 8: 468–81.
  7. Ganesh Kumar, K, Zhang J, Gao S, Rossi J, McGuinness OP, Halem, HH, et al. Adropin deficiency is associated with increased adiposity and insulin resistance. Obesity (Silver Spring) 2012; 20: 1394–402.
  8. Wu L, Fang J, Chen L, Zhao Z, Luo Y, Lin C, et al. Low serum adropin is associated with coronary atherosclerosis in type 2 diabetic and non-diabetic patients. Clin Chem Lab Med 2013; 9: 1–8.
  9. Topuz M, Celik A, Aslantas T, Demir AK, Aydin S. Plasma adropin levels predict endothelial dysfunction like flow-mediated dilatation in patients with type 2 diabetes mellitus. J Investig Med 2013; 61:1161–1164.
  10. Buchanan CM, Phillips AR, Cooper GJ. 2001. Preptin derived from proinsulin-like growth factor II (proIGF-II) is secreted from pancreatic islet beta-cells and enhances insulin secretion". Biochem J 360, 431–439.
  11. Liu YS, Lu Y, Liu W. Connective tissue growth factor is a downstream mediator for preptin-induced proliferation and differentiation in human osteoblasts. Amino Acids 2010; 38, 763–769.
  12. Cooper GJ, Willis AC, Clark A. Purification and characterization of a peptide from amyloid-rich pancreases of type 2 diabetic patients. Natl Acad Sci USA 1987; 84: 8628–8632.
  13. Boström P, Wu J, Jedrychowski MP, Korde A, Ye L, Lo JC, et al. A PGC1-α dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature 2012; 481(7382): 463-8.
  14. Sarı M. Threatened fishes of the world: Chalcalburnus tarichi (Pallas 1811) (Cyprinidae) living in the highly alkaline Lake Van, Turkey. Environ Biol Fish 2008; 81: 21-23.
  15. Kempe S, Kazmierczak J, Landmann G, Konuk T, Reimer A, Lipp A. Largest known microbialites discovered in Lake Van, Turkey. Nature 1991; 349: 605–608.
  16. Arabaci M, Sari M. Induction of ovulation in endemic pearl mullet (Chalcalburnus tarichi), living in the highly alkaline Lake Van, using GnRHa([DSer(tBu)6, Pro9-Net]-GnRH) combined with haloperidol. Aquaculture 2004; 238, 529–535.
  17. Yang GY, Li L, Chen WW, Liu H, Boden G, Li K. Circulating preptin levels in normal, impaired glucose tolerance, and type 2 diabetic subjects. Ann Med 2009; 41: 52–56.
  18. Elp M, Åžen F Atıcı, AA. ć°nci Kefalinin (Alburnus tarichi (Guldenstaedtii, 1814)) Van Gölü Havzası Su Kaynaklarındaki Yayılım Bölgeleri. Yyuj J Agr 2014; 24(3): 228-232.
  19. Kono T, Kitao Y, Sonoda K, Nomoto R, Mekata T, Sakai M. Identfication and expression analysis of ghrelin gene in common carp, Cyprinus carpio Fisheries Sci 2008; 74: 603–612.
  20. Köprücü S, Algül S. Comparatively examining of the apelin-13 levels in the Capoeta trutta (Heckel, 1843) and Cyprinus carpio (Linnaeus, 1758). J Anim Physiol Anim Nutr (Berl) 2015a; 99: 210-214.
  21. Gonzalez R, Kerbel B, Chun A, Unniappan S. Molecular, cellular and physiological evidences for the anorexigenic actions of nesfatin-1 in goldfish. PLoS ONE 2010; 5: 15201.
  22. Lin F, Zhou C, Chen H, Wu H, Xin Z, Liu J, Gao Y, Yuan D, Wang T, Wei R, Chen D, Yang S, Wang Y, Pu Y, Li Z. Molecular characterization, tissue distribution and feeding related changes of NUCB2A/nesfatin-1 in Ya-fish (Schizothorax prenanti). Gene 2014; 536: 238-246.
  23. Hatef A, Shajan S, Unniappan S. Nutrient status modulates the expression of nesfatin-1 encoding nucleobindin 2A and 2B mRNAs in zebrafish gut, liver and brain. Gen. Comp. Endocrinol. 2015; 215: 51-60.
  24. Huising MO, Geven EJ, Kruiswijk CP, Nabuurs SB, Stolte EH, Spanings FA, Verburg-van Kemenade BM, Flik G. Increased leptin expression in common Carp (Cyprinus carpio) after food intake but not after fasting or feeding to satiation. Endocrinology 2006; 147: 5786-5797.
  25. Köprücü S, Algül S. Investigation of the leptin levels in the blood serum of Cyprinus carpio (Linnaeus, 1758) and Capoeta trutta (Heckel, 1843). J Anim Physiol Anim Nutr (Berl) 2015b; 99: 430-435.
  26. Huh JY, Dincer F, Mesfum E, Mantzoros CS. Irisin stimulates muscle growthrelated genes and regulates adipocyte differentiation and metabolism in humans. Int J Obes 2014; 38: 1538–1544.
  27. Li X, Fang W, Hu Y, Wang Y, Li J. Characterization of fibronectin type III
  28. domain-containing protein 5(FNDC5) gene in chickens: cloning, tissue
  29. expression, and regulation of its expression in the muscle by fasting and cold
  30. exposure. Gene 2015; 570: 221-229.
  31. Varela-Rodriguez BM, Pena-Bello L, Juiz-Valina P, Vidal-Bretal B, Cordido F, Sangiao-Alvarellos S. FNDC5 expression and circulating irisin levels are modified by diet and hormonal conditions in hypothalamus, adipose tissue and muscle. Sci Rep 2016; 6: 29898.
  32. Wu F, Song H, Zhang Y, Zhang Y, Mu Q, Jiang M, Wang F, Zhang W, Li L, Li H, Wang Y, Zhang M, Li S, Yang L, Meng Y, Tang D. Irisin Induces Angiogenesis in Human Umbilical Vein Endothelial Cells In Vitro and in Zebrafish Embryos In Vivo via Activation of the ERK Signaling Pathway. PLoS One 2015; 10(8): e0134662.
  33. Lian A, Li X, Jiang Q. Irisin inhibition of growth hormone secretion in cultured tilapia pituitary cells. Mol Cell Endocrinol 2017; 439: 395-406.
  34. Swick AG, Orena S, O'Connor A. Irisin levels correlate with energy expenditure in a subgroup of humans with energy expenditure greater than predicted by fat free mass. Metabolism 2013; 62: 1070–1073.
  35. Choi YK, Kim MK, Bae KH, Seo HA, Jeong JY, Lee WK, et al. Serum irisin levels in new-onset type 2 diabetes. Diabetes Res. Clin. Pract 2013; 100: 96–101.
  36. Stengel A, Hofmann T, Goebel-Stengel M, Elbelt U, Kobelt P, Klapp BF. Circulating levels of irisin in patients with anorexia nervosa and different stages of obesity”Correlation with body mass index. Peptides 2013; 39: 125–130.
  37. ć°nci A. íœnübol Aypak S. ć°risin ve Metabolik Etkileri. Turkiye Klinikleri J Endocrin 2016; 11(1):15-21.
  38. Echtay KS. Mitochondrial uncoupling proteins-what is their physiological role? Free Radic Biol Med 2007; 43: 1351-71.
  39. Carroll AM, Haines LR, Pearson TW, Brennan C, Breen EP, Porter RK. Immunodetection of UCP1 in rat thymocytes. Biochem. Soc. Trans. 2004; 32: 1066-1067.
  40. Lian W, Gu X, Qin Y, Zheng X. Elevated Plasma Levels of Adropin in Heart Failure Patients. Intern Med 2011; 50: 1523-1527.
  41. Mierzwicka A, Bolanowski M. New peptides players in metabolic disorders. Postepy Hig Med Dosw 2016; 70: 881-886.
Read More
Author biographies is not available.
Crossref
Scopus
Google Scholar
Europe PMC
Download this PDF file
PDF
Statistic
Read Counter : 1020 Download : 348