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Vol. 64 No. 11: Issue 11

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Effect of hesperetin on inflammatory and oxidative status in trinitrobenzene sulfonic acid-induced experimental colitis model

https://doi.org/10.14715/cmb/2018.64.11.11
Fatin Rustu Polat
Tekirdag Namik Kemal University Medical Faculty, Department of Surgery, Tekirdag, Turkey
Ihsan Karaboga
Tekirdag Namik Kemal University, School of Health, Tekirdag, Turkey
Muhammed Semih Polat
Istanbul University, Engineering Faculty, Istanbul, Turkey
Zeynep Erboga
Tekirdag Namik Kemal University, Medical Faculty, Department of Histology and Embryology, Tekirdag, Turkey
Ahsen Yilmaz
Tekirdag Namik Kemal University, Medical Faculty, Department of Histology and Embryology, Tekirdag, Turkey
Savas Güzel
Tekirdag Namik Kemal University, Medical Faculty, Department of Medical Biochemistry, Tekirdag, Turkey

Corresponding Author(s) : Fatin Rustu Polat

polat22@hotmail.com

Cellular and Molecular Biology, Vol. 64 No. 11: Issue 11

Abstract

In our study, the effect of hesperetin on inflammatory and oxidative status in trinitrobenzene sulfonic acid (TNBS)-induced experimental colitis model was investigated through different methods. Eighteen Wistar albino male rats were divided in to three groups: Group I (Control, n = 8; 1 ml physiological saline), Group II (Colitis, n = 8; 1 ml TNBS), Group III (Hesperetin, n = 8; 1 ml TNBS and 100 mg/kg hesperetin). Macroscopic and microscopic scores were calculated to determine the damage to the colon at the end of the experiment. Serum tumor necrosis factor-α (TNF-α) and tissue interleukin-6 (IL-6) levels were determined using the ELISA method. Myeloperoxidase (MPO), superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) levels were investigated spectrophotometrically. The TUNEL method was used for the detection of apoptotic cells in the colon tissue. Inducible nitric oxide synthase (iNOS) and nuclear factor-kappa-B (NF-ĸβ) expression in the colon were determined immunohistochemically. Hesperetin administration has shown to significantly reduce levels of MPO, MDA, and proinflammatory agents (TNF-α, IL-6, and NF-ĸβ). It has also been proven to inhibit mucosal apoptosis. This study indicates that hesperetin is protective against TNBS-induced colitis model via antiinflammatory, antioxidant and antiapoptotic effects.

Keywords

Hesperetin TBNS-induced colitis NF-ĸβ iNOS IL-6 MPO.
Polat, F. R., Karaboga, I., Polat, M. S., Erboga, Z., Yilmaz, A., & Güzel, S. (2018). Effect of hesperetin on inflammatory and oxidative status in trinitrobenzene sulfonic acid-induced experimental colitis model. Cellular and Molecular Biology, 64(11), 58–65. https://doi.org/10.14715/cmb/2018.64.11.11
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