Protective effect of gel form of gastric gavage applicated aloe vera on ischemia reperfusion injury in renal and lung tissue

Hasan Sahin; Ali Umit Yener; Ihsan Karaboga; Muserref Hilal Sehitoglu; Tugba Dogu; Hatice Betul Altinisik; Ugur Altinisik; Tuncer Simsek

doi:10.14715/10.14715/cmb/2017.63.12.9

Issue

Vol. 63 No. 12: Issue 12

Issue Published : December 19, 2017

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Protective effect of gel form of gastric gavage applicated aloe vera on ischemia reperfusion injury in renal and lung tissue

https://doi.org/10.14715/10.14715/cmb/2017.63.12.9

Hasan Sahin

Department of Anesthesiology and Reanimation, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey

Ali Umit Yener

Department of Cardiovascular Surgery, Medical Park Hospital, Ordu, Turkey

Ihsan Karaboga

School of Health, Namık Kemal University, Tekirdag, Turkey

Muserref Hilal Sehitoglu

Department of Biochemistry, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey

Tugba Dogu

Anesthesiologist, Faculty of Medicine, University of Wisconsin-Madison, Wisconsin, USA

Hatice Betul Altinisik

Department of Anesthesiology and Reanimation, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey

Ugur Altinisik

Anesthesiologist, í‡anakkale, Turkey

Tuncer Simsek

Department of Anesthesiology and Reanimation, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey

Corresponding Author(s) : Hasan Sahin

drsahin17@gmail.com.tr

Cellular and Molecular Biology, Vol. 63 No. 12: Issue 12
Article Published : December 30, 2017

Abstract

The aloe vera plant has become increasingly popular in recent years. This study aimed to research the effect of aloe vera to prevent renal and lung tissue damage in an experimental ischemia–reperfusion (I/R) injury model. The study included 21 male Wistar Albino rats, which were categorized into control group, n = 7 (no procedures), Sham group n = 7 (I/R); and aloe vera therapy group, n = 7 (aloe vera and I/R). Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and malondialdehyde (MDA) were evaluated from lung and kidney tissues for biochemical investigations. As histopathological, hematoxylin and eosin and anti-iNOS were also examined. In biochemical investigations, SOD, CAT, and GPx levels of the Sham group were found to be lower compared with the other groups (P < 0.05). The aloe vera therapy group was not statistically different from control groups but significantly different compared with the Sham group. In the same way, the MDA levels of kidney and lung tissues were statistically significant in the aloe vera therapy group, compared to the Sham group. In the Sham group, the peribronchial and perialveolar edema were observed in lung parenchyma. Also, excess interstitial hemorrhage, leukocyte infiltration, and alveolar wall thickening were identified in ischemic groups. The histopathological changes were much lighter than in the aloe vera therapy group. In renal tissues, excess epithelial cell deterioration, tubular desqumination, and glomerular atrophy were observed in the Sham group. The histopathological changes were markedly reduced in the aloe vera therapy group. In the kidney and lung tissue, the level of iNOS activity in the Sham group was significantly higher than in the control and aloe vera therapy group. This study indicated that aloe vera is protective against oxidative damage formed by I/R in distant organs like the lungs and kidneys.

Keywords

Aloe vera I/R Ischemia and reperfusion Kidney Lung.

Sahin, H., Yener, A. U., Karaboga, I., Sehitoglu, M. H., Dogu, T., Altinisik, H. B., Altinisik, U., & Simsek, T. (2017). Protective effect of gel form of gastric gavage applicated aloe vera on ischemia reperfusion injury in renal and lung tissue. Cellular and Molecular Biology, 63(12), 34–39. https://doi.org/10.14715/10.14715/cmb/2017.63.12.9

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References

- Maxwell SR, Lip GY. Reperfusion injury: a review of the pathophysiology, clinical manifestations and therapeutic options. Int J Cardiol. 1997;58:95-117.
- Carden DL, Granger DN. Pathophysiology of ischaemia-reperfusion injury. J Pathol.
;190:255-266.
- Collard CD, Gelman S. Pathophysiology, clinical manifestations, and prevention of ischemia-reperfusion injury. Anesthesiology. 2001;94:1133-1138.
- Nakamura T, Vollmar B, Winning J, Ueda M, Menger MD, Schäfers HJ. Heparin and the nonanticoagulant N-acetyl heparin attenuate capillary no-reflow after normothermic ischemia of the lung. Ann Thorac Surg. 2001;72:1183-1188.
- Nastos C, Kalimeris K, Papoutsidakis N, et al. Global consequences of liver ischemia/reperfusion injury. Oxid Med Cell Longev. 2014;2014, Article ID 906965, 13p.
- Ghasemnejad-berenji M, Ghazi-Khansari M, Yazdani I, Saravi SSS, Nobakht M, Abdollahi A, Ansari JM, Ghasemnejad-berenji H, Pashapour S, and Dehpour AR. Rapamycin protects testes against germ cell apoptosis and oxidative stress induced by testicular ischemia-reperfusion. Iran J Basic Med Sci. 2017 Aug; 20(8): 905–911.
- Cakir M, Duzova H, Baysal I, Gül CC, Kuşcu G, Kutluk F, í‡akin H, Åžeker Åž, ć°lbeği E, Uslu S, Avci U, Demir S, Akinci C, Atli S. The effect of hypericum perforatum on kidney ischemia/reperfusion damage. Ren Fail. 2017 Nov;39(1):385-391
- Lai CC, Huang PH, Yang AH, Chiang SC, Tang CY, Tseng KW, Huang CH. Baicalein Attenuates Lung Injury Induced by Myocardial Ischemia and Reperfusion. Am J Chin Med. 2017;45(4):791-811.
- Sahu PK, Giri DD, Singh R, Pandey P, Gupta S, Shrivastava AK, et al. Therapeutic and medicinal uses of Aloe vera: A Review. Pharmacology & Pharmacy. 2013;4:599.
- Yang Y, Yang M, Ai F, Huang C. Cardioprotective Effect of Aloe vera Biomacromolecules Conjugated with Selenium Trace Element on Myocardial Ischemia-Reperfusion Injury in Rats. Biol Trace Elem Res. 2017 Jun;177(2):345-352.
- Yuksel Y, Guven M, Kaymaz B, Sehitoglu MH, Aras AB, Akman T, Tosun M, Cosar M. Effects of Aloe Vera on Spinal Cord Ischemia-Reperfusion Injury of Rats. J Invest Surg. 2016 Dec;29(6):389-398
- Schmelzer JD, Zochodne DW, Low PA. Ischemic and reperfusion injury of rat peripheral nerve. Proc Natl Acad Sci U S A. 1989 Mar; 86(5): 1639-42.
- Hao Z, Tiansheng S, Zhi L, Jianzheng Z, Xiaowei W, Jia L. Hip fracture aggravates systemic inflammation and lung injury in aged chronic cigarette smoke exposed rats. J Orthop Res. 2014;32:24-30. doi: 10.1002/jor.22491.
- Tüfek A, Tokgöz O, Aliosmanoglu I, Alabalik U, Evliyaoglu O, í‡iftçi T, et al. The protective effects of dexmedetomidine on the liver and remote organs against hepatic ischemia reperfusion injury in rats. Int J Surg. 2013;11:96-100. doi: 10.1016/j.ijsu.2012.12.003.
- Eppihimer MJ, Wolitzky B, Anderson DC, Labow MA, Granger DN. Heterogeneity of expression of E- and Pselectins in vivo. Circ Res 1996;79:560-569.
- Kiraz HA, Poyraz F, Kip G, Erdem í–, Alkan M, Arslan M, et al. The effect of levosimendan on myocardial ischemia-reperfusion injury in streptozotocin-induced diabetic rats. Libyan J Med. 2015 Dec 7;10:29269. doi: 10.3402/ljm.v10.29269.
- Granger DN, Korthuis RJ. Physiologic mechanisms of postischemic tissue injury. Annu Rev Physiol. 1995;57:311-332
- Davies SJ, Reichardt-Pascal SY, Vaughan D, Russel GI. Differential effect of ischemia- reperfusion injury on anti-oxidant enzyme activity in the rat kidney. Exp Nephrol. 1995;3:348-354.
- Choi SW, Son BW, Son YS, Park YI, Lee SK, Chung MH. The wound-healing effect of a glycoprotein fraction isolated from aloe vera. Br J Dermatol. 2001;145:535-545.
- Yagi A, Egusa T, Arase M, Tanabe M, Tsuji H. Isolation and characterization of the glycoprotein fraction with a proliferation-promoting activity on human and hamster cells in vitro from Aloe vera gel. Planta Med. 1997;63:18-21. http://dx.doi.org/10.1055/s-2006-957595
- Yagi A, Takeo S. [Anti-inflammatory constituents, aloesin and aloemannan in Aloe species and effects of tanshinon VI in Salvia miltiorrhiza on heart]. Yakugaku Zasshi. 2003;123:517- 532.
- Peng SY, Norman J, Curtin G, Corrier D, McDaniel HR, Busbee D. Decreased mortality of Norman murine sarcoma in mice treated with the immunomodulator, Acemannan. Mol Biother. 1991;3:79-87.
- West DP, Zhu YF. Evaluation of aloe vera gel gloves in the treatment of dry skin associated with occupational exposure. Am J Infect Control. 2003;31:40-42.
- Bazvand L, Aminozarbian MG, Farhad A, Noormohammadi H, Hasheminia SM, Mobasherizadeh S. Antibacterial effect of triantibiotic mixture, chlorhexidine gel, and two natural
materials Propolis and Aloe vera against Enterococcus faecalis: An ex vivo study. Dent Res J (Isfahan). 2014;11:469-474.
- Glatthaar-Saalmüller B, Fal AM, Schönknecht K, Conrad F, Sievers H, Saalmüller A. Antiviral activity of an aqueous extract derived from Aloe arborescens Mill. against a broad panel of viruses causing infections of the upper respiratory tract. Phytomedicine. 2015;22:911-920. doi: 10.1016/j.phymed.2015.06.006.
- Bernardes I, Felipe Rodrigues MP, Bacelli GK, Munin E, Alves LP, Costa MS. Aloe vera extract reduces both growth and germ tube formation by Candida albicans. Mycoses. 2012;55:257- 261. doi: 10.1111/j.1439-0507.2011.02079.x.
- Ayala A, Muñoz MF, Argüelles S. Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev. 2014;2014:360438. doi: 10.1155/2014/360438.
- Benson KF, Newman RA, Jensen GS. Antioxidant, anti-inflammatory, anti-apoptotic, and skin regenerative properties of an Aloe vera-based extract of Nerium oleander leaves (nae-8(®)). Clin Cosmet Investig Dermatol. 2015;8:239-248. doi: 10.2147/CCID.S79871.
- Saada HN, Ussama ZS, Mahdy AM. Effectiveness of Aloe vera on the antioxidant status of different tissues in irradiated rats. Pharmazie. 2003;58:929-931.
- Singh RP, Dhanalakshmi S, Rao AR. Chemomodulatory action of Aloe vera on the profiles of enzymes associated with carcinogen metabolism and antioxidant status regulation in mice. Phytomedicine. 2000;7:209-219.
- Guven M, Gölge UH, Aslan E, Sehitoglu MH, Aras AB, Akman T, Cosar M The effect of aloe vera on ischemia”Reperfusion injury of sciatic nerve in rats. Biomed Pharmacother. 2016 Apr;79:201-207.
- Knowles RG, Palacios M, Palmer RM, Moncada S. Formation of nitric oxide from Larginine in the central nervous system: a transduction mechanism for stimulation of the soluble guanylate cyclase. Proc Natl Acad Sci U S A. 1989;86:5159-5162.
- Marletta MA, Yoon PS, Iyengar R, Leaf CD, Wishnok JS. Macrophage oxidation of L- arginine to nitrite and nitrate: nitric oxide is an intermediate. Biochemistry. 1988;27:8706-8711.
- Papapetropoulos A, Rudic RD, Sessa WC. Molecular control of nitric oxide synthases in the cardiovascular system. Cardiovasc Res. 1999;43:509-520.
- Lamas S, Marsden PA, Li GK, Tempst P, Michel A. Endothelial nitric oxide synthase: molecular cloning and characterization of a distinct constitutive enzyme isoform. Proc Natl Acad Sci U S A. 1992;89:6348-6352.

References

- Maxwell SR, Lip GY. Reperfusion injury: a review of the pathophysiology, clinical manifestations and therapeutic options. Int J Cardiol. 1997;58:95-117.

- Carden DL, Granger DN. Pathophysiology of ischaemia-reperfusion injury. J Pathol.

;190:255-266.

- Collard CD, Gelman S. Pathophysiology, clinical manifestations, and prevention of ischemia-reperfusion injury. Anesthesiology. 2001;94:1133-1138.

- Nakamura T, Vollmar B, Winning J, Ueda M, Menger MD, Schäfers HJ. Heparin and the nonanticoagulant N-acetyl heparin attenuate capillary no-reflow after normothermic ischemia of the lung. Ann Thorac Surg. 2001;72:1183-1188.

- Nastos C, Kalimeris K, Papoutsidakis N, et al. Global consequences of liver ischemia/reperfusion injury. Oxid Med Cell Longev. 2014;2014, Article ID 906965, 13p.

- Ghasemnejad-berenji M, Ghazi-Khansari M, Yazdani I, Saravi SSS, Nobakht M, Abdollahi A, Ansari JM, Ghasemnejad-berenji H, Pashapour S, and Dehpour AR. Rapamycin protects testes against germ cell apoptosis and oxidative stress induced by testicular ischemia-reperfusion. Iran J Basic Med Sci. 2017 Aug; 20(8): 905–911.

- Cakir M, Duzova H, Baysal I, Gül CC, Kuşcu G, Kutluk F, í‡akin H, Åžeker Åž, ć°lbeği E, Uslu S, Avci U, Demir S, Akinci C, Atli S. The effect of hypericum perforatum on kidney ischemia/reperfusion damage. Ren Fail. 2017 Nov;39(1):385-391

- Lai CC, Huang PH, Yang AH, Chiang SC, Tang CY, Tseng KW, Huang CH. Baicalein Attenuates Lung Injury Induced by Myocardial Ischemia and Reperfusion. Am J Chin Med. 2017;45(4):791-811.

- Sahu PK, Giri DD, Singh R, Pandey P, Gupta S, Shrivastava AK, et al. Therapeutic and medicinal uses of Aloe vera: A Review. Pharmacology & Pharmacy. 2013;4:599.

- Yang Y, Yang M, Ai F, Huang C. Cardioprotective Effect of Aloe vera Biomacromolecules Conjugated with Selenium Trace Element on Myocardial Ischemia-Reperfusion Injury in Rats. Biol Trace Elem Res. 2017 Jun;177(2):345-352.

- Yuksel Y, Guven M, Kaymaz B, Sehitoglu MH, Aras AB, Akman T, Tosun M, Cosar M. Effects of Aloe Vera on Spinal Cord Ischemia-Reperfusion Injury of Rats. J Invest Surg. 2016 Dec;29(6):389-398

- Schmelzer JD, Zochodne DW, Low PA. Ischemic and reperfusion injury of rat peripheral nerve. Proc Natl Acad Sci U S A. 1989 Mar; 86(5): 1639-42.

- Hao Z, Tiansheng S, Zhi L, Jianzheng Z, Xiaowei W, Jia L. Hip fracture aggravates systemic inflammation and lung injury in aged chronic cigarette smoke exposed rats. J Orthop Res. 2014;32:24-30. doi: 10.1002/jor.22491.

- Tüfek A, Tokgöz O, Aliosmanoglu I, Alabalik U, Evliyaoglu O, í‡iftçi T, et al. The protective effects of dexmedetomidine on the liver and remote organs against hepatic ischemia reperfusion injury in rats. Int J Surg. 2013;11:96-100. doi: 10.1016/j.ijsu.2012.12.003.

- Eppihimer MJ, Wolitzky B, Anderson DC, Labow MA, Granger DN. Heterogeneity of expression of E- and Pselectins in vivo. Circ Res 1996;79:560-569.

- Kiraz HA, Poyraz F, Kip G, Erdem í–, Alkan M, Arslan M, et al. The effect of levosimendan on myocardial ischemia-reperfusion injury in streptozotocin-induced diabetic rats. Libyan J Med. 2015 Dec 7;10:29269. doi: 10.3402/ljm.v10.29269.

- Granger DN, Korthuis RJ. Physiologic mechanisms of postischemic tissue injury. Annu Rev Physiol. 1995;57:311-332

- Davies SJ, Reichardt-Pascal SY, Vaughan D, Russel GI. Differential effect of ischemia- reperfusion injury on anti-oxidant enzyme activity in the rat kidney. Exp Nephrol. 1995;3:348-354.

- Choi SW, Son BW, Son YS, Park YI, Lee SK, Chung MH. The wound-healing effect of a glycoprotein fraction isolated from aloe vera. Br J Dermatol. 2001;145:535-545.

- Yagi A, Egusa T, Arase M, Tanabe M, Tsuji H. Isolation and characterization of the glycoprotein fraction with a proliferation-promoting activity on human and hamster cells in vitro from Aloe vera gel. Planta Med. 1997;63:18-21. http://dx.doi.org/10.1055/s-2006-957595

- Yagi A, Takeo S. [Anti-inflammatory constituents, aloesin and aloemannan in Aloe species and effects of tanshinon VI in Salvia miltiorrhiza on heart]. Yakugaku Zasshi. 2003;123:517- 532.

- Peng SY, Norman J, Curtin G, Corrier D, McDaniel HR, Busbee D. Decreased mortality of Norman murine sarcoma in mice treated with the immunomodulator, Acemannan. Mol Biother. 1991;3:79-87.

- West DP, Zhu YF. Evaluation of aloe vera gel gloves in the treatment of dry skin associated with occupational exposure. Am J Infect Control. 2003;31:40-42.

- Bazvand L, Aminozarbian MG, Farhad A, Noormohammadi H, Hasheminia SM, Mobasherizadeh S. Antibacterial effect of triantibiotic mixture, chlorhexidine gel, and two natural

materials Propolis and Aloe vera against Enterococcus faecalis: An ex vivo study. Dent Res J (Isfahan). 2014;11:469-474.

- Glatthaar-Saalmüller B, Fal AM, Schönknecht K, Conrad F, Sievers H, Saalmüller A. Antiviral activity of an aqueous extract derived from Aloe arborescens Mill. against a broad panel of viruses causing infections of the upper respiratory tract. Phytomedicine. 2015;22:911-920. doi: 10.1016/j.phymed.2015.06.006.

- Bernardes I, Felipe Rodrigues MP, Bacelli GK, Munin E, Alves LP, Costa MS. Aloe vera extract reduces both growth and germ tube formation by Candida albicans. Mycoses. 2012;55:257- 261. doi: 10.1111/j.1439-0507.2011.02079.x.

- Ayala A, Muñoz MF, Argüelles S. Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev. 2014;2014:360438. doi: 10.1155/2014/360438.

- Benson KF, Newman RA, Jensen GS. Antioxidant, anti-inflammatory, anti-apoptotic, and skin regenerative properties of an Aloe vera-based extract of Nerium oleander leaves (nae-8(®)). Clin Cosmet Investig Dermatol. 2015;8:239-248. doi: 10.2147/CCID.S79871.

- Saada HN, Ussama ZS, Mahdy AM. Effectiveness of Aloe vera on the antioxidant status of different tissues in irradiated rats. Pharmazie. 2003;58:929-931.

- Singh RP, Dhanalakshmi S, Rao AR. Chemomodulatory action of Aloe vera on the profiles of enzymes associated with carcinogen metabolism and antioxidant status regulation in mice. Phytomedicine. 2000;7:209-219.

- Guven M, Gölge UH, Aslan E, Sehitoglu MH, Aras AB, Akman T, Cosar M The effect of aloe vera on ischemia”Reperfusion injury of sciatic nerve in rats. Biomed Pharmacother. 2016 Apr;79:201-207.

- Knowles RG, Palacios M, Palmer RM, Moncada S. Formation of nitric oxide from Larginine in the central nervous system: a transduction mechanism for stimulation of the soluble guanylate cyclase. Proc Natl Acad Sci U S A. 1989;86:5159-5162.

- Marletta MA, Yoon PS, Iyengar R, Leaf CD, Wishnok JS. Macrophage oxidation of L- arginine to nitrite and nitrate: nitric oxide is an intermediate. Biochemistry. 1988;27:8706-8711.

- Papapetropoulos A, Rudic RD, Sessa WC. Molecular control of nitric oxide synthases in the cardiovascular system. Cardiovasc Res. 1999;43:509-520.

- Lamas S, Marsden PA, Li GK, Tempst P, Michel A. Endothelial nitric oxide synthase: molecular cloning and characterization of a distinct constitutive enzyme isoform. Proc Natl Acad Sci U S A. 1992;89:6348-6352.

Author biographies is not available.