Nullification of aspirin induced gastrotoxicity and hepatotoxicity by prior administration of wheat germ oil in Mus musculus: histopathological, ultrastructural and molecular studies

H. R. H. Mohamed; S. R. Hamad

doi:10.14715/cmb/2017.63.8.25

Issue

Vol. 63 No. 8: Issue 8

Issue Published : September 4, 2017

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

Nullification of aspirin induced gastrotoxicity and hepatotoxicity by prior administration of wheat germ oil in Mus musculus: histopathological, ultrastructural and molecular studies

https://doi.org/10.14715/cmb/2017.63.8.25

H. R. H. Mohamed

Zoology Department, Faculty of Science, Cairo University, Giza, Egypt

S. R. Hamad

National Organization for Drug Control and Research (NODCAR), Ministry of Health and Population, Cairo, Egypt

Corresponding Author(s) : H. R. H. Mohamed

hananesresm@yahoo.com

Cellular and Molecular Biology, Vol. 63 No. 8: Issue 8
Article Published : August 30, 2017

Abstract

Aspirin (salicylic acetyl acid) is used worldwide to treat various inflammatory conditions and prevent cardiovascular disease, along with reducing the risk of cancer. However, administration of aspirin causes toxic effects, especially in the stomach and liver. Thus, our study examined the protective effect of wheat germ oil on aspirin-induced toxicity in the stomach and liver tissues of albino white mice. Administration of wheat germ oil before aspirin has restored normal hepatic and gastric tissue architecture and DNA integrity has become better than that of a negative health control group compared with the aspirin only treated group. The elevated gastric nitric oxide content in the aspirin only treated group was significantly decreased by wheat germ oil prior administration as a result of reducedthe expression of inducible nitric synthase and increased the expression of endothelial nitric oxide synthase compared to their expression in the aspirin administered group. Wheat germ oil pre-administration significantly reduced the level of malondialdehyde, increased the level of glutathione and decreased catalase and superoxide dismutase activities compared with those in aspirin only treated group. We conclude that wheat germ oil has a potential protective effect against aspirin induced gastro- and hepato-toxicity because of its free radical scavenging ability.

Keywords

Aspirin gastro-toxicity Hepatotoxicity Wheat germ oil Oxidative DNA damage Gastric nitric oxide.

Mohamed, H. R. H., & Hamad, S. R. (2017). Nullification of aspirin induced gastrotoxicity and hepatotoxicity by prior administration of wheat germ oil in Mus musculus: histopathological, ultrastructural and molecular studies. Cellular and Molecular Biology, 63(8), 120–130. https://doi.org/10.14715/cmb/2017.63.8.25

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References

Reference:
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Merchant, M. A. and Modi, D. N. (2004). Acute and chronic effects of aspirin on hematological parameters and hepatic ferritin expression in mice parameters and hepatic ferritin expression in mice. Indian J Pharmacol, Vol: 36, Issue 4 : 226-230.
Heibashy, M.I.; Mazen, G.M. and Ibrahim, M.A. (2014). Efficacy and Safety of some Medical Herbs on Gastric Ulcer Induced by Aspirin in Rats . Journal of Pharmacy and Biological Sciences, 9 (3): 19- 27.
Manzano, A. and Pérez-Segura, P. (2012). Colorectal cancer chemoprevention: is this the future of colorectal cancer prevention?. The Scientific World Journal. ID: 327341. 8 pages
Chan, A.T.; Arber, N.; Burn, J.; Chia, W.K.; Elwood, P.; Hull, M.A.; Logan, R.F.; Rothwell, P.M.; Schrör, K.; Baron, J.A. (2012). "Aspirin in the chemoprevention of colorectal neoplasia: an overview". Cancer Prevention Research (Philadelphia, PA). 5 (2): 164–78.
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Verdoodt, F.; Friis, S; Dehlendorff, C.; Albieri, V. and Kjaer, S.K. (2016). Non-steroidal anti-inflammatory drug use and risk of endometrial cancer: a systematic review and meta-analysis of observational studies. Gynecologic Oncology. 140 (2): 352–8.
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Saleh, Z. A. ; Ibrahim, K. S.; Farrag, A.-R. H. and Shaban, E. E. (2010). Effect of Carrot and Wheat Germ Oil Supplementation on antioxidant status of Rats Exposed to Benzene. polish journal of food and nutrition sciences (Pol. J. Food Nutr. Sci. Vol. 60, No. 2, pp. 175-181.
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Karabacak, M.; Kanbur, M.; Eraslan, G. and Sarıca, Z. S. (2011). The antioxidant effect of wheat germ oil on subchronic coumaphos exposure in mice. Ecotoxicology and Environmental Safety. , Vol.74 (7): 2119–2125
Ahmad, H.; Wadud, A.; Jahan, N. and Hasan, I. (2015). Anti-Ulcer Activity of Rhus Coriaria in Indomethacin and Water Immersion Restraint Induced Gastric Ulcer in Experimental Rats. International journal of medical sciences, Vol.7(3), pp. 61-66
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Tice, R.R., Agurell, E., Anderson, V., Burlinson, B., Hartmann, A., Kobayashi, H., Miyamae, Y., Rojas, E., Ryu, J.C., Sasaki, Y.F., (2000). Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen 35, 206e221.
Sriram, M.I., Kanth, S.B.M., Kalishwaralal, K., Gurunathan, S., (2010). Antitumor activity of silver nanoparticles in Dalton's lymphoma ascites tumor model. Int. J. Nanomed. 5, 753e762.
Laufs U, Gertz K, Dirnagl U, Bohm M, Nickenig G, Endres M. Rosuvastatin, (2002). A new HMG-CoA reductase inhibitor, upregulates endothelial nitric oxide synthase and protects from ischemic stroke in mice. Brain Res 2002; 942: 23–30.
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Beutler, E., Duron, O. and Kelly, M. B. (1963). Improved method for the determination of blood glutathione. J. Lab. Clin. Med. 61, 882–888.
Aebi, H. (1984). Catalase in vitro. Methods Enzymol. 105, 121–126.
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Adnan S, Janabi Al, Ahmad M, lzohyri A, Fouad K and Rubayai (2005). Pharmacological effects of low dose of aspirin on corpus luteum functions in mature cycling female mice, Institute of the Embryo Research and Infertility Treatment 10 (2), p150-162.
Fuster V and Sweeny JM (2011). Aspirin a historical and contemporary therapeutic overview. Circ; 123: 768-778.
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Bhattacharyya, S.; Ghosh, S. and Sil, P. C. (2014). Amelioration of Aspirin Induced Oxidative Impairment and Apoptotic Cell Death by a Novel Antioxidant Protein Molecule Isolated from the Herb Phyllanthus niruri. PLOS ONE | www.plosone.org. Volume 9 | Issue 2 | e89026.
Stadtman ER (2004). Role of oxidant species in aging. Curr Med Chem. 200411:1105–1112.
Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M (2006). Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact. 160:1–40.
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References

Reference:

Weisman, S.M. and Graham, D.Y. (2002). Evaluation of the benefits and risks of low-dose aspirin in the secondary prevention of cardiovascular and cerebrovascular events. Arch. Intern. Med. Oct 28; 162(19):2197-2202.

Merchant, M. A. and Modi, D. N. (2004). Acute and chronic effects of aspirin on hematological parameters and hepatic ferritin expression in mice parameters and hepatic ferritin expression in mice. Indian J Pharmacol, Vol: 36, Issue 4 : 226-230.

Heibashy, M.I.; Mazen, G.M. and Ibrahim, M.A. (2014). Efficacy and Safety of some Medical Herbs on Gastric Ulcer Induced by Aspirin in Rats . Journal of Pharmacy and Biological Sciences, 9 (3): 19- 27.

Manzano, A. and Pérez-Segura, P. (2012). Colorectal cancer chemoprevention: is this the future of colorectal cancer prevention?. The Scientific World Journal. ID: 327341. 8 pages

Chan, A.T.; Arber, N.; Burn, J.; Chia, W.K.; Elwood, P.; Hull, M.A.; Logan, R.F.; Rothwell, P.M.; Schrör, K.; Baron, J.A. (2012). "Aspirin in the chemoprevention of colorectal neoplasia: an overview". Cancer Prevention Research (Philadelphia, PA). 5 (2): 164–78.

Thun, M.J.; Jacobs, E.J. and Patrono, C. (2012). The role of aspirin in cancer prevention. Nature Reviews Clinical Oncology. 9 (5): 259–67.

Verdoodt, F.; Friis, S; Dehlendorff, C.; Albieri, V. and Kjaer, S.K. (2016). Non-steroidal anti-inflammatory drug use and risk of endometrial cancer: a systematic review and meta-analysis of observational studies. Gynecologic Oncology. 140 (2): 352–8.

Cuzick, J.; Thorat, M.A.; Bosetti, C; Brown, P.H.; Burn, J.; Cook, N.R.; Ford, L.G.; Jacobs, E.J.; Jankowski, J.A; La Vecchia, C.; Law, M.; Meyskens, F.; Rothwell, P.M; Senn, H.J. and Umar, A. (2014). Estimates of benefits and harms of prophylactic use of aspirin in the general population". Annals of Oncology. 26: 47–57.

Vad, N.M.; Kudugunti, S.K.; Wang, H.; Bhat, G.J. and Moridani, M.Y. (2014). Efficacy of acetylsalicylic acid (aspirin) in skin B16-F0 melanoma tumor-bearing C57BL/6 mice. Tumour Biol. 35(5):4967-76.

Matzke, G.R. (1996). Nonrenal toxicities of acetaminophen, aspirin and non-steroidal anti-inflammatory agents. Am J Kidney Dis; 28:63-70

McCredie, M.; Pommer, W.; McLaughlin, J.K.; Stewart, J.H.; Lindblad, P.; Mandel, J.S.; et al. (1995). International renal-cell cancer study. II. Analgesics. Int J Cancer; 60:345-349.

Wang, Z.; Hasegawa, J.; Wang, X.; Matsuda, A.; Tokuda, T. ; Miura, N. and Watanabe, T. (2011). Protective Effects of Ginger against Aspirin-Induced Gastric Ulcers in Rats. Yonago Acta medica; 54:11–19.

Sostres, C.; Gargallo,C.J. and Lanas,A.( 2013) . Nonsteroidal anti-infl ammatory drugs and upper and lower gastrointestinal mucosal damage . Arthritis Research & Therapy , 15(3):1-8 .

Mahmoud, A.A.; Mohdaly, A.A.A. and Elneairy, N.A.A. (2015). Wheat Germ: An Overview on Nutritional Value, Antioxidant Potential and Antibacterial Characteristics. Food and Nutrition Sciences, 6, 265-277.

Schmeda-Hirschmann, G. and Yesilada, E. (2005).Traditional medicine and gastroprotective crude drugs. J. Ethnopharmacol., 100(1-2):61-66.

Abid, S. A.; Mohammed, N. J. and Haider Abid Al-Ridha Al-Kaffaji. (2012). Effect of Liqurice and Chamomile Extracts in the Management of Gastric Ulcer in Rats. Medical Journal of Babylon-Vol. 9: 583-597.

Zhu, K.; Zhou, H. M. and Qian, H. F. (2006).Proteins extracted from defatted wheat germ, nutritional and structural properties. Cereal Chem.,; 83: 69–75).

Leenhardt, F.; Fardet, A.; Lyan, B.; Gueux, E.; Rock, E.; Mazur, A.; Chanliaud, E.; Demigne´ , C.and Re´me´ sy, C. (2008). Wheat germ supplementation of a low vitamin E diet in rats affords effective antioxidant protection in tissues. J. Am. Coll. Nutr.; 27, 222–228.

Hassanein, M.M. and Abedel-Razek, A.G. (2009). Chromatographic quantitation of some bioactive minor components in oils of wheat germ and grape seeds produced as by-products. J. Oleo Sci ; 58, 227–233.

Alessandri, J.M.; Extier, A.; AlGubory, K.H.; Harbeby, E.; Lallem, M.S.; Linard, A.; Lavialle, M. and Guesnet, P Influence of gender on DHA synthesis: The response of rat liver to low dietary a-linolenic acid evidences higher o3 D4- desaturation index in females, Eur. J. Nutr. 0208-1.

Niu, L.Y.; Jiang, S.T.and Pan, L.J. ( 2011). Preparation and evaluation of antioxidant activities of peptides obtained from defatted wheat germ by fermentation. J. Food Sci. Technol. doi:; 10.1007/s13197-011-0318-z.

Megahad, O. A. and El Kinawy, O. S. (2002). Studies on the extraction of wheat germ oil by commercial hexane". Grasas y Aceites. 53 (4): 414–418.

Mohamed DA, Ismael AI and Ibrahim AR (2005) Studying the anti-inflammatory and biochemical effects of wheat germ oil. Deutsche- Lebensmittel-Rundschau 101: 66.

Saleh, Z. A. ; Ibrahim, K. S.; Farrag, A.-R. H. and Shaban, E. E. (2010). Effect of Carrot and Wheat Germ Oil Supplementation on antioxidant status of Rats Exposed to Benzene. polish journal of food and nutrition sciences (Pol. J. Food Nutr. Sci. Vol. 60, No. 2, pp. 175-181.

Sliai. A M. (2015). Protective Effects ofWheat Germ Oil on Doxorubicin-Induced Hepatotoxicity in Male Mice. International Journal of Research Studies in Biosciences (IJRSB), Volume 3, Issue 6, PP 21-25. ISSN 2349-0357 (Print) & ISSN 2349-0365 (Online) www.arcjournals.org ©ARC Page | 21.

Bukhari1, M. H.; Khalil, J.; Qamar, S.; Qamar, Z.; Zahid, M.; Ansari1, N. and Bakhshi, I. M. (2011). Comparative Gastroprotective Effects of Natural Honey, Nigella sativa and Cimetidine against Acetylsalicylic Acid Induced Gastric Ulcer in Albino Rats. Journal of the College of Physicians and Surgeons Pakistan, Vol. 21 (3): 151-156.

Karabacak, M.; Kanbur, M.; Eraslan, G. and Sarıca, Z. S. (2011). The antioxidant effect of wheat germ oil on subchronic coumaphos exposure in mice. Ecotoxicology and Environmental Safety. , Vol.74 (7): 2119–2125

Ahmad, H.; Wadud, A.; Jahan, N. and Hasan, I. (2015). Anti-Ulcer Activity of Rhus Coriaria in Indomethacin and Water Immersion Restraint Induced Gastric Ulcer in Experimental Rats. International journal of medical sciences, Vol.7(3), pp. 61-66

Bancroft, J. and Gamble, M. (2002). "Theory and Practice of Histological Techniques." 5th (Ed.), Churchil Livingstone, London.

Tice, R.R., Agurell, E., Anderson, V., Burlinson, B., Hartmann, A., Kobayashi, H., Miyamae, Y., Rojas, E., Ryu, J.C., Sasaki, Y.F., (2000). Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen 35, 206e221.

Sriram, M.I., Kanth, S.B.M., Kalishwaralal, K., Gurunathan, S., (2010). Antitumor activity of silver nanoparticles in Dalton's lymphoma ascites tumor model. Int. J. Nanomed. 5, 753e762.

Laufs U, Gertz K, Dirnagl U, Bohm M, Nickenig G, Endres M. Rosuvastatin, (2002). A new HMG-CoA reductase inhibitor, upregulates endothelial nitric oxide synthase and protects from ischemic stroke in mice. Brain Res 2002; 942: 23–30.

Garhart CA, Heinzel FP, Czinn SJ, Nedrud JG (2003). Vaccine-induced r e d u c t i o n o f Helicobacter pylori colonization in mice is interleukin-12 dependent but gamma interferon and inducible nitric oxide synthase independent. Infect Immun; 71: 910–21.

Montogometry, H.A., Dymock, J.F., (1961). Analyst 86, 414.

Ohkawa, H., Ohishi, W., Yagi, K., (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95, 351e358.

Beutler, E., Duron, O. and Kelly, M. B. (1963). Improved method for the determination of blood glutathione. J. Lab. Clin. Med. 61, 882–888.

Aebi, H. (1984). Catalase in vitro. Methods Enzymol. 105, 121–126.

Nishikimi, M., Roa, N. A. and Yogi, K. (1972). Measurement of superoxide dismutase. Biochem. Biophys. Res. Commun. 46, 849–854.

Adnan S, Janabi Al, Ahmad M, lzohyri A, Fouad K and Rubayai (2005). Pharmacological effects of low dose of aspirin on corpus luteum functions in mature cycling female mice, Institute of the Embryo Research and Infertility Treatment 10 (2), p150-162.

Fuster V and Sweeny JM (2011). Aspirin a historical and contemporary therapeutic overview. Circ; 123: 768-778.

Seleem, H. S.; Ghobashy, H. A. and Zolfakar, A. S. (2010). Effect of Aspirin Versus Aspirin and Vitamin C on Gastric Mucosa (Fundus) of Adult Male Albino Rats. Histological and Morphometric Study. Egypt. J. Histol. Vol. 33, No. 2: 313 – 326

Olaibi, O. K. ; Ijomone, O. M. and Ajibade, A. J. (2014). Histomorphometric study of stomach and duodenum of aspirin treated Wistar rats. Journal of Experimental and Clinical Anatomy.Volume : 13 | Issue : 1 | Page : 12-16

Pohle, T.; Brzozowski, T.; Becker, J.C.; Van der Voort, I.R.; Markmann, A.; Konturek, S.J.; Moniczewski, A.; Domschke, W. and Konturek, J.W. (2001): Role of reactive oxygen metabolites in aspirin-induced gastric damage in humans: Gastroprotection by vitamin C. Aliment. Pharmacol. Ther. May; 15(5):677-687.

Kanter, M.; Demir, H.; Karakaya, C. and Ozbek, H. (2005): Gastroprotective activity of Nigella sativa L oil and its constituent, thymoquinone against acute alcohol-induced gastric mucosal injury in rats. World J. Gastroenterol. Nov. 14; 11(42):6662-6666.

Bhattacharyya, S.; Ghosh, S. and Sil, P. C. (2014). Amelioration of Aspirin Induced Oxidative Impairment and Apoptotic Cell Death by a Novel Antioxidant Protein Molecule Isolated from the Herb Phyllanthus niruri. PLOS ONE | www.plosone.org. Volume 9 | Issue 2 | e89026.

Stadtman ER (2004). Role of oxidant species in aging. Curr Med Chem. 200411:1105–1112.

Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M (2006). Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact. 160:1–40.

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Author biographies is not available.