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Vol. 69 No. 1: Issue 1

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Effects of miR-145 targeting rab5c and regulating MAPK / ERK signaling pathway on proliferation and invasion of thyroid papillary carcinoma cells

Effects of miR-145 on thyroid papillary carcinoma cells
https://doi.org/10.14715/cmb/2022.69.1.8
Xiaohui Wen
Department of Otorhinolaryngology Head and Neck Surgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, China
Zhanfeng Yan
Department of Otorhinolaryngology Head and Neck Surgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, China
Jingyan Du
Department of Otorhinolaryngology Head and Neck Surgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, China
Jinsheng Dai
Department of Otorhinolaryngology Head and Neck Surgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, China
Mingyu Liu
Department of Otorhinolaryngology Head and Neck Surgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, China
Xun Wang
Department of Otorhinolaryngology Head and Neck Surgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, China
Ningyu Wang
Department of Otorhinolaryngology Head and Neck Surgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, China

Corresponding Author(s) : Xun Wang

yuzhongque80923654@163.com

Cellular and Molecular Biology, Vol. 69 No. 1: Issue 1

Abstract

The objective of this research was to analyze the miR-145 function in thyroid papillary carcinoma cells and explore its possible mechanism. For this purpose, the TPC-1 cell line was selected, miR-145 overexpression and rab5c shRNA lentiviral vector were constructed, and transfected into PTC cells. Luciferase reporter gene was performed to determine the relationship between miR-145 and rab5c, Western blot and qPCR were performed to detach the expression of the related genes, CCK-8 cell proliferation assay and Transwell cell invasion assay were used to determine the proliferation and invasion ability of PTC-1 cells. Results showed that MiR-145 overexpression inhibited the wt-rab5c (wild-type rab5c)luciferase activity, decreased the expression of rab5c mRNA and protein levels in the TPC-1 cell line, inhibited the proliferation and invasion of PTC cell line TPC-1(P < 0.05). In TPC-1 cells, both miR-145 overexpression and RNA interference with rab5c could increase the expression of the p-ERK protein (P < 0.05). In conclusion, MiR-145 inhibits the proliferation and invasion of PTC cells by downregulating rab5c and activating MAPK/ERK pathway in vitro.

Keywords

miR-145 rab5c mapk/erk signaling pathway PTC
Wen, X., Yan, Z., Du, J., Dai, J., Liu, M., Wang, X., & Wang, N. (2023). Effects of miR-145 targeting rab5c and regulating MAPK / ERK signaling pathway on proliferation and invasion of thyroid papillary carcinoma cells: Effects of miR-145 on thyroid papillary carcinoma cells. Cellular and Molecular Biology, 69(1), 44–47. https://doi.org/10.14715/cmb/2022.69.1.8
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