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Copyright (c) 2023 Zhongtian Luo, Zhaobing Li, Manhua Li
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.The Effectiveness of Bax/Bcl-2 Gene Expression Ratio, Cardiac Function and Pulmonary Hemodynamics Indexes caused by Cardiopulmonary Rehabilitation Nursing
Corresponding Author(s) : Manhua Li
Cellular and Molecular Biology,
Vol. 68 No. 11: Issue 11
Abstract
This study aimed to observe the effects of cardiopulmonary rehabilitation nursing on gene expression, cardiac function and pulmonary hemodynamic indexes after percutaneous coronary intervention (PCI). A total of 104 coronary heart disease patients who received PCI treatment from January 2020 to January 2022 were selected by convenience sampling method. The patients were divided into control and observation groups by random number table method, with 52 cases in each group. The control group received routine nursing and the observation group received cardiopulmonary rehabilitation nursing. Cardiac function and pulmonary hemodynamics indexes were compared between the two groups. To evaluate gene expression, blood was taken from patients and healthy people after receiving complete information and obtaining consent. White blood cells were isolated by salting out. After RNA extraction and cDNA synthesis, the expression levels of Bcl2 and BAX genes were quantitatively measured by real-time PCR. Results showed that one month after discharge, the left ventricular end-diastolic diameter was decreased in the observation group compared with the control group and left ventricular ejection fraction and six minutes walk test grading were increased compared with the control group, showing a significant difference (P< 0.05). The pulmonary hemodynamic indexes were decreased in both groups compared with those at admission, but the pulmonary diastolic blood pressure, pulmonary systolic blood pressure, mean pulmonary artery pressure and pulmonary vascular resistance were lower in the observation group than in the control group of the same period, showing a difference (P< 0.05). The incidence of MACE was 1.92% (1/52) in the observation group, which was lower compared to the control group, showing a difference (P< 0.05). In this study, real-time PCR analysis showed that the expression ratio of Bcl2 to BAX genes in peripheral blood T cells in disease patients compared to healthy people was not different (P=0.07). In conclusion, Cardiopulmonary rehabilitation nursing for coronary heart disease after PCI can promote faster recovery of cardiac function, prolong exercise endurance and improve pulmonary hemodynamics indexes, demonstrating clinical reference value.
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