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Copyright (c) 2022 Ankita Bhosale, Rashmi Trivedi, Tarun Kumar Upadhyay, Dhruvi Gurjar, Faranak Aghaz, Fahad Khan, Pratibha Pandey, Md. Zeyaullah, Md. Shane Alam, Mohammad A A Al-Najjar, Samra Siddiqui
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.Investigation on Antimicrobial, Antioxidant, and Anti-cancerous activity of Agaricus bisporus derived β-Glucan particles against cervical cancer cell line
Corresponding Author(s) : Tarun Kumar Upadhyay
Cellular and Molecular Biology,
Vol. 68 No. 9: Issue 9
Abstract
Cervical cancer is one of the leading causes of death among women. Due to incomplete knowledge and hidden symptoms, it is not easily diagnosable. After the diagnosis of cervical cancer at an advanced stage, treatment such as chemotherapy and radiation therapy become too much costly along with having many side effects such as hair loss, loss of appetite, nausea, tiredness, etc. β-Glucan does a novel polysaccharide has many immunomodulatory properties. In our research, we have tested the efficacy of Agaricus bisporus derived β-Glucan particles (ADGPs) as an antimicrobial, antioxidant and anticancer agent against the cervical cancer HeLa cells. Prepared particles were quantified for carbohydrate content by anthrone test and further HPTLC analysis to confirm the polysaccharide nature and 1,3 glycosidic linkages of β-Glucan. ADGPs were found to have efficient antimicrobial activity against various fungal and bacterial tested strains. DPPH assay confirmed the antioxidant activity of ADGPs. Cell viability was assessed against the cervical cancer cell line by using the MTT and IC50 was found at 54μg/ml. Furthermore, β-Glucan was found to induce a significant amount of ROS, leading to the apoptosis of cells. The same was also assessed with the help of Propidium Iodide (PI) staining. With the help of JC-1 staining, β-Glucan was found to disrupt the Mitochondrial Membrane Potential (MMP), ultimately resulting in the cancer cell HeLa death. Based on our experimental findings, we found that ADGPs can be proven as an efficient therapy for cervical cancer treatment and work as an antimicrobial and antioxidant agent.
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