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Copyright (c) 2022 Wenming Zhang, Xiaodan Qian, Wei Chen
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.Evaluation of CD4/CD8 ratio in children with immune thrombocytopenic purpura (ITP) after treatment with intravenous immunoglobulin (IVIG)
Corresponding Author(s) : Wei Chen
Cellular and Molecular Biology,
Vol. 68 No. 5: Issue 5
Abstract
Intra Venous Immunoglobulin (IVIG) is a plasma-derived product used to treat many autoimmune diseases, including thrombocytopenia, immunodeficiency, and infectious diseases. In this study, the effect of IVIG injection was evaluated on the number of white blood cells, neutrophils, lymphocytes, and platelets. The effect of IVIG was also considered on the percentage of CD4 and CD8 positive cells T cell lymphocytes and their absolute number in pediatric patients with immune thrombocytopenic purpura. The study was a cross-sectional study performed on 32 patients with ITP. In these patients, a blood sample was taken before and one hour after the start of the IVG injection. For all samples, a complete blood cell, platelet count, and differential blood leukocyte count were performed by Sysmex kx-21. Then labeled anti-CD4 and anti-CD8 markers were used to evaluate the type of lymphocytes. SPSS software version 15 and a t-test with a significant level of p <0.05 were used for statistical analysis of the obtained results. Pearson correlation coefficient was also used to evaluate the relationship between patients' age and the total volume of injected IVIG results. Examination of blood cell counts showed a significant decrease in the mean of white blood cells, neutrophils, and lymphocytes after intravenous immunoglobulin injection. However, these changes were not statistically significant for platelets. A comparison of the mean percentage of CD4 and CD8 cells shows a significant increase in the CD4 / CD8 cell ratio after injection. The absolute number of CD4 and CD8 lymphocytes one hour after IVIG injection was significantly decreased, but their proportion increased after injection. Generally, IVIG reduces the absolute number of neutrophils, but this reduction is not associated with infection problems. This decrease is also seen in the number of lymphocytes. However, the change in the number and percentage of CD4 and CD8 cells depends on the sampling time following IVIG injection.
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