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Vol. 66 No. 3: Issue 3

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The synthesis of non-structural carbohydrates under the network security model

https://doi.org/10.14715/cmb/2020.66.3.2
Xiaohui Chai
School of Information Technology, Shangqiu Normal University, Shangqiu, 476000, China
Yuping Li
School of Information Technology, Shangqiu Normal University, Shangqiu, 476000, China

Corresponding Author(s) : Xiaohui Chai

jianchen1989@outlook.com

Cellular and Molecular Biology, Vol. 66 No. 3: Issue 3

Abstract

In order to explore the pathway of non-structural carbohydrate synthesis, an analysis of the pathway of non-structural carbohydrate synthesis under the network security model was proposed. Taking non-structural carbohydrates as the research object, the experimental materials and equipment were selected under the network security model. Through the establishment of detection methods, the preparation of freeze-dried carbohydrates, the influence of synthesis pathway-specific inhibitors on the synthesis of non-structural carbohydrates, the influence of precursors and intermediates in the pathway on the synthesis of non-structural carbohydrates, the effect of multiple factors on the synthesis of non-structural carbohydrates, the influence of polysaccharide synthesis, the treatment of reaction solution for detection, the preparation of detection sample, the detection conditions of a liquid phase, the detection conditions of LC-MS and the determination of carbohydrate biomass were studied. The results showed that the synthesis of nonstructural carbohydrates requires the participation of the glycolysis, shikimic acid and phenylpropane pathways, but not the polyketone pathway.

Keywords

Network Security Model Non-Structural Carbohydrate Synthetic Pathway
Chai, X., & Li, Y. (2020). The synthesis of non-structural carbohydrates under the network security model. Cellular and Molecular Biology, 66(3), 8–16. https://doi.org/10.14715/cmb/2020.66.3.2
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References
  1. Hwang H, Lee S, Lee P. Engineering and application of synthetic nar promoter for fine-tuning the expression of metabolic pathway genes in Escherichia coli. Biotech Biofuel 2018; 11(1), 103.
  2. DeMott CM, Majumder S, Burz DS, Reverdatto S, Shekhtman A. Ribosome mediated quinary interactions modulate in-cell protein activities. Biochem 2017; 56(32):4117-26.
  3. Wu R, Ma C, Zhang YH, Zhu Z. Complete oxidation of xylose for bioelectricity generation by reconstructing a bacterial xylose utilization pathway in vitro. Chem Cat Chem. 2018; 10(9):2030-5.
  4. Zhang HL, Zhang C, Pei CH, Han MN, Xu ZD, Li CH, Li W. Efficient production of trans"4"Hydroxy"l"proline from glucose by metabolic engineering of recombinant Escherichia coli. Let Appl Microbiol 2018; 66(5):400-8.
  5. Zhao Y, Wang Y, Gu Z, Wang Z. [3, 3]-Sigmatropic rearrangement of allenic alcohols: stereoselective synthesis of 1, 3-diene-2-ol sulfonates. Organic Biomol Chem 2017; (18):4014-21.
  6. Cui D, Zhang Ch, Wu K, Xie Zh. Formal total synthesis of salvianolic acid N. Organic Biomol Chem 2018; 16(5): 832-7.
  7. Papadimitriou M, Mountzios G, Papadimitriou CA. The role of PARP inhibition in triple-negative breast cancer: unraveling the wide spectrum of synthetic lethality. Cancer Treat Rev 2018; 1; 67:34-44.
  8. Broecker F, Götze S, Hudon J, Rathwell DC, Pereira CL, Stallforth P, Anish C, Seeberger PH. Synthesis, liposomal formulation, and immunological evaluation of a minimalistic carbohydrate-α-GalCer vaccine candidate. J Med Chem 2018; 61(11):4918-27.
  9. Adokoh CK, Darkwa J, Kinfe HH. Synthesis, characterization and anticancer evaluation of phosphinogold (I) thiocarbohydrate complexes. Polyhedron. 2017; 138:57-67.
  10. Keshipour S, Mirmasoudi SS. Cross-linked chitosan aerogel modified with au: synthesis, characterization and catalytic application. Carbohydrate Polymers, 2018; 196: 494-500.
  11. Fan Z, Qin Y, Liu S, Xing R, Yu H, Chen X, Li K, Li P. Synthesis, characterization, and antifungal evaluation of diethoxyphosphoryl polyaminoethyl chitosan derivatives. Carbohydrate polymers. 2018; 190:1-1.
  12. Goncalves FJ, Kamal F, Gaucher A, Gil R, Bourdreux F, Martineau-Corcos C, Gurgel LV, Gil LF, Prim D. Synthesis, characterisation and application of pyridine-modified chitosan derivatives for the first non-racemic Cu-catalysed Henry reaction. Carbohydrate Polymer 2018; 181:1206-12.
  13. Wu Z, Zhao D, Li S, Wang J, Bi C, Zhang X. Combinatorial modulation of initial codons for improved zeaxanthin synthetic pathway efficiency in Escherichia coli. Microbiol Open. 2019; 8(12): e930.
  14. Wu T, Zhang B, Bi C. Improving β-carotene production in Escherichia coli by modularized regulation of the membrane synthetic pathway and morphology engineering. Chin J Biotechnol 2018; 34(5), 703-711.
  15. He R, Zhou H. Time-domain substructure transient vibration transfer path analysis based on time-varying frequency response functions under operational excitations. Shock and Vibration 2019(3): 1-16.
  16. Li H, Bloom JM, Farjoun Y, Fleharty M, Gauthier L, Neale B, MacArthur D. A synthetic-diploid benchmark for accurate variant-calling evaluation. Nat Methods. 2018; 15(8):595-7.
  17. Mercy G, Mozziconacci J, Scolari VF, Yang K, Zhao G, Thierry A, Luo Y, Mitchell LA, Shen M, Shen Y, Walker R. 3D organization of synthetic and scrambled chromosomes. Sci 2017; 355(6329): eaaf4597.
  18. Chen G. Engineering the ribosomal DNA in a megabase synthetic chromosome. Sci 2017; 355(6329).
  19. Saadati Jebeli M, Kahrizi D, Nosratti I. The response of transgenic Brassica napus with aroA gene to glyphosate treatment. Genetic Eng Biosafety J 2018;7(1):41-52.
  20. Taravat E, Zebarjadi A, Kahrizi D, Yari K. Isolation, cloning, and characterization of a partial novel aro A gene in common reed (Phragmites australis). Pharmaceutic Biol 2015;53(5):637-41.
  21. Kahrizi D. Reduction of EPSP synthase in transgenic wild turnip (Brassica rapa) weed via suppression of aroA. Mol Biol Rep 2014; 41: 8177-8184.
  22. Motamedi J, Zebarjadi A, Kahrizi D, Salmanian AH. Transformation of bacterial mutated aroA gene to safflower (Carthamus tinctorius L.) via Agrobacterium-mediated method. Modern Genetics J 2012; 7(1): 57-63.
  23. Motamedi J, Zebarjadi A, Kahrizi D, Salmanian AH. In vitro propagation and Agrobacterium-mediated transformation of safflower (Carthamus tinctorius L.) using a bacterial mutated aroA gene. Aust J Crop Sci 2011; 4(5): 479-486.
  24. Bordbar M, Darvishzadeh R, Pazhouhandeh M, Kahrizi D. An overview of genome editing methods based on endonucleases. Modern Genetics J 2020; 15(2): 75-92.
  25. Kahrizi D, Salmanian AH. Substitution of Ala183Thr in aroA Product of E. coli (k12) and Transformation of Rapeseed (Brassica napus) with Altered Gene to Tolerance of Plant to Roundup. Transgenic Plant J 2008; 2(2): 170-175.
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