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Vol. 65 No. 5: Issue 5

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Emodin attenuates Alzheimer's disease by activating the protein kinase C signaling pathway

https://doi.org/10.14715/cmb/2019.65.5.5
Changwang Du
Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiaotong University. No. 277 Yanta West Road, Xi'an, Shaanxi Province, 710061, China
Luoning Shi
Department of Kidney Transplantation, The First Affiliated Hospital of Xi'an Jiaotong University. No. 277 Yanta West Road, Xi'an, Shaanxi Province, 710061, China
Mei Wang
Department of Health Information Services, The First Affiliated Hospital of Xi'an Jiaotong University. No. 277 Yanta West Road, Xi'an, Shaanxi Province, 710061, China
Ping Mao
Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiaotong University. No. 277 Yanta West Road, Xi'an, Shaanxi Province, 710061, China
Jia Wang
Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiaotong University. No. 277 Yanta West Road, Xi'an, Shaanxi Province, 710061, China
Yanqiang Wei
Department of Surgery, The First Affiliated Hospital of Xi'an Jiaotong University. No. 277 Yanta West Road, Xi'an, Shaanxi Province, 710061, China
Juanru Hou
Department of Surgery, The First Affiliated Hospital of Xi'an Jiaotong University. No. 277 Yanta West Road, Xi'an, Shaanxi Province, 710061, China
Maode Wang
Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiaotong University. No. 277 Yanta West Road, Xi'an, Shaanxi Province, 710061, China

Corresponding Author(s) : Maode Wang

jjht98@163.com

Cellular and Molecular Biology, Vol. 65 No. 5: Issue 5

Abstract

To investigate the effects of emodin on learning and memory and protein kinase C (PKC) signaling pathway in Alzheimer's disease (AD) model mice. 60 APP/PS1 double transgenic AD mice were selected as model mice at the age of 7-8 months, 36 healthy male C57BL/6 mice served as the control group. Morris water maze method and passive avoidance experiment were used to evaluate the memory ability of mice. The thiazole blue (MTT) method and the lactate dehydrogenase (LDH) cytotoxicity test kit were used to evaluate the effect of emodin on the cell viability of hippocampal neurons in HT22 mice treated with β-amyloid peptide 1-42 (Aβ1-42). The effect of emodin on PKC levels was explored using the modified Takai method and Western blotting. Behavioral test results showed that the escape latency of the mice in the model group was longer than that in the control group (P<0.05), and the escape latency was significantly shortened given a emodin prognosis. The MTT and LDH test results showed that emodin to Aβ- overexpression induced the protective effect of hippocampus cells in HT22 mice. Western blot analysis showed that the phosphorylation level of PKC in mice increased significantly after emodin administration. Emodin can attenuate oxidative stress and inflammatory response in Alzheimer's model mice by activating PKC pathway, thereby improving cognitive function.

Keywords

Emodin Alzheimer's disease Protein kinase C Learning and memory ability HT22 Western blot.
Du, C., Shi, L., Wang, M., Mao, P., Wang, J., Wei, Y., Hou, J., & Wang, M. (2019). Emodin attenuates Alzheimer’s disease by activating the protein kinase C signaling pathway. Cellular and Molecular Biology, 65(5), 32–37. https://doi.org/10.14715/cmb/2019.65.5.5
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