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Vol. 65 No. 4: Issue 4

Palmatine plays a role in sedation and hypnosis by increasing 5-hydroxytryptamine

https://doi.org/10.14715/cmb/2019.65.4.11
Jianwei Du
Department of Anesthesiology, The First Affiliated Hospital of Nanchang University, Nanchang City, China
Yanbo Yu
Department of Pain, The First Affiliated Hospital of Nanchang University, Nanchang City, China
Liuji Qiu
Department of Anesthesiology, Huizhou Central People's Hospital, Huizhou City, China
Mizhen Qiu
Department of Pain, Ganzhou People's Hospital, Ganzhou City, China
Qing Zhu
Department of Pain, Ganzhou People's Hospital, Ganzhou City, China

Corresponding Author(s) : Liuji Qiu

nicwd0@163.com

Cellular and Molecular Biology, Vol. 65 No. 4: Issue 4

Abstract

Objective of this study was to investigate the sedative and hypnotic effects of palmatine and to observe whether its mechanism is related to 5-hydroxytryptamine (5-HT) and GABA. The sedative and hypnotic effects of palmatine on mice were observed with mouse autonomic activity test, direct sleep test, pentobarbital sodium in suprathreshold and subthreshold dose sleep test. The content of GABA and 5-HT in brain homogenate was determined by ELISA  method. Mouse brain specimens were observed by immunohistochemistry for 5-HT expression in the nucleus of mouse brain. Palmatine could reduce spontaneous activities of mice, prolong the sleep time of mice induced by pentobarbital sodium in suprathreshold dose and shorten the sleep latency.  And it could increase the number of mice falling asleep induced by pentobarbital sodium in subthreshold dose and the incidence of falling asleep, but with no direct sleep effect. In addition, it enhanced the 5-HT content in brain, but had no effect on GABA content, and had no toxicity to PC12 cells. Palmatine plays a significant role in sedation and hypnosis, which may be associated with the increase of intra-cerebral 5-HT.

Keywords

Palmatine Sedation Sleeping improvement 5-HT GABA.
Du, J., Yu, Y., Qiu, L., Qiu, M., & Zhu, Q. (2019). Palmatine plays a role in sedation and hypnosis by increasing 5-hydroxytryptamine. Cellular and Molecular Biology, 65(4), 69–75. https://doi.org/10.14715/cmb/2019.65.4.11
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