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Vol. 65 No. 3: Issue 3

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Effects of methyl jasmonate and phloroglucinol on thebaine and sanguinarine production in cell suspension culture of Persian poppy (Papaver bracteatum Lindl.)

https://doi.org/10.14715/cmb/2019.65.3.2
Taraneh Dastmalchi
Department of Plant Breeding and Biotechnology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Mansour Omidi
College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
Reza Azizinezhad
Department of Plant Breeding and Biotechnology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Shamsali Rezazadeh
Department of Pharmacognosy, Institute of Medicinal Plants, ACECR, Karaj, Iran
Alireza Etminan
Department of Plant Breeding, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran

Corresponding Author(s) : Mansour Omidi

momidi@ut.ac.ir

Cellular and Molecular Biology, Vol. 65 No. 3: Issue 3

Abstract

The biosynthesis path engineering could be very promising for mass production of alkaloids by applying elicitors in the cell suspension culture of Persian poppy (Papaver bracteatum Lindl.). In this work, the effects of different concentrations of methyl jasmonate (MJ) and phloroglucinol (PG) on thebaine and sanguinarine productions in vitro were investigated. Roots as explant and supplementing 3 mg L–1 2,4-Dichlorophenoxyacetic acid with 0.5 mg L–1 Benzyl amino purine to modified MS medium were selected to achieve the most efficient combination for callus induction and production of callus fresh and dry weights. At 48 h after treatment, the addition of PG and MJ individually and in combination together significantly increased both thebaine and sanguinarine contents than the control. The results of high-performance liquid chromatography (HPLC) detection indicated that the highest production rate has been achieved through a synergic effect of two elicitors after 48 h. Results revealed that adding 200 μM of MJ and 100 mg L–1 PG increased thebaine and sanguinarine contents by 56.36 and 107.71–fold than control cells, respectively.

Keywords

Cell suspension culture Methyljasmonate Phloroglucinol Sanguinarine Thebaine Papaver bracteatum Lindl.
Dastmalchi, T., Omidi, M., Azizinezhad, R., Rezazadeh, S., & Etminan, A. (2019). Effects of methyl jasmonate and phloroglucinol on thebaine and sanguinarine production in cell suspension culture of Persian poppy (Papaver bracteatum Lindl.). Cellular and Molecular Biology, 65(3), 11–17. https://doi.org/10.14715/cmb/2019.65.3.2
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