Bio-insecticidal effects of Oleaster leaves aqueous extracts against Psylla larvae (Euphyllura olivina (Costa)), a primary pest of Olea europaea L.

Nadjet G. Mestar; Malika N. Boudiaf; Souad Lahcene; Hocine Abbaci; Ghenima I. Aiche; Boussad Metna; Noria S. Saadoun; Faiza Taibi; Karim Houali

doi:10.14715/cmb/2017.64.15.6

Issue

Vol. 64 No. 15: Issue 15

Issue Published : January 1, 2019

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Bio-insecticidal effects of Oleaster leaves aqueous extracts against Psylla larvae (Euphyllura olivina (Costa)), a primary pest of Olea europaea L.

https://doi.org/10.14715/cmb/2017.64.15.6

Nadjet G. Mestar

Laboratory of analytic biochemistry research and biotechnology (LABAB). Department of Biochemistry and Microbiology, Faculty of Biological Sciences and Agronomic Sciences. Mouloud Mammeri University, Tizi-Ouzou, 15000, Algeria

Malika N. Boudiaf

Laboratoire Resources Naturelles (LRN). Department of Agronomy, Faculty of Biological Sciences and Agronomic Sciences. Mouloud Mammeri University, Tizi-Ouzou, 15000, Algeria

Souad Lahcene

Hocine Abbaci

Laboratory of Mastery of Renewable Energies, University of Bejaia, Targa Ouzemmour, 06000 Bejaia. Algeria

Ghenima I. Aiche

Boussad Metna

Laboratoire Resources Naturelles (LRN). Department of Agronomy, Faculty of Biological Sciences and Agronomic Sciences. Mouloud Mammeri University, Tizi-Ouzou, 15000, Algeria

Noria S. Saadoun

Laboratoire Resources Naturelles (LRN). Department of Agronomy, Faculty of Biological Sciences and Agronomic Sciences. Mouloud Mammeri University, Tizi-Ouzou, 15000, Algeria

Faiza Taibi

Research Laboratory on the Biodiversity and Ecosystem pollution. Department of Agronomy, Nature and Life Sciences Faculty, University Chadli Bendjedid El-Tarf, 36000, Algeria

Karim Houali

Corresponding Author(s) : Nadjet G. Mestar

n.mestar@yahoo.com

Cellular and Molecular Biology, Vol. 64 No. 15: Issue 15
Article Published : December 31, 2018

Abstract

Many plant species produce phenolic compounds in their various organs and their use in crop protection. These plant secondary metabolites may serve as toxins against the insect pests. The objective of this study was to evaluate in vitro the bio-insecticidal effect of an aqueous extract of wild Olive leaves on Psylla larvae (Euphyllura olivina), a primary pest of the cultivated Olive tree (Olea europaea L. subsp sativa). Two concentrations of 0.05g/ml and 0.1g/ml leaves grinding powder in distilled water were sprayed on branches infested with Psylla larvae. The obtained results revealed a very significant mortality rate of the larvae 24 hours after spraying. The chemical composition of Oleaster leaves aqueous extracts is determined by HPLC-DAD. The results show in majority the presence of phenolic compounds represented by oleuropein and its metabolite hydroxytyrosol. The phenolic compounds of the crude extract were at the origin of this mortality. The Analysis of Variance revealed highly significant results both between the sampled trees and between the tested concentrations. The Principal Component Analysis (PCA) revealed a close relation between the physiological state of the studied trees and the degree of their infestation by the phytophagus. Taking into account, the physical and chemical characteristics of the sampled soils, data analysis showed that trees growing on nitrogen-rich soils were more infested than those growing on soils rich in organic carbon (Corg) and phosphorus (Porg).

Keywords

Olea europaea subsp europaea var sylvestris Bio insecticide Euphyllura olivina Aqueous extract Polyphenols Soil.

Mestar, N. G., Boudiaf, M. N., Lahcene, S., Abbaci, H., Aiche, G. I., Metna, B., Saadoun, N. S., Taibi, F., & Houali, K. (2018). Bio-insecticidal effects of Oleaster leaves aqueous extracts against Psylla larvae (Euphyllura olivina (Costa)), a primary pest of Olea europaea L. Cellular and Molecular Biology, 64(15), 35–40. https://doi.org/10.14715/cmb/2017.64.15.6

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References

Kant MR, Jonckheere W, Knegt B, Lemos F, Liu J, Schimmel BCJ, Villarroel CA, Ataide LMS, Dermauw W, Glas JJ, Egas M, Janssen A, Van Leeuwen T, Schuurink RC, Sabelis MW, Alba JM. Mechanisms and ecological consequences of plant defence induction and suppression in herbivores communities. Ann. Bot. 2015; 115: 1015-1051.
Meftah H, Boughdad A, Bouchelta A. Effet biocide des extraits aqueux bruts de Capsicumfutescens, Melia azedarach et Peganum harmala sur Euphylluraolivina Costa (Homoptera, Psyllidae) en verger. Cahier d'Agriculture. 2011; 20(6): 463-467.
Quézel P, Médail F. Ecologie et biogéographie des foríªts du bassin méditerranéen. Editions scientifiques et médicales Elsevier SAS 2003: 112-114.
Breton C, Medail F, Pinatel C, Bervillé A. De l'olivier í l'oléastre : origine et domestication de l'Olea europaeaL. dans le bassin méditerranéen. Cahier d'Agriculture. 2006 ; 15(4), 329-336.
Del Bene G, Gargani E, Landi S. Observations on the life cycle and diapause of Euphylluraolivina (Costa) and Euphyllura phillyreae. Foerster (HomopteraAphalaridae). Adv Horti Sci. 1997; 10-16.
Chermiti B, Onillon JC. Essais d'evalution des dégí¢ts causés par la génération automnale du psylle de l'olivier: Euphylluraolivina (Costa) (Homoptera, Aphalaridae) Hedadra (Monastir,Tunisia). Mededeling en Faculte it Landbouw kundigeen Toegepaste Biologische Wetenschappen. Universite it Gent. 1993
Mustafa TM. Factors affecting the distribution of Euphylluraolivina Costa (Hom.,Psyllidae) on olive. J Appl Entomol. 1984; 97(1-5): 371-375.
Chermiti B. Approche d'évaluation de la nocivité du psylle de l'olivier Euphyllura Olive (Costa) (Homoptera, Aphalaridae). Olivae. 1992 ; 43, 34-42.
Ouguas Y, Hilal A, El Hadrami I. Effet biocide des extraits phénoliques oléicoles sur les adultes du psylle de l'olivier Euphylluraolivina Costa (Homoptera : Psyllidae) sur deux variétés d'oliviers Menara et Aberquine au Maroc. Revue Ezzaitouna. 2010; 11(1): 1-15.
Zouiten N, El Hadrami I. Le psylle de l'olivier: état des connaissances et perspectives de lutte. Cahier d'Agriculture. 2001;10(4) : 225-32.
Boudiaf Nait Kaci M, Hedde M, Mouas Bourbia S, Derridj A. Hierarchization of factors driving soil macrofauna in north Algeria groves. Biotech. Agron. Soc. Env. BASE. 2014; 18 (1), 11-18.
Jackson ML. 1967. Soil chemical analysis. Prentice Hall. Inc., Englewood Cliff. N.J., Library of Congress, USA.
WRB World Reference Base for Soil Resources. 1998.AISS, IRSIC, FAO. Rome ,90p.
Hinsinger P, Plassard C, Tang C, Jaillard B. Origins of root-mediated pH changes in the rhisosphere and their response to environmental constraints: A Rev. Plant and soil. 2003; 248: 43-59.
Gargouri K, Mhiri A. Relationship between soil fertility. Phosphorus and Potassium nutrition of the olive groves in Tunisia. Ed. I.N.A., Tunisia. 2002
Mouas Bourbia S, Barré P, Boudiaf Nait Kaci M, Derridj A, Velde V. Potassium status in bulk and rhizospheric soils of olive groves in north Algeria. Geoderma. 2013; 161-168.
Larif M, Zarrouk A, Soulaymani A, Elmidaoui A. New innovation in order to recover the polyphenols of olive mill wastewater extracts for use as a bio pesticide against the Euphylluraolivina and Aphis citricola. Res Chem Intermed. 2013; 39(9): 4303-4313.
Dibou A, Ksantini M, Raptopoulos D. Essais de l'efficacité du bio insecticide tétrastop en vue d'un contrí´le biologique du psylle de l'olivier Euphyllura olivina Costa (Homoptera, Psyllidae). Rev. Ezz. 2010; 11(1): 1-10.
Feeny P. Seasonal changes in Oak leaf Tannins and nutrients as a cause of spring feeding by winter moth caterpillars. Ecology. 1970; 51(4), 565-581.
Bryant JP, Chapin FS, Klein DR. Carbon/nutrient balance of Boreal plants in relation to vertebrate herbivory. OIKOS. 1983; 40, 357-368.
Ben Hamouda A, Boussadia O, Khaoula B, Laarif A, Braham M. Studies on insecticidal and deterrent effects of olive leaf extracts on Myzus persicae and Phthorimaea operculella. J EZS. 2015; 3(6): 294-297.
Ortega-García F, Peragón J. HPLC analysis of oleuropein, hydroxytyrosol, and tyrosol in stems and roots of Olea europaea L. cv. Picual during ripening. J Sci Food Agric. 2011; 90: 2295-2300.
Fernández-Bolaños JG, López í“, López-García Mí, Marset A. 2012. Biological Properties of Hydroxytyrosol and Its Derivatives. Olive Oil Constituents, Quality, Health Properties and Bioconversions, Dimitrios Boskou (Ed.), InTech. ISBN: 978-953-307-921-9: 375-396.
Al-Rimawi F. Development and validation of a simple reversedphase HPLC-UV method for determination of oleuropein in olive leaves. J Food Drug Anal. 2014; 22: 285-289.
Katsoulieris EN. The olive leaf extract oleuropein exerts protective effects against oxidant-induced cell death, concurrently displaying pro-oxidant activity in human hepatocarcinoma cells. Redox Rep. 2016; 21(2): 90-97.
Čepo DV, Albahari P, Konćić MZ, Radić K, Jurmanović S, Jug M. Solvent extraction and chromatographic determination of polyphenols in olive pomace. Food in Health and Disease. J Nutr Diet. 2017; (1) 7-14
Belaqziz M. Tan SP, El-Abbassi A, Kiai H, Hafidi A, O'Donovan O, McLoughlin P. Assessment of the antioxidant and antibacterial activities of different olive processing wastewaters. PLoS One. 2017; 12(9): 1-16.
Malik NSA, Joe M, Bradford JM. Changes in oleuropein levels during differentiation and development of floral buds in ‘Arbequina' olives. Sci Hortic. 2006; 110: 274–278
Koudounas K, Banilas G, Michaelidis Ch, Demoliou C, Rigas S, Hatzopoulos P. A defence-related Olea europaea Î²-glucosidase hydrolyses and activates oleuropein into a potent protein cross-linking agent. J Exp Bot. 2015; 66(7): 2093–2106.
Konno K, Hirayama C, Yasui H, Nakamura M. Enzymatic activation of oleuropein: a protein crosslinker used as a chemical defence in the privet tree. Pro Natl Acad Sci. 1999; 96: 9159–9164.
Hassan A. Effects of mineral nutrients on physiological and biochemical processes related to secondary metabolites production in medicinal herbs. Medicinal and Aromatic Plant Sci and Biotech. 2012; 6(1), 105-110.
Duarte AR, Santos SC, Seraphin JC, Ferri PH. Influence of spatial, edaphic and genetic factors on phenols and essential oils of Myrciaria cauliflora fruits. J. Braz. Chem. Soc. 2012; 23(4): 737-746.
Orians CM, Lower S, Fritz RS, Roche BM. The effects of plant genetic variation and soil nutrients on secondary chemistry and growth in shrubby willow, Salix sericea : patterns and constraints on the evolution of resistance traits. Biochem Syst Ecol. 2003; 31: 233-247.
Di Ferdinando M, Brunetti C, Agati G, Tattini M. Multiple functions of polyphénols in plants inhabiting unfavorable Mediterranean areas. Env Exp Bota. 2014; 103: 107-116.
Radix P, Bastien C, Jay C, Charlot G, Seigle F. The influence of soil nature on polyphenols in walnut tissues. A possible explanation of differences in the expression of walnut blight. Inra/Elsevier, Paris. Agronomie. 1988; 18: 627- 637.
Estiarte M, Filella I, Serra J, Peñuelas J. Effects of nutrients and water stress on leaf phenolic content of peppers and susceptibility to generalist herbivore Helicoverpa armigera (Hubner). Springer-Verlag

References

Kant MR, Jonckheere W, Knegt B, Lemos F, Liu J, Schimmel BCJ, Villarroel CA, Ataide LMS, Dermauw W, Glas JJ, Egas M, Janssen A, Van Leeuwen T, Schuurink RC, Sabelis MW, Alba JM. Mechanisms and ecological consequences of plant defence induction and suppression in herbivores communities. Ann. Bot. 2015; 115: 1015-1051.

Meftah H, Boughdad A, Bouchelta A. Effet biocide des extraits aqueux bruts de Capsicumfutescens, Melia azedarach et Peganum harmala sur Euphylluraolivina Costa (Homoptera, Psyllidae) en verger. Cahier d'Agriculture. 2011; 20(6): 463-467.

Quézel P, Médail F. Ecologie et biogéographie des foríªts du bassin méditerranéen. Editions scientifiques et médicales Elsevier SAS 2003: 112-114.

Breton C, Medail F, Pinatel C, Bervillé A. De l'olivier í l'oléastre : origine et domestication de l'Olea europaeaL. dans le bassin méditerranéen. Cahier d'Agriculture. 2006 ; 15(4), 329-336.

Del Bene G, Gargani E, Landi S. Observations on the life cycle and diapause of Euphylluraolivina (Costa) and Euphyllura phillyreae. Foerster (HomopteraAphalaridae). Adv Horti Sci. 1997; 10-16.

Chermiti B, Onillon JC. Essais d'evalution des dégí¢ts causés par la génération automnale du psylle de l'olivier: Euphylluraolivina (Costa) (Homoptera, Aphalaridae) Hedadra (Monastir,Tunisia). Mededeling en Faculte it Landbouw kundigeen Toegepaste Biologische Wetenschappen. Universite it Gent. 1993

Mustafa TM. Factors affecting the distribution of Euphylluraolivina Costa (Hom.,Psyllidae) on olive. J Appl Entomol. 1984; 97(1-5): 371-375.

Chermiti B. Approche d'évaluation de la nocivité du psylle de l'olivier Euphyllura Olive (Costa) (Homoptera, Aphalaridae). Olivae. 1992 ; 43, 34-42.

Ouguas Y, Hilal A, El Hadrami I. Effet biocide des extraits phénoliques oléicoles sur les adultes du psylle de l'olivier Euphylluraolivina Costa (Homoptera : Psyllidae) sur deux variétés d'oliviers Menara et Aberquine au Maroc. Revue Ezzaitouna. 2010; 11(1): 1-15.

Zouiten N, El Hadrami I. Le psylle de l'olivier: état des connaissances et perspectives de lutte. Cahier d'Agriculture. 2001;10(4) : 225-32.

Boudiaf Nait Kaci M, Hedde M, Mouas Bourbia S, Derridj A. Hierarchization of factors driving soil macrofauna in north Algeria groves. Biotech. Agron. Soc. Env. BASE. 2014; 18 (1), 11-18.

Jackson ML. 1967. Soil chemical analysis. Prentice Hall. Inc., Englewood Cliff. N.J., Library of Congress, USA.

WRB World Reference Base for Soil Resources. 1998.AISS, IRSIC, FAO. Rome ,90p.

Hinsinger P, Plassard C, Tang C, Jaillard B. Origins of root-mediated pH changes in the rhisosphere and their response to environmental constraints: A Rev. Plant and soil. 2003; 248: 43-59.

Gargouri K, Mhiri A. Relationship between soil fertility. Phosphorus and Potassium nutrition of the olive groves in Tunisia. Ed. I.N.A., Tunisia. 2002

Mouas Bourbia S, Barré P, Boudiaf Nait Kaci M, Derridj A, Velde V. Potassium status in bulk and rhizospheric soils of olive groves in north Algeria. Geoderma. 2013; 161-168.

Larif M, Zarrouk A, Soulaymani A, Elmidaoui A. New innovation in order to recover the polyphenols of olive mill wastewater extracts for use as a bio pesticide against the Euphylluraolivina and Aphis citricola. Res Chem Intermed. 2013; 39(9): 4303-4313.

Dibou A, Ksantini M, Raptopoulos D. Essais de l'efficacité du bio insecticide tétrastop en vue d'un contrí´le biologique du psylle de l'olivier Euphyllura olivina Costa (Homoptera, Psyllidae). Rev. Ezz. 2010; 11(1): 1-10.

Feeny P. Seasonal changes in Oak leaf Tannins and nutrients as a cause of spring feeding by winter moth caterpillars. Ecology. 1970; 51(4), 565-581.

Bryant JP, Chapin FS, Klein DR. Carbon/nutrient balance of Boreal plants in relation to vertebrate herbivory. OIKOS. 1983; 40, 357-368.

Ben Hamouda A, Boussadia O, Khaoula B, Laarif A, Braham M. Studies on insecticidal and deterrent effects of olive leaf extracts on Myzus persicae and Phthorimaea operculella. J EZS. 2015; 3(6): 294-297.

Ortega-García F, Peragón J. HPLC analysis of oleuropein, hydroxytyrosol, and tyrosol in stems and roots of Olea europaea L. cv. Picual during ripening. J Sci Food Agric. 2011; 90: 2295-2300.

Fernández-Bolaños JG, López í“, López-García Mí, Marset A. 2012. Biological Properties of Hydroxytyrosol and Its Derivatives. Olive Oil Constituents, Quality, Health Properties and Bioconversions, Dimitrios Boskou (Ed.), InTech. ISBN: 978-953-307-921-9: 375-396.

Al-Rimawi F. Development and validation of a simple reversedphase HPLC-UV method for determination of oleuropein in olive leaves. J Food Drug Anal. 2014; 22: 285-289.

Katsoulieris EN. The olive leaf extract oleuropein exerts protective effects against oxidant-induced cell death, concurrently displaying pro-oxidant activity in human hepatocarcinoma cells. Redox Rep. 2016; 21(2): 90-97.

Čepo DV, Albahari P, Konćić MZ, Radić K, Jurmanović S, Jug M. Solvent extraction and chromatographic determination of polyphenols in olive pomace. Food in Health and Disease. J Nutr Diet. 2017; (1) 7-14

Belaqziz M. Tan SP, El-Abbassi A, Kiai H, Hafidi A, O'Donovan O, McLoughlin P. Assessment of the antioxidant and antibacterial activities of different olive processing wastewaters. PLoS One. 2017; 12(9): 1-16.

Malik NSA, Joe M, Bradford JM. Changes in oleuropein levels during differentiation and development of floral buds in ‘Arbequina' olives. Sci Hortic. 2006; 110: 274–278

Koudounas K, Banilas G, Michaelidis Ch, Demoliou C, Rigas S, Hatzopoulos P. A defence-related Olea europaea Î²-glucosidase hydrolyses and activates oleuropein into a potent protein cross-linking agent. J Exp Bot. 2015; 66(7): 2093–2106.

Konno K, Hirayama C, Yasui H, Nakamura M. Enzymatic activation of oleuropein: a protein crosslinker used as a chemical defence in the privet tree. Pro Natl Acad Sci. 1999; 96: 9159–9164.

Hassan A. Effects of mineral nutrients on physiological and biochemical processes related to secondary metabolites production in medicinal herbs. Medicinal and Aromatic Plant Sci and Biotech. 2012; 6(1), 105-110.

Duarte AR, Santos SC, Seraphin JC, Ferri PH. Influence of spatial, edaphic and genetic factors on phenols and essential oils of Myrciaria cauliflora fruits. J. Braz. Chem. Soc. 2012; 23(4): 737-746.

Orians CM, Lower S, Fritz RS, Roche BM. The effects of plant genetic variation and soil nutrients on secondary chemistry and growth in shrubby willow, Salix sericea : patterns and constraints on the evolution of resistance traits. Biochem Syst Ecol. 2003; 31: 233-247.

Di Ferdinando M, Brunetti C, Agati G, Tattini M. Multiple functions of polyphénols in plants inhabiting unfavorable Mediterranean areas. Env Exp Bota. 2014; 103: 107-116.

Radix P, Bastien C, Jay C, Charlot G, Seigle F. The influence of soil nature on polyphenols in walnut tissues. A possible explanation of differences in the expression of walnut blight. Inra/Elsevier, Paris. Agronomie. 1988; 18: 627- 637.

Estiarte M, Filella I, Serra J, Peñuelas J. Effects of nutrients and water stress on leaf phenolic content of peppers and susceptibility to generalist herbivore Helicoverpa armigera (Hubner). Springer-Verlag

Author Biographies

Nadjet G. Mestar, Laboratory of analytic biochemistry research and biotechnology (LABAB). Department of Biochemistry and Microbiology, Faculty of Biological Sciences and Agronomic Sciences. Mouloud Mammeri University, Tizi-Ouzou, 15000, Algeria

Départment of Biology

Malika N. Boudiaf, Laboratoire Resources Naturelles (LRN). Department of Agronomy, Faculty of Biological Sciences and Agronomic Sciences. Mouloud Mammeri University, Tizi-Ouzou, 15000, Algeria

Department od Agronomy

Souad Lahcene, Laboratory of analytic biochemistry research and biotechnology (LABAB). Department of Biochemistry and Microbiology, Faculty of Biological Sciences and Agronomic Sciences. Mouloud Mammeri University, Tizi-Ouzou, 15000, Algeria

Department of Biochemistry and Microbiology

Hocine Abbaci, Laboratory of Mastery of Renewable Energies, University of Bejaia, Targa Ouzemmour, 06000 Bejaia. Algeria

Biology

Ghenima I. Aiche, Laboratory of analytic biochemistry research and biotechnology (LABAB). Department of Biochemistry and Microbiology, Faculty of Biological Sciences and Agronomic Sciences. Mouloud Mammeri University, Tizi-Ouzou, 15000, Algeria

Department of Biochemistry and Microbiology

Boussad Metna, Laboratoire Resources Naturelles (LRN). Department of Agronomy, Faculty of Biological Sciences and Agronomic Sciences. Mouloud Mammeri University, Tizi-Ouzou, 15000, Algeria

Department of Agronomy

Noria S. Saadoun, Laboratoire Resources Naturelles (LRN). Department of Agronomy, Faculty of Biological Sciences and Agronomic Sciences. Mouloud Mammeri University, Tizi-Ouzou, 15000, Algeria

Department of Biology

Faiza Taibi, Research Laboratory on the Biodiversity and Ecosystem pollution. Department of Agronomy, Nature and Life Sciences Faculty, University Chadli Bendjedid El-Tarf, 36000, Algeria

Department of Biology