Effect of GDF11 on proliferation and apoptosis of esophageal cancer cells

Biao Gu; Qi Wang; Weijie Dai; Gang Ma; Yaqi Song; Qingquan Wu

doi:10.14715/cmb/2018.64.11.14

Issue

Vol. 64 No. 11: Issue 11

Issue Published : August 31, 2018

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Effect of GDF11 on proliferation and apoptosis of esophageal cancer cells

https://doi.org/10.14715/cmb/2018.64.11.14

Biao Gu

Department of Thoracic Surgery, The Affiliated Huai'an No.1 People's Hospital of Nanjing Medical University, 6 Beijing West Road, Huai'an, Jiangsu 223300, China

Qi Wang

Department of Thoracic Surgery, The Affiliated Huai'an No.1 People's Hospital of Nanjing Medical University, 6 Beijing West Road, Huai'an, Jiangsu 223300, China

Weijie Dai

Department of Gastroenterology, The Affiliated Huai'an No.1 People's Hospital of Nanjing Medical University, 6 Beijing West Road, Huai'an, Jiangsu 223300, China

Gang Ma

Department of Gastroenterology, The Affiliated Huai'an No.1 People's Hospital of Nanjing Medical University, 6 Beijing West Road, Huai'an, Jiangsu 223300, China

Yaqi Song

Department of radiation oncology, The Affiliated Huai'an No.1 People's Hospital of Nanjing Medical University, 6 Beijing West Road, Huai'an, Jiangsu 223300, China

Qingquan Wu

Department of Thoracic Surgery, The Affiliated Huai'an No.1 People's Hospital of Nanjing Medical University, 6 Beijing West Road, Huai'an, Jiangsu 223300, China

Corresponding Author(s) : Biao Gu

xm1279@163.com

Cellular and Molecular Biology, Vol. 64 No. 11: Issue 11
Article Published : August 30, 2018

Abstract

This study was aimed at investigating the effect of growth differentiation factor 11 (GDF11) on the proliferation and apoptosis of esophageal cancer cells. Serum levels of GDF11 in esophageal cancer patients were determined with ELISA kits, and the correlation between serum GDF11 and pathological features of esophageal cancer were determined. The effect of recombinant GDF11 on the growth of esophageal cancer cells was measured by CCK6 method. In order to investigate the effect of recombinant GDF11 on the proliferation and apoptosis of esophageal cancer cells, the expression of apoptosis-promoting protein Bax and proliferative-associated protein Bcl-2 in esophageal cancer cells were determined using western blot. Moreover, GDF11 was used to treat esophageal cancer cells, and its effect on proliferation and apoptosis was determined with MTT assay and flow cytometry, respectively. The serum content of GDF11 was much less in esophageal cancer patients than in the control group. Esophageal GDF II in cancer patients was correlated with cancer differentiation: the higher the degree of differentiation, the higher the content of GDF11. GDF11 inhibits proliferation and apoptosis of esophageal cancer cells.

Keywords

Growth differentiation factor 11 Esophageal cancer Clinico-pathological features Apoptosis.

Gu, B., Wang, Q., Dai, W., Ma, G., Song, Y., & Wu, Q. (2018). Effect of GDF11 on proliferation and apoptosis of esophageal cancer cells. Cellular and Molecular Biology, 64(11), 80–84. https://doi.org/10.14715/cmb/2018.64.11.14

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References

Luo J, Wang W, Tang Y, Zhou D, Gao Y, Zhang Q, et al. mRNA and methylation profiling of radioresistant esophageal cancer cells: the involvement of Sall2 in acquired aggressive phenotypes. J Cancer 2017; 8: 646.
Shridhar R, Almhanna K, Meredith KL, Biagioli MC, Chuong MD, Cruz A, et al. Cancer Control 2013; 20: 97-110.
Tewari A. A cranberry proanthocyanidin inhibits cancer-related processes in human esophageal adenocarcinoma cells. Ohio State Univ 2006; 13: 51-55.
Kaur BS, Khamnehei N, Iravani M, Namburu SS, Lin O, Triadafilopoulos G. Rofecoxib inhibits cyclooxygenase 2 expression and activity and reduces cell proliferation in Barrett's esophagus. Gastroenterol 2002; 123: 60-67.
Cohen E, Bolus R, Khanna D, Hays RD, Chang L, Melmed GY, et al. GERD symptoms in the general population: prevalence and severity versus care-seeking patients. Dig Dis Sci 2014; 59: 2488-2496.
Zhang HY, Spechler SJ, Souza RF. Esophageal Adenocarcinoma Arising in Barrett Esophagus. Cancer Lett 2009; 275: 170-177.
Khavinson VK, Kuznik BI, Tarnovskaya SI, Linkova NS. GDF11 protein as a geroprotector. Biol Bull Rev 2016; 6: 141-148.
Andersson O, Reissmann E, Ibáñez CF. Growth differentiation factor 11 signals through the transforming growth factor-Î² receptor ALK5 to regionalize the anterior–posterior axis. Embo Rep 2006; 7: 831-837.
Bulterijs S. Metformin as a geroprotector. Rejuvenation Res 2011; 14: 469-482.
Bitto A, Kaeberlein M. Rejuvenation: it's in our blood. Cell Metabol 2014; 20: 2-4.
Sinha M, Jang YC, Oh J, Khong D, Wu EY, Manohar R, et al. Restoring systemic GDF11 levels reverses age-related dysfunction in mouse skeletal muscle. Sci 2014; 344: 649.
Lambert R, Hainaut P. The multidisciplinary management of gastrointestinal cancer. Epidemiology of oesophagogastric cancer. Best Pract Res Clin Gastroenterol 2007; 21: 921-945.
Herszényi L, Tulassay Z. Epidemiology of gastrointestinal and liver tumors. Eur Rev Med Pharmacol Sci 2010; 14: 249-258.
Chin H, Miller M, Kong CY, Emily CD, Kevin JN, Michelle D, et al. Trends in esophageal adenocarcinoma incidence and mortality. Cancer 2013; 119: 1149-1158.
Allen DC. Oesophageal Carcinoma. S Afr Med J 1973; 47: 348.
Harmon EB, Apelqvist AA, Smart NG, Gu X, Osborne DH, Kim SK. GDF11 modulates NGN3+ islet progenitor cell number and promotes beta-cell differentiation in pancreas development. Dev 2004; 131: 6163.
Ge G, Hopkins DR, Ho WB, Greenspan DS. GDF11 Forms a Bone Morphogenetic Protein 1-Activated Latent Complex That Can Modulate Nerve Growth Factor-Induced Differentiation of PC12 Cells. Mol Cell Biol 2005; 25: 5846-5858.
Liu JP, Laufer E, Jessell TM. Assigning the positional identity of spinal motor neurons: rostrocaudal patterning of Hox-c expression by FGFs, Gdf11, and retinoids. Neuron 2001; 32: 997-1012.
Shamsian BS, Arzanian MT, Shamshiri AR, Alavi S. Frequency of Red Cell Alloimmunization in Patients with Î²-Major Thalassemia in an Iranian Referral Hospital. Iran J Pediatr 2008; 18: 149-153.
Dussiot M, Maciel TT, Fricot A, Chartier C, Negre O, Veiga J, et al. An activin receptor IIA ligand trap corrects ineffective erythropoiesis in Î²-thalassemia. Nat Med 2014; 20: 398-407.
Rochette L, Zeller M, Cottin Y, et al. Growth and differentiation factor 11 (GDF11): Functions in the regulation of erythropoiesis and cardiac regeneration. Pharmacol Ther 2015; 156: 26-33.
Anisimov VN, Popovich IG, Zabezhinski MA, Anisimov SV, Vesnushkin GM, Vinogradova IA. Melatonin as antioxidant, geroprotector and anticarcinogen. Biochim Biophys Acta 2006; 1757: 573-589.
Williams G, Zentar MP, Gajendra S, Sonego M, Doherty P, Lalli G. Transcriptional Basis for the Inhibition of Neural Stem Cell Proliferation and Migration by the TGFÎ²-Family Member GDF11. Plos One 2013; 8: 78478.
Estrada E, Colón M, Báez P, Ruiz A, Seto E, Flores I, et al. Expression of the Cell Cycle Regulator GDF11 is Downregulated in Endometriosis. Soc Gynecol Investig 2011; 18: 190-191.
Zhang YH, Feng C, Du XT, Gao JLï¼ŒXiao XL, Li N, et al. GDF11/BMP11 activates both smad1/5/8 and smad2/3 signals but shows no significant effect on proliferation and migration of human umbil

References

Luo J, Wang W, Tang Y, Zhou D, Gao Y, Zhang Q, et al. mRNA and methylation profiling of radioresistant esophageal cancer cells: the involvement of Sall2 in acquired aggressive phenotypes. J Cancer 2017; 8: 646.

Shridhar R, Almhanna K, Meredith KL, Biagioli MC, Chuong MD, Cruz A, et al. Cancer Control 2013; 20: 97-110.

Tewari A. A cranberry proanthocyanidin inhibits cancer-related processes in human esophageal adenocarcinoma cells. Ohio State Univ 2006; 13: 51-55.

Kaur BS, Khamnehei N, Iravani M, Namburu SS, Lin O, Triadafilopoulos G. Rofecoxib inhibits cyclooxygenase 2 expression and activity and reduces cell proliferation in Barrett's esophagus. Gastroenterol 2002; 123: 60-67.

Cohen E, Bolus R, Khanna D, Hays RD, Chang L, Melmed GY, et al. GERD symptoms in the general population: prevalence and severity versus care-seeking patients. Dig Dis Sci 2014; 59: 2488-2496.

Zhang HY, Spechler SJ, Souza RF. Esophageal Adenocarcinoma Arising in Barrett Esophagus. Cancer Lett 2009; 275: 170-177.

Khavinson VK, Kuznik BI, Tarnovskaya SI, Linkova NS. GDF11 protein as a geroprotector. Biol Bull Rev 2016; 6: 141-148.

Andersson O, Reissmann E, Ibáñez CF. Growth differentiation factor 11 signals through the transforming growth factor-Î² receptor ALK5 to regionalize the anterior–posterior axis. Embo Rep 2006; 7: 831-837.

Bulterijs S. Metformin as a geroprotector. Rejuvenation Res 2011; 14: 469-482.

Bitto A, Kaeberlein M. Rejuvenation: it's in our blood. Cell Metabol 2014; 20: 2-4.

Sinha M, Jang YC, Oh J, Khong D, Wu EY, Manohar R, et al. Restoring systemic GDF11 levels reverses age-related dysfunction in mouse skeletal muscle. Sci 2014; 344: 649.

Lambert R, Hainaut P. The multidisciplinary management of gastrointestinal cancer. Epidemiology of oesophagogastric cancer. Best Pract Res Clin Gastroenterol 2007; 21: 921-945.

Herszényi L, Tulassay Z. Epidemiology of gastrointestinal and liver tumors. Eur Rev Med Pharmacol Sci 2010; 14: 249-258.

Chin H, Miller M, Kong CY, Emily CD, Kevin JN, Michelle D, et al. Trends in esophageal adenocarcinoma incidence and mortality. Cancer 2013; 119: 1149-1158.

Allen DC. Oesophageal Carcinoma. S Afr Med J 1973; 47: 348.

Harmon EB, Apelqvist AA, Smart NG, Gu X, Osborne DH, Kim SK. GDF11 modulates NGN3+ islet progenitor cell number and promotes beta-cell differentiation in pancreas development. Dev 2004; 131: 6163.

Ge G, Hopkins DR, Ho WB, Greenspan DS. GDF11 Forms a Bone Morphogenetic Protein 1-Activated Latent Complex That Can Modulate Nerve Growth Factor-Induced Differentiation of PC12 Cells. Mol Cell Biol 2005; 25: 5846-5858.

Liu JP, Laufer E, Jessell TM. Assigning the positional identity of spinal motor neurons: rostrocaudal patterning of Hox-c expression by FGFs, Gdf11, and retinoids. Neuron 2001; 32: 997-1012.

Shamsian BS, Arzanian MT, Shamshiri AR, Alavi S. Frequency of Red Cell Alloimmunization in Patients with Î²-Major Thalassemia in an Iranian Referral Hospital. Iran J Pediatr 2008; 18: 149-153.

Dussiot M, Maciel TT, Fricot A, Chartier C, Negre O, Veiga J, et al. An activin receptor IIA ligand trap corrects ineffective erythropoiesis in Î²-thalassemia. Nat Med 2014; 20: 398-407.

Rochette L, Zeller M, Cottin Y, et al. Growth and differentiation factor 11 (GDF11): Functions in the regulation of erythropoiesis and cardiac regeneration. Pharmacol Ther 2015; 156: 26-33.

Anisimov VN, Popovich IG, Zabezhinski MA, Anisimov SV, Vesnushkin GM, Vinogradova IA. Melatonin as antioxidant, geroprotector and anticarcinogen. Biochim Biophys Acta 2006; 1757: 573-589.

Williams G, Zentar MP, Gajendra S, Sonego M, Doherty P, Lalli G. Transcriptional Basis for the Inhibition of Neural Stem Cell Proliferation and Migration by the TGFÎ²-Family Member GDF11. Plos One 2013; 8: 78478.

Estrada E, Colón M, Báez P, Ruiz A, Seto E, Flores I, et al. Expression of the Cell Cycle Regulator GDF11 is Downregulated in Endometriosis. Soc Gynecol Investig 2011; 18: 190-191.

Zhang YH, Feng C, Du XT, Gao JLï¼ŒXiao XL, Li N, et al. GDF11/BMP11 activates both smad1/5/8 and smad2/3 signals but shows no significant effect on proliferation and migration of human umbil

Author biographies is not available.