Effects of different concentrations of mannitol on gene expression in Stevia rebaudiana Bertoni

Somaye Hashempoor; Matin Ghaheri; Danial Kahrizi; Nastaran Kazemi; Saare Muhammadi; Seyed Mehdi Safavi; Tayebeh Ghorbani; Elham Rahmanian; Malihe Heshmatpanaah

doi:10.14715/cmb/2018.64.2.6

Issue

Vol. 64 No. 2: New biotechnological aspects in Stevia rebaudiana Bertoni

Issue Published : February 11, 2018

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Effects of different concentrations of mannitol on gene expression in Stevia rebaudiana Bertoni

https://doi.org/10.14715/cmb/2018.64.2.6

Somaye Hashempoor

Department of Medicinal Plants, Institute of Higher Education, Jahad-e-Daneshgahi, Kermanshah Unit, Iran

Matin Ghaheri

Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Danial Kahrizi

Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Nastaran Kazemi

Department of Medicinal Plants, Institute of Higher Education, Jahad-e-Daneshgahi, Kermanshah Unit, Iran

Saare Muhammadi

Department of Cardiology, Kermanshah University of Medical Sciences, Kermanshah, Iran

Seyed Mehdi Safavi

Department of Agronomy and Plant Breeding, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran

Tayebeh Ghorbani

Zagros Bioidea Company, Kermanshah, Iran

Elham Rahmanian

Department of Sexual Medicine; and The Rhazes Center for Research in Family Health and Sexual Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

Malihe Heshmatpanaah

Zagros Bioidea Company, Kermanshah, Iran

Corresponding Author(s) : Matin Ghaheri

matingh92@gmail.com

Cellular and Molecular Biology, Vol. 64 No. 2: New biotechnological aspects in Stevia rebaudiana Bertoni
Article Published : February 10, 2018

Abstract

Stevia rebaudiana Bertoni is one of the most important herbal sweetener plants from Asteracea family that have a lot of Steviol glycosides. Among different methods, tissue culture is the best way with high efficiency that is useful for studying stress tolerance mechanisms to obtain drought tolerance of stevia. For this purpose, different concentrations of mannitol (0, 10, 20, 30, 40, 50 mg/l) were used as various treatments in the culture medium of stevia. According to the results, the highest level of UGT85C2 gene expression (1.181 Total lab unit) was seen in plants grown under 30 mg/l mannitol treatment and the lowest level of this gene expression (0.603 Total lab unit) was observed under 40 mg/l mannitol treatment. However, the highest level of KO gene expression (1.323 Total lab unit) was observed under 20 mg/l mannitol. It shows stevia growth is affected by osmotic stress. Water deficiency has a negative impact on Stevia. However, the expression of genes had increased by particular mannitol concentrations. Actually, stevia can survive under various abiotic stresses.

Keywords

Stevia rebaudiana Bertoni Mannitol Semi-quantitative RT-PCR Tissue culture UGT85C2 KO.

Hashempoor, S., Ghaheri, M., Kahrizi, D., Kazemi, N., Muhammadi, S., Safavi, S. M., Ghorbani, T., Rahmanian, E., & Heshmatpanaah, M. (2018). Effects of different concentrations of mannitol on gene expression in Stevia rebaudiana Bertoni. Cellular and Molecular Biology, 64(2), 28–31. https://doi.org/10.14715/cmb/2018.64.2.6

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References

AL-Taha HAK. Effect of shock and gradual drought by PEG on callus growth and proline accumulation in sour orange (Citrus aurantium). Adv Agric Bot Int J Bio Soc 2013; 5(2):77-83.
Rai MK, Kalia RK, Singh R, Dhawan AK. Developing stress tolerant plants through in vitro selection an overview of therecent progress. Env Exp Bot 2011; 71:89-98.
Anbazhagan M, Kalpana M, Rajendran R, Natarajan V, Dhanavel D. In vitro production of Stevia rebaudiana Bertoni. Emir J food agric 2010; 22:216-222.
Singh S, Garg V, Yadav D, Beg MN, Sharma N. In-vitro antioxidative and antibacterial activities of various parts of stevia rebaudiana (Bertoni). Int J Pharm Pharm Sci 2012; 4(3):468-473.
Prakash I, Markosyan A, Bunders C. Development of Next Generation Stevia Sweetener: Rebaudioside M. Foods 2014; 3:162-175.
Mondaca RL, Glvez AV, Bravo LZ, Hen KA. Stevia rebaudiana Bertoni, source of a high-potency natural sweetener: A comprehensive review on the biochemical, nutritional and functional aspects. Food Chem 2012; 132:1121-1132.
Jitendra M, Monika S, Ratan SD, Priyanka G, Priyanka S, Kiran DJ. Micropropagation of an Anti diabetic Plant - Stevia rebaudiana Bertoni, (Natural Sweetener) in Hadoti Region of South-East Rajas¬than, India ISCA J Biol Sci 2012; 1:37-42.
Yadav P, Kumari P, Arya A, Tripathi S, Kumar S. Effect of ni¬trogen sources on rooting of in vitro culture of Stevia rebaudiana Bertoni. J Biotechnol 2013; 4:41-46.
Verma R, Gena DD, Lal Jat B. In vitro propagation of Stevia re¬baudiana Bertoni (a sweeting plant). J Pharm Res 2016; 5:666-685.
Fronza, D, Folegatti MV. Water consumption of the Stevia (Stevia rebaudiana Bert.) crop estimated through microlysimiter. Scientia Agricola 2003; 60-80.
Geuns JMC. Molecules of interest stevioside. Phytochemistry 2003; 6:913-921.
Gupta P, Sharma S, Saxena S. Effect of Salts (NaCl and Na2CO3) on Callus and Suspension Culture of Stevia rebaudiana for Steviol Glycoside Production. Appl Biochem Biotechnol 2014; 172:2894-2906.
Zeng J, Chen A, Li D, Yi B, Wu W. Effects of salt stress on the growth, physiological responses, and glycoside contents of Stevia rebaudiana Bertoni. J Agric Food Chem 2013; 61(24):5720-6.
Abdul Razak UNA, Ong CB, Yu TS, Lau LK. In vitro Micropropagation of Stevia rebaudiana Bertoni in Malaysia. Braz Arch Biol Technol 2014; 1:23-28.
Raina R, Bhandari SK, Chand R, Sharma Y. Strategies to improve poor seed germination in Stevia rebaudiana, a low calorie sweetener. J Med Plants Res 2013; 7:1793-1799.
Savita S, Sheela K, Sunanda S, Shankar A, Ramakrishna P. Stevia rebaudiana a functional component for food industry. J Hum Ecol 2004; 15:261–264.
Kamran Khan M, Misra P, Sharma P, Shukla PK, Ramteke PW. Effect of adenine sulphate on 6. in vitro mass propagation of Stevia rebaudiana Bertoni. J Med Plants Res 2014; 8:543-549.
Mukundan U, Sivaram L. In vitro culture studies on Stevia rebaudiana. J In Vitro Cell Dev Biol 2003; 5:520-523
Brandle J, Telmer P. Steviol glycoside biosynthesis. Phytochemistry 2007; 68:1855-1863.
Richman A, Swanson A, Humphrey T, Chapman R, McGarvey B, Pocs R, et al. Functional genomics uncovers three glucosyltransferases involved in the synthesis of the major sweet glucosides of Stevia rebaudiana. Plant J 2005; 41:56-67.
Pandey H, Pandey P, Pandey Sh, Singh S, Banerjee S. Meeting the challenge of stevioside production in the hairy roots of Stevia rebaudiana by probing the underlying process. Plant Cell Tiss Organ Cult 2016; 126:511–52.
Ghaheri M, Kahrizi D, Bahrami G. Effect of mannitol on some morphological characteristics of in vitro stevia rebaudiana Bertoni. Biharean Biologist 2017; 11(2): (online first)
Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 1962; 15:473–479.
Marone M, Mozzetti S, Ritis DD, Pierelli L, Scambia G. Semiquantitative RT-PCR analysis to assess the expression levels of multiple transcripts from the same sample. Biol Proced Online 2001; 3(1):19-25.
Pandey M, Chikara SK. Effect of salinity and drought stress on growth parameters, glycoside content and expression level of vital genes in steviol glycosides biosynthesis pathway of Stevia rebaudiana (Bertoni). Int J Genet. 2015; 7(1), 153-160.
Kumar H, Kaul K, Bajpai-Gupta S, Kumar Kaul V, Kumar V. A comprehensive analysis of fifteen genes of steviol glycosides biosynthesis pathway in Stevia rebaudiana (Bertoni). Gene 2012; 492: 276–284.

References

AL-Taha HAK. Effect of shock and gradual drought by PEG on callus growth and proline accumulation in sour orange (Citrus aurantium). Adv Agric Bot Int J Bio Soc 2013; 5(2):77-83.

Rai MK, Kalia RK, Singh R, Dhawan AK. Developing stress tolerant plants through in vitro selection an overview of therecent progress. Env Exp Bot 2011; 71:89-98.

Anbazhagan M, Kalpana M, Rajendran R, Natarajan V, Dhanavel D. In vitro production of Stevia rebaudiana Bertoni. Emir J food agric 2010; 22:216-222.

Singh S, Garg V, Yadav D, Beg MN, Sharma N. In-vitro antioxidative and antibacterial activities of various parts of stevia rebaudiana (Bertoni). Int J Pharm Pharm Sci 2012; 4(3):468-473.

Prakash I, Markosyan A, Bunders C. Development of Next Generation Stevia Sweetener: Rebaudioside M. Foods 2014; 3:162-175.

Mondaca RL, Glvez AV, Bravo LZ, Hen KA. Stevia rebaudiana Bertoni, source of a high-potency natural sweetener: A comprehensive review on the biochemical, nutritional and functional aspects. Food Chem 2012; 132:1121-1132.

Jitendra M, Monika S, Ratan SD, Priyanka G, Priyanka S, Kiran DJ. Micropropagation of an Anti diabetic Plant - Stevia rebaudiana Bertoni, (Natural Sweetener) in Hadoti Region of South-East Rajas¬than, India ISCA J Biol Sci 2012; 1:37-42.

Yadav P, Kumari P, Arya A, Tripathi S, Kumar S. Effect of ni¬trogen sources on rooting of in vitro culture of Stevia rebaudiana Bertoni. J Biotechnol 2013; 4:41-46.

Verma R, Gena DD, Lal Jat B. In vitro propagation of Stevia re¬baudiana Bertoni (a sweeting plant). J Pharm Res 2016; 5:666-685.

Fronza, D, Folegatti MV. Water consumption of the Stevia (Stevia rebaudiana Bert.) crop estimated through microlysimiter. Scientia Agricola 2003; 60-80.

Geuns JMC. Molecules of interest stevioside. Phytochemistry 2003; 6:913-921.

Gupta P, Sharma S, Saxena S. Effect of Salts (NaCl and Na2CO3) on Callus and Suspension Culture of Stevia rebaudiana for Steviol Glycoside Production. Appl Biochem Biotechnol 2014; 172:2894-2906.

Zeng J, Chen A, Li D, Yi B, Wu W. Effects of salt stress on the growth, physiological responses, and glycoside contents of Stevia rebaudiana Bertoni. J Agric Food Chem 2013; 61(24):5720-6.

Abdul Razak UNA, Ong CB, Yu TS, Lau LK. In vitro Micropropagation of Stevia rebaudiana Bertoni in Malaysia. Braz Arch Biol Technol 2014; 1:23-28.

Raina R, Bhandari SK, Chand R, Sharma Y. Strategies to improve poor seed germination in Stevia rebaudiana, a low calorie sweetener. J Med Plants Res 2013; 7:1793-1799.

Savita S, Sheela K, Sunanda S, Shankar A, Ramakrishna P. Stevia rebaudiana a functional component for food industry. J Hum Ecol 2004; 15:261–264.

Kamran Khan M, Misra P, Sharma P, Shukla PK, Ramteke PW. Effect of adenine sulphate on 6. in vitro mass propagation of Stevia rebaudiana Bertoni. J Med Plants Res 2014; 8:543-549.

Mukundan U, Sivaram L. In vitro culture studies on Stevia rebaudiana. J In Vitro Cell Dev Biol 2003; 5:520-523

Brandle J, Telmer P. Steviol glycoside biosynthesis. Phytochemistry 2007; 68:1855-1863.

Richman A, Swanson A, Humphrey T, Chapman R, McGarvey B, Pocs R, et al. Functional genomics uncovers three glucosyltransferases involved in the synthesis of the major sweet glucosides of Stevia rebaudiana. Plant J 2005; 41:56-67.

Pandey H, Pandey P, Pandey Sh, Singh S, Banerjee S. Meeting the challenge of stevioside production in the hairy roots of Stevia rebaudiana by probing the underlying process. Plant Cell Tiss Organ Cult 2016; 126:511–52.

Ghaheri M, Kahrizi D, Bahrami G. Effect of mannitol on some morphological characteristics of in vitro stevia rebaudiana Bertoni. Biharean Biologist 2017; 11(2): (online first)

Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 1962; 15:473–479.

Marone M, Mozzetti S, Ritis DD, Pierelli L, Scambia G. Semiquantitative RT-PCR analysis to assess the expression levels of multiple transcripts from the same sample. Biol Proced Online 2001; 3(1):19-25.

Pandey M, Chikara SK. Effect of salinity and drought stress on growth parameters, glycoside content and expression level of vital genes in steviol glycosides biosynthesis pathway of Stevia rebaudiana (Bertoni). Int J Genet. 2015; 7(1), 153-160.

Kumar H, Kaul K, Bajpai-Gupta S, Kumar Kaul V, Kumar V. A comprehensive analysis of fifteen genes of steviol glycosides biosynthesis pathway in Stevia rebaudiana (Bertoni). Gene 2012; 492: 276–284.

Author biographies is not available.