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Vol. 64 No. 12: Issue 12

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A simplified and optimized protocol for total DNA extraction from insect species: applicable for studying genetic diversity and PCR-based specimen identification via partial amplification of cytochrome oxidase I (COI) gene

https://doi.org/10.14715/cmb/2018.64.12.5
Samira Paydar
Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran
Esra Ucar
Department of Crop and Animal Production, Sivas Vocational School, Cumhuriyet University, Sivas, Turkey
Peyman Yari
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
Seyed Mehdi Safavi
Department of Agronomy and Plant Breeding, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran
Danial Kahrizi
Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
Masoud Nateqi
Department of Plant Protection, Faculty of Agricultural Sciences and Engineering, University of Tehran, Karaj, Iran
Alinaghi Mirmoayedi
Department of Plant Protection, Razi University, Kermanshah, Iran
Kheirollah Yari
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Corresponding Author(s) : Kheirollah Yari

khirollah.yari@yahoo.com

Cellular and Molecular Biology, Vol. 64 No. 12: Issue 12

Abstract

The efficient DNA extraction from insects has been suggested as a critical and main step affecting molecular entomology for taxonomic identification, the establishment of DNA barcoding library and analysis of genetic diversity relationship between insect populations. For successfully apply these molecular techniques, high-quantity and high-quality of the extracted DNA are required. Several protocols for efficient genomic DNA extraction from insects have been developed. In this research, we represent a rapid, reliable and cost-effective method that it is not reliant on poisonous and enzymatic reagents for DNA extraction from insect tissues. Results showed that high quantity and high-quality of the isolated DNA by this method is suitable and can be used directly for PCR, also is enough to do hundreds of molecular reactions. In conclusion, we described a fast, cost-effective, non-toxic and enzyme-free protocol for high yield genomic DNA extraction from green Lacewings (Chrysoperla carnea) tissues in basic equipment laboratories.

Keywords

DNA extraction method Optimized protocol Chrysopidae Cytochrome oxidase I.
Paydar, S., Ucar, E., Yari, P., Safavi, S. M., Kahrizi, D., Nateqi, M., Mirmoayedi, A., & Yari, K. (2018). A simplified and optimized protocol for total DNA extraction from insect species: applicable for studying genetic diversity and PCR-based specimen identification via partial amplification of cytochrome oxidase I (COI) gene. Cellular and Molecular Biology, 64(12), 22–25. https://doi.org/10.14715/cmb/2018.64.12.5
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