Effects of grape seed extract, quercetin and vitamin C on ovine oocyte maturation and subsequent embryonic development

Maryam Karimian; Mohammad Zandi; Mohammad Reza Sanjabi; Majid Masoumian; Hamideh Ofoghi

doi:10.14715/cmb/2018.64.4.16

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Vol. 64 No. 4: Issue 4

Issue Published : April 2, 2018

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Effects of grape seed extract, quercetin and vitamin C on ovine oocyte maturation and subsequent embryonic development

https://doi.org/10.14715/cmb/2018.64.4.16

Maryam Karimian

Department of Agriculture, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran

Mohammad Zandi

Department of Agriculture, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran

Mohammad Reza Sanjabi

Department of Agriculture, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran

Majid Masoumian

Department of Agriculture, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran

Hamideh Ofoghi

Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran

Corresponding Author(s) : Mohammad Zandi

mz1075@yahoo.com

Cellular and Molecular Biology, Vol. 64 No. 4: Issue 4
Article Published : March 31, 2018

Abstract

The present study aimed to evaluate the effects of grape seed extract (GSE) versus quercetin and vitamin C on in vitro oocyte maturation and embryo development in sheep. The free radical scavenging activity of different concentrations of each product was measured by 1, 1- diphenyl-2-picryl hydrazyl (DPPH). Oocytes were collected from ovaries of slaughtered ewes and matured in TCM-199 medium containing fetal calf serum, follicle stimulating hormone (FSH), estradiol-17 Î², sodium pyruvate, and gentamicin sulfate. The in vitro fertilization and culture were performed using Bracket and Oliphant's (BO) medium and modified Charles Rosenkrans medium with amino acids (mCR2aa), respectively. The results showed that the hydroalcoholic extract of grape seed had free radical scavenging activity. IC50 value for GSE, vitamin C, and quercetin was found to be 585 µg/mL, 53 µg/mL, and 43 µg/mL, respectively. The concentrations, which showed beneficial effects on oocyte maturation and early development based on the mean number of cleavage, morula and blastocyst rates, were 25-200 µg/mL, 5 or 15 µg/mL, and 800 µg/mL, respectively, for vitamin C, quercetin and GSE. However, there were no significant differences between different concentrations of GSE and control. Findings also highlight the great effect on blastocyst rate while adding GSE at 800 µg/mL. However, the best rate of blastocyst production was obtained in presence of quercetin. Findings suggested the need for further studies on special molecules derived from GSE.

Keywords

Antioxidant Blastocyst Grape seed extract Quercetin Vitamin C Sheep.

Karimian, M., Zandi, M., Sanjabi, M. R., Masoumian, M., & Ofoghi, H. (2018). Effects of grape seed extract, quercetin and vitamin C on ovine oocyte maturation and subsequent embryonic development. Cellular and Molecular Biology, 64(4), 98–102. https://doi.org/10.14715/cmb/2018.64.4.16

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References

Soï¬ KA, Khan MZ, Islam R, Lone FA. Effect of cysteamine and epidermal growth factor supplementation on the in vitro maturation rate of ovine oocytes. Small Rum Res 2011; 96:191-194.
Jeon Y, Yoon JD, Cai L, Hwang S, Kim E, Zheng Z, Lee E, Kim DY, Hyun S. Supplementation of zinc on oocyte in vitro maturation improves preimplatation embryonic development in pigs. Theriogenology 2014; 82:866-874.
Maleki EM, Eimani H, Bigdeli MR, Ebrahimi B, Shahverdi AH, Narenji AG, Abedi R. A comparative study of saffron aqueous extract and its active ingredient, crocin on the in vitro maturation, in vitro fertilization, and in vitro culture of mouse oocytes. Taiwan J Obstet Gynecol 2014; 53:21-25.
Kang J, Moon JH, Choi J, Park SJ, Kim SJ, Saadeldin IM, Lee BC. Effect of antioxidant flavonoids (Quercetin and Taxifolin) on in vitro maturation of porcine oocytes. Asian Australas. J Anim Sci 2016; 29:352-358.
Rajabi-Toustani R, Motamedi-Mojdehi R, Roostaei-Ali Mehr M, Motamedi-Mojdehi R. Effect of Papaver rhoeas L. extract on in vitro maturation of sheep oocytes. Small Rum Res 2013; 114:146–151.
Yu S, Long H, Lyu Q, Zhang Q, Yan Z, Liang H, Chai W, Yan Z, Kuang Y, Qi C. Protective effect of quercetin on the development of preimplantation mouse embryos against hydrogen peroxide-induced oxidative injury. Plos one 2014; 9:1-9.
Ky I, Teissedre P. Characterisation of mediterranean grape pomace seed and skin extracts: polyphenolic content and antioxidant activity. Molecules 2015; 20: 2190-2207.
Kalfin R, Righi A, Rosso AD, Bagchi D, Generini S, Guiducci S, Cerinic MM, Das D. Activin, a grape seed-derived proanthocyanidin extract, reduces plasma levels of oxidative stress and adhesion molecules (ICAM-1, VCAM-1 and E-selectin) in systemic sclerosis. Free Radic Res 2002; 36:819-825.
Samavardhana K, Supawititpattana P, Jittrepotch N, Rojsuntornkitti K, Kongbangkerd T. Effects of extracting conditions on phenolic compounds and antioxidant activity from different grape processing byproducts. Int Food Res J 2015; 22:1169-1179.
Lakshmi BVS, Sudhakar M, Shashank P. Protective role of hydroalcoholic extract of vitis vinifera against adriamycin induced cardiac, renal and hepatic toxicities in rat. Int J Pharm Sci Res Rev 2014; 3:13-19.
Orlovschi D, Miclea I, Zahan M, Miclea V, Pernes A. Quercetin efficacy on in vitro maturation of porcine oocytes. Anim Sci Biotech 2014;47:113-115.
Kang J, Kwon D, Park S, Kim S, Moon J, Koo O, Jang G, Lee B. Quercetin improves the in vitro development of porcine oocytes by decreasing reactive oxygen species levels. J Vet Sci 2013; 14:15-20.
Crozier A, Jensen E, Lean MEJ, McDonald MS. Quantitative analysis of flavonoids by reversed-phase high performance liquid chromatography. J of Chromatography A 1997;761:315-321.
Shekhar TC, Anju G. Antioxidant activity by DPPH radical scavenging method of Ageratum conyzoides Linn. leaves. Am J Ethnomed 2014;1:244-249.
Andzi-Barhé T, Massala KK, Engonga LCO, Lebibi J. Phytochemical studies, total phenolic and flavonoids content and evaluation of antiradical activity of the extracts of the leaves from Dischistocalyx sp. (Acanthacées). J Pharmacogn Phytochem 2015; 3:174-178.
Garde J, Aguado M, Perez S, Garrido D, Pérez-Guzmán M, Montoro, V. Physiological characteristics of epididymal spermatozoa from postmortem rams. Theriogenology 2003; 41: 203.
Hasanzadeh R, Zandi M, Sanjabi MR, Hoseini Pajooh K, Shabani H. Expression of fluorescent reporter protein was not obtained in ovine embryos produced through in vitro fertilization-sperm mediated gene transfer (IVF-SMGT). Small Rum Res 2016; 143:24-28.
Chauhan MS, Palta P, Das SK, Katiyar PK, Madan ML. Replacement of serum and hormone additives with follicular fluid in the IVM medium: effects on maturation, fertilization and subsequent development of buffalo oocytes in vitro. Theriogenology 1997; 48:461-469.
Kere M, Siriboon C, Lo N, Ju J. Ascorbic acid improves the developmental competence of porcine oocytes after parthenogenetic activation and somatic cell nuclear transplantation. Eur J Appl Sci 2012; 4:182-190.
Kere M, Siriboon C, Lo N, Nguyen NT, Ju J. Ascorbic acid improves the developmental competence of porcine oocytes after parthenogenetic activation and somatic cell nuclear transplantation. J Reprod Dev 2013; 59:78-84.
Naruse K, Kim HR, Shin YM, Chang SM, Lee HR, Park CS, Jin DL. Low concentrations of MEM vitamins during in vitro maturation of porcine oocytes improves subsequent parthenogenetic development. Theriogenology 2007; 67:407–412.
Dalvit G, Lianes SP, Descalzo A, Insani M, Beconi M, Cetica P. Effect of alpha-tocopherol and ascorbic acid on bovine oocyte in vitro maturation. Rep Dom Anim 2005; 40:93-97.
Wang ZG, Yu SD, Xu ZR. Improvement in bovine embryo production in vitro by treatment with green tea polyphenols during in vitro maturation of oocytes. Anim Reprod Sci 2007; 100:22-31.
Tavana S, Eimani H, Azarnia M, Shahverdi A, Eftekhari-Yazdi P. Effects of Saffron (Crocus sativus L.) aqueous extract on in vitro maturation fertilization and embryo development of mouse oocytes Cell J (Yakhteh) 2012; 13:259-264.
Golkar-Narenji A, Eimani H, Samadi F, Hasani S, Shahverdi AH, Eftekhari-Yazdi P, Kamalinejad M. Effect of Papaver rhoeas extract on in vitro maturation and developmental competence of immature mouse oocytes. Reprod Med Biol 2010; 9:211-215.

References

Soï¬ KA, Khan MZ, Islam R, Lone FA. Effect of cysteamine and epidermal growth factor supplementation on the in vitro maturation rate of ovine oocytes. Small Rum Res 2011; 96:191-194.

Jeon Y, Yoon JD, Cai L, Hwang S, Kim E, Zheng Z, Lee E, Kim DY, Hyun S. Supplementation of zinc on oocyte in vitro maturation improves preimplatation embryonic development in pigs. Theriogenology 2014; 82:866-874.

Maleki EM, Eimani H, Bigdeli MR, Ebrahimi B, Shahverdi AH, Narenji AG, Abedi R. A comparative study of saffron aqueous extract and its active ingredient, crocin on the in vitro maturation, in vitro fertilization, and in vitro culture of mouse oocytes. Taiwan J Obstet Gynecol 2014; 53:21-25.

Kang J, Moon JH, Choi J, Park SJ, Kim SJ, Saadeldin IM, Lee BC. Effect of antioxidant flavonoids (Quercetin and Taxifolin) on in vitro maturation of porcine oocytes. Asian Australas. J Anim Sci 2016; 29:352-358.

Rajabi-Toustani R, Motamedi-Mojdehi R, Roostaei-Ali Mehr M, Motamedi-Mojdehi R. Effect of Papaver rhoeas L. extract on in vitro maturation of sheep oocytes. Small Rum Res 2013; 114:146–151.

Yu S, Long H, Lyu Q, Zhang Q, Yan Z, Liang H, Chai W, Yan Z, Kuang Y, Qi C. Protective effect of quercetin on the development of preimplantation mouse embryos against hydrogen peroxide-induced oxidative injury. Plos one 2014; 9:1-9.

Ky I, Teissedre P. Characterisation of mediterranean grape pomace seed and skin extracts: polyphenolic content and antioxidant activity. Molecules 2015; 20: 2190-2207.

Kalfin R, Righi A, Rosso AD, Bagchi D, Generini S, Guiducci S, Cerinic MM, Das D. Activin, a grape seed-derived proanthocyanidin extract, reduces plasma levels of oxidative stress and adhesion molecules (ICAM-1, VCAM-1 and E-selectin) in systemic sclerosis. Free Radic Res 2002; 36:819-825.

Samavardhana K, Supawititpattana P, Jittrepotch N, Rojsuntornkitti K, Kongbangkerd T. Effects of extracting conditions on phenolic compounds and antioxidant activity from different grape processing byproducts. Int Food Res J 2015; 22:1169-1179.

Lakshmi BVS, Sudhakar M, Shashank P. Protective role of hydroalcoholic extract of vitis vinifera against adriamycin induced cardiac, renal and hepatic toxicities in rat. Int J Pharm Sci Res Rev 2014; 3:13-19.

Orlovschi D, Miclea I, Zahan M, Miclea V, Pernes A. Quercetin efficacy on in vitro maturation of porcine oocytes. Anim Sci Biotech 2014;47:113-115.

Kang J, Kwon D, Park S, Kim S, Moon J, Koo O, Jang G, Lee B. Quercetin improves the in vitro development of porcine oocytes by decreasing reactive oxygen species levels. J Vet Sci 2013; 14:15-20.

Crozier A, Jensen E, Lean MEJ, McDonald MS. Quantitative analysis of flavonoids by reversed-phase high performance liquid chromatography. J of Chromatography A 1997;761:315-321.

Shekhar TC, Anju G. Antioxidant activity by DPPH radical scavenging method of Ageratum conyzoides Linn. leaves. Am J Ethnomed 2014;1:244-249.

Andzi-Barhé T, Massala KK, Engonga LCO, Lebibi J. Phytochemical studies, total phenolic and flavonoids content and evaluation of antiradical activity of the extracts of the leaves from Dischistocalyx sp. (Acanthacées). J Pharmacogn Phytochem 2015; 3:174-178.

Garde J, Aguado M, Perez S, Garrido D, Pérez-Guzmán M, Montoro, V. Physiological characteristics of epididymal spermatozoa from postmortem rams. Theriogenology 2003; 41: 203.

Hasanzadeh R, Zandi M, Sanjabi MR, Hoseini Pajooh K, Shabani H. Expression of fluorescent reporter protein was not obtained in ovine embryos produced through in vitro fertilization-sperm mediated gene transfer (IVF-SMGT). Small Rum Res 2016; 143:24-28.

Chauhan MS, Palta P, Das SK, Katiyar PK, Madan ML. Replacement of serum and hormone additives with follicular fluid in the IVM medium: effects on maturation, fertilization and subsequent development of buffalo oocytes in vitro. Theriogenology 1997; 48:461-469.

Kere M, Siriboon C, Lo N, Ju J. Ascorbic acid improves the developmental competence of porcine oocytes after parthenogenetic activation and somatic cell nuclear transplantation. Eur J Appl Sci 2012; 4:182-190.

Kere M, Siriboon C, Lo N, Nguyen NT, Ju J. Ascorbic acid improves the developmental competence of porcine oocytes after parthenogenetic activation and somatic cell nuclear transplantation. J Reprod Dev 2013; 59:78-84.

Naruse K, Kim HR, Shin YM, Chang SM, Lee HR, Park CS, Jin DL. Low concentrations of MEM vitamins during in vitro maturation of porcine oocytes improves subsequent parthenogenetic development. Theriogenology 2007; 67:407–412.

Dalvit G, Lianes SP, Descalzo A, Insani M, Beconi M, Cetica P. Effect of alpha-tocopherol and ascorbic acid on bovine oocyte in vitro maturation. Rep Dom Anim 2005; 40:93-97.

Wang ZG, Yu SD, Xu ZR. Improvement in bovine embryo production in vitro by treatment with green tea polyphenols during in vitro maturation of oocytes. Anim Reprod Sci 2007; 100:22-31.

Tavana S, Eimani H, Azarnia M, Shahverdi A, Eftekhari-Yazdi P. Effects of Saffron (Crocus sativus L.) aqueous extract on in vitro maturation fertilization and embryo development of mouse oocytes Cell J (Yakhteh) 2012; 13:259-264.

Golkar-Narenji A, Eimani H, Samadi F, Hasani S, Shahverdi AH, Eftekhari-Yazdi P, Kamalinejad M. Effect of Papaver rhoeas extract on in vitro maturation and developmental competence of immature mouse oocytes. Reprod Med Biol 2010; 9:211-215.

Author biographies is not available.