Relationship between MCP1 (-2518A>G) gene variants and ovarian cancer in Turkish population

R. Attar; S. G. Yilmaz; B. í‡akmakoğlu; S. Duman; G. Yildirim; U. Görmüş; T. ć°sbir

doi:10.14715/cmb/2017.63.8.21

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Vol. 63 No. 8: Issue 8

Issue Published : September 4, 2017

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Relationship between MCP1 (-2518A>G) gene variants and ovarian cancer in Turkish population

https://doi.org/10.14715/cmb/2017.63.8.21

R. Attar

Department of Obstetrics and Gynecology, Faculty of Medicine, Yeditepe University, Istanbul, Turkey

S. G. Yilmaz

Department of Molecular Medicine, Institute of Health Sciences, Yeditepe University, Istanbul, Turkey

B. í‡akmakoğlu

Department of Molecular Medicine, ASDETAE, Istanbul University, Istanbul, Turkey

S. Duman

Department of Molecular Medicine, Institute of Health Sciences, Yeditepe University, Istanbul, Turkey

G. Yildirim

Department of Obstetrics and Gynecology, Faculty of Medicine, Yeditepe University, Istanbul, Turkey

U. Görmüş

Department of Medical Genetics, Faculty of Medicine, Istinye University, Istinye University

T. ć°sbir

Department of Medical Biology, Faculty of Medicine, Yeditepe University, Istanbul, Turkey

Corresponding Author(s) : R. Attar

rattar@yeditepe.edu.tr

Cellular and Molecular Biology, Vol. 63 No. 8: Issue 8
Article Published : August 30, 2017

Abstract

The monocyte chemoattractant protein-1 (MCP-1) gene polymorphism(-2518A>G) in the regulatory region of the MCP-1 protein has been reported to be associated with cancer risk. In this study we aimed to investigate the relationship of MCP-1 (-2518A>G) gene polymorphism and ovarian cancer. MCP-1 genotyping was performed using polymerase chain reaction from blood samples ofovarian cancer patient (n=56) and a control groups (n=52).There was a significant difference in MCP1 (-2518A>G) genotypes between the patient and control groups (p=0.049; x2=6.042). AA carriers were significantly higher in the control group (p=0.014) whereas AG genotype and G allele carriers were significantly higher in the ovarian cancer group (p=0.029, p=0.014, respectively). This study suggests that MCP-1 (-2518A>G) AG genotype and G allele could be considered as risk factor for susceptibility to ovarian cancer.

Keywords

MCP1 (-2518A>G) Single nucleotide polymorphism Genetic variability Case-control study Ovarian cancer.

Attar, R., Yilmaz, S. G., í‡akmakoğlu, B., Duman, S., Yildirim, G., Görmüş, U., & ć°sbir, T. (2017). Relationship between MCP1 (-2518A>G) gene variants and ovarian cancer in Turkish population. Cellular and Molecular Biology, 63(8), 100–103. https://doi.org/10.14715/cmb/2017.63.8.21

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References

Siegal R, Naishadham D, Jemal A. Cancer statistics, 2012. CA Cancer J Clin 2012; 62: 10-29.
Conti I, Rollins BJ. CCL2 (monocyte chemoattractant protein-1) and cancer. Semin Cancer Biol 2004; 14(3): 149-154.
Wu J, Wei JJ. HMGA2 and high-grade serous ovarian carcinoma. J Mol Med (Berl) 2013; 91(10): 1155-1165.
Muralidhar GG, Barbolina MV. Chemokine receptors in epithelial ovarian cancer. Int J Mol Sci 2013; 15(1): 361-376.
Rovin BH, Lu L, Saxena R. A novel polymorphism in the MCP-1 gene regulatory region that influences MCP-1 expression. Biochem Biophys Res Commun 1999; 259(2): 344-348.
Luther SA, Cyster JG. Chemokines as regulators of T cell differentiation. Nat Immunol 2001; 2(2): 102-107.
Pham MH, Bonello GB, Castiblanco J, Le T, Sigala J, He W et al. The rs1024611 regulatory region polymorphism is associated with CCL2 allelic expression imbalance. PLoS One 2012; 7(11): e49498.
Szalai C, Duba J, Prohászka Z, Kalina í, Szabó T, Nagy B et al. Involvement of polymorphisms in the chemokine system in the susceptibility for coronary artery disease (CAD). Coincidence of elevated Lp(a) and MCP-1 -2518Gâ„G genotype in CAD patients. Atherosclerosis 2001; 158(1): 233–239.
Salcedo R, Ponce ML, Young HA, Wasserman K, Ward JM, Kleinman HK et al. Human endothelial cells express CCR2 and respond to MCP-1: direct role of MCP-1 in angiogenesis and tumor progression. Blood 2000; 96(1):34-40.
Kudo-Saito C, Shirako H, Ohike M, Tsukamoto N, Kawakami Y. CCL2 is critical for immunosuppression to promote cancer metastasis. Clin Exp Metastasis 2013; 30(4): 393-405.
Tanaka T, Terada M, Ariyoshi K, Morimoto K. Monocyte chemoattractant protein-1/CC chemokine ligand 2 enhances apoptotic cell removal by macrophages through Rac1 activation. Biochem Biophys Res Commun 2010; 399(4): 677-682.
Wei X, Tian Y, Lu W, Li W, Zhang M, Lu X et al. Functional polymorphisms in monocyte chemoattractant protein-1 are associated with increased susceptibility to ovarian cancer. DNA Cell Biol 2015; 34(1): 37-42.
Palmieri O, Latiano A, Salvatori E, Valvano MR, Bossa F, Latiano T et al. The -A2518G polymorphism of monocyte chemoattractant protein-1 is associated with Crohn's disease. Am J Gastroenterol 2010; 105(7): 1586-1594.
Pham MH, Bonello GB, Castiblanco J, Le T, Sigala J, He W et al. The rs1024611 regulatory region polymorphism is associated with CCL2 allelic expression imbalance. PLoS One 2012; 7(11): e49498.
Tse KP, Tsang NM, Chen KD, Li HP, Liang Y, Hsueh C et al. MCP-1 Promoter Polymorphism at 2518 is associated with metastasis of nasopharyngeal carcinoma after treatment. Clin Cancer Res 2007; 13(21): 6320-6326.
Pantsulaia I, Trofimov S, Kobyliansky E, Livshits G. Contribution of the familial and genetic factors on monocyte chemoattractant protein-1 variation in healthy human pedigrees. Cytokine 2005; 32(2): 117-123.

References

Siegal R, Naishadham D, Jemal A. Cancer statistics, 2012. CA Cancer J Clin 2012; 62: 10-29.

Conti I, Rollins BJ. CCL2 (monocyte chemoattractant protein-1) and cancer. Semin Cancer Biol 2004; 14(3): 149-154.

Wu J, Wei JJ. HMGA2 and high-grade serous ovarian carcinoma. J Mol Med (Berl) 2013; 91(10): 1155-1165.

Muralidhar GG, Barbolina MV. Chemokine receptors in epithelial ovarian cancer. Int J Mol Sci 2013; 15(1): 361-376.

Rovin BH, Lu L, Saxena R. A novel polymorphism in the MCP-1 gene regulatory region that influences MCP-1 expression. Biochem Biophys Res Commun 1999; 259(2): 344-348.

Luther SA, Cyster JG. Chemokines as regulators of T cell differentiation. Nat Immunol 2001; 2(2): 102-107.

Pham MH, Bonello GB, Castiblanco J, Le T, Sigala J, He W et al. The rs1024611 regulatory region polymorphism is associated with CCL2 allelic expression imbalance. PLoS One 2012; 7(11): e49498.

Szalai C, Duba J, Prohászka Z, Kalina í, Szabó T, Nagy B et al. Involvement of polymorphisms in the chemokine system in the susceptibility for coronary artery disease (CAD). Coincidence of elevated Lp(a) and MCP-1 -2518Gâ„G genotype in CAD patients. Atherosclerosis 2001; 158(1): 233–239.

Salcedo R, Ponce ML, Young HA, Wasserman K, Ward JM, Kleinman HK et al. Human endothelial cells express CCR2 and respond to MCP-1: direct role of MCP-1 in angiogenesis and tumor progression. Blood 2000; 96(1):34-40.

Kudo-Saito C, Shirako H, Ohike M, Tsukamoto N, Kawakami Y. CCL2 is critical for immunosuppression to promote cancer metastasis. Clin Exp Metastasis 2013; 30(4): 393-405.

Tanaka T, Terada M, Ariyoshi K, Morimoto K. Monocyte chemoattractant protein-1/CC chemokine ligand 2 enhances apoptotic cell removal by macrophages through Rac1 activation. Biochem Biophys Res Commun 2010; 399(4): 677-682.

Wei X, Tian Y, Lu W, Li W, Zhang M, Lu X et al. Functional polymorphisms in monocyte chemoattractant protein-1 are associated with increased susceptibility to ovarian cancer. DNA Cell Biol 2015; 34(1): 37-42.

Palmieri O, Latiano A, Salvatori E, Valvano MR, Bossa F, Latiano T et al. The -A2518G polymorphism of monocyte chemoattractant protein-1 is associated with Crohn's disease. Am J Gastroenterol 2010; 105(7): 1586-1594.

Pham MH, Bonello GB, Castiblanco J, Le T, Sigala J, He W et al. The rs1024611 regulatory region polymorphism is associated with CCL2 allelic expression imbalance. PLoS One 2012; 7(11): e49498.

Tse KP, Tsang NM, Chen KD, Li HP, Liang Y, Hsueh C et al. MCP-1 Promoter Polymorphism at 2518 is associated with metastasis of nasopharyngeal carcinoma after treatment. Clin Cancer Res 2007; 13(21): 6320-6326.

Pantsulaia I, Trofimov S, Kobyliansky E, Livshits G. Contribution of the familial and genetic factors on monocyte chemoattractant protein-1 variation in healthy human pedigrees. Cytokine 2005; 32(2): 117-123.

Author biographies is not available.