Comparison of two DNA extraction protocols from leave samples of Cotinus coggygria, Citrus sinensis and Genus juglans

F. Fallah; H. Minaei Chenar; H. Amiri; S. Omodipour; F. Shirbande Ghods; D. Kahrizi; M. Sohrabi; T. Ghorbani; E. Kazemi

doi:10.14715/cmb/2017.63.2.11

Issue

Vol. 63 No. 2: Issue 2

Issue Published : March 23, 2017

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

Comparison of two DNA extraction protocols from leave samples of Cotinus coggygria, Citrus sinensis and Genus juglans

https://doi.org/10.14715/cmb/2017.63.2.11

F. Fallah

Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran

H. Minaei Chenar

Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran

H. Amiri

Department of Forestry, Faculty of Agriculture, Razi University, Kermanshah, Iran

S. Omodipour

Department of Forestry, Faculty of Agriculture, Razi University, Kermanshah, Iran

F. Shirbande Ghods

Department of Forestry, Faculty of Agriculture, Razi University, Kermanshah, Iran

D. Kahrizi

Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran

M. Sohrabi

Zagros Bioidea Company, Razi University Incubator, Kermanshah, Iran

T. Ghorbani

Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran

E. Kazemi

Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Corresponding Author(s) : E. Kazemi

ekazemi2002@yahoo.com

Cellular and Molecular Biology, Vol. 63 No. 2: Issue 2
Article Published : February 28, 2017

Abstract

High quality DNA is essential for molecular research. Secondary metabolites can affect the quantity and quality DNA. In current research two DNA isolation methods including CTAB and Delaporta (protocols 1 & 2 respectively) were applied in three leave samples from Cotinus coggygria, Citrus sinensis and Genus juglans that their leaves are rich of secondary metabolites. We successfully isolated DNA from C. coggygria, C. sinensis and Genus Juglans using the two protocols described above. Good quality DNA was isolated from C. coggygria, C. sinensis and Genus Juglans using protocol 1, while protocol 2 failed to produce usable DNA from these sources. The highest amount of DNA (1.3-1.6) was obtained from them using protocol 1. As we discovered, procedure 1 may work better for plants with secondary metabolites.

Keywords

DNA extraction secondary metabolites Cotinus coggygria Citrus sinensis Genus juglans.

Fallah, F., Minaei Chenar, H., Amiri, H., Omodipour, S., Shirbande Ghods, F., Kahrizi, D., Sohrabi, M., Ghorbani, T., & Kazemi, E. (2017). Comparison of two DNA extraction protocols from leave samples of Cotinus coggygria, Citrus sinensis and Genus juglans. Cellular and Molecular Biology, 63(2), 76–78. https://doi.org/10.14715/cmb/2017.63.2.11