The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.
STAT1/2 is involved in the inhibition of cell growth induced by U0126 in Hela cells
Corresponding Author(s) : L Y Zhao
Cellular and Molecular Biology,
Vol. 55 No. 4: General Papers
Abstract
The mitogen-activated protein kinase (MAPK) signaling cascade plays an important role in cell life. Herein we show that small interfering RNAs targeting MAPK1 can inhibit HeLa cell growth and induce apoptosis along with up-regulation of signal transducers and activator 1 and 2 (STAT1/2). However, across-talk between the ras-raf-ERK1/2 signalling cascade and the JAK-STAT pathway remain largely unknown. Using MEK inhibitor U0126 and JAK-2 inhibitor AG490, we analyzed the relationship between ERK1/2 and STAT1/2 in HeLa cells. U0126 inhibited HeLa cell growth, arrested the cell cycle at G1/G0, and induced cell apoptosis, and AG490 partially reversed the effects of U0126. U0126 induced up-regulation of ERK1/2 and down-regulation of phosphorylated ERK1/2, increased STAT1 and STAT2 expression in a dose-dependent manner, and activated STAT1/2 via their phosphorylation. AG490 markedly inhibited the phosphorylation of STAT1 and STAT2 and slightly increased that of ERK1/2 inhibited by U0126. We suggest that STAT1/2 is involved in the inhibition of cell growth induced by U0126 in HeLa cells.
Keywords
U0126
AG490
ERK1/2
STAT1/2
HeLa cell
apoptosis.
Zhao, L. Y., Huang, C., Li, Z. F., Liu, L., Ni, L., & Song, T. S. (2009). STAT1/2 is involved in the inhibition of cell growth induced by U0126 in Hela cells. Cellular and Molecular Biology, 55(4), 1168–74. Retrieved from https://cellmolbiol.org/index.php/CMB/article/view/1059
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX