The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.
Effect of downregulated β-catenin on cell proliferative activity, the sensitivity to chemotherapy drug and tumorigenicity of ovarian cancer cells
Corresponding Author(s) : J. Dou
njdoujun@yahoo.com.cn
Cellular and Molecular Biology,
Vol. 57 No. 2: General Papers
Abstract
The role of Wnt/β-catenin signaling pathway in the etiology and/or progression of ovarian cancer has been well documented. It was domenstrated that ovarian cancer constantly exhibit constitutive activation of canonical Wnt signaling, usually as a result of oncogenic mutations that stabilize and dysregulate the β-catenin protein. In this study, we transfected an expression vector-based small hairpin RNA (shRNA) targeting to β-catenin encoding gene into human A2780 ovarian cancer cells to investigate the effects of β-catenin knockdown on biological characteristics of ovarian cancer cells. The results showed that β-catenin shRNA expression resulted in decreased β-catenin mRNA and protein expression in the transfected A2780 cells, inhibition of cellular proliferation, decreased capability of clonogenicity in the plating and the soft agar, and increased sensitivities to chemotherapy drugs vincristine, paclitaxel and cisplatin compared to untransfected cells. Importantly, we found that shRNA-mediated knockdown of β-catenin strongly decreases tumour growth of human A2780 ovarian cancer cells in xenografts. These results demonstrate that β-catenin might be an effective therapeutic target for human ovarian cancer treatment.
Keywords
Ovarian cancer cells
β-catenin
shRNA
tumorgenecity
target therapy.
Wang, J., Zhou, D., He, X., Wang, Y., Hu, W., Jiang, L., & Dou, J. (2011). Effect of downregulated β-catenin on cell proliferative activity, the sensitivity to chemotherapy drug and tumorigenicity of ovarian cancer cells. Cellular and Molecular Biology, 57(2), 1606–13. Retrieved from https://cellmolbiol.org/index.php/CMB/article/view/947
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX