Different ideas associated renal malformation and laminin Î±5 expression caused by maternal nicotine exposures
Corresponding Author(s) : M Shariati-Kohbanani
Cellular and Molecular Biology,
Vol. 62 No. 3: Issue 3
The number of smokers is increasing specially in pregnant mothers and millions of children with health problems are born from the smoker mothers. Nicotine as a toxic substance crosses from placenta and accumulates in the developing organs of fetus. In this study, the effects of maternal nicotine exposure on expression levels of kidney laminin Î±5 in newborn mice were examined. Timed pregnant mice were injected subcutaneously with nicotine at a dose of 2 mg/kg/day from day 7 of gestation to the last day of the pregnancy (Group 1) and from day 7 until the two weeks of postnatal (Group 2). Sham control groups were injected with saline. After the last injection, all the newborn mice were anesthetized; their kidneys were removed and prepared for analysis of mRNA and protein expression of laminin Î±5 using Real-Time PCR and immunohistochemical techniques, respectively. Our results showed that mRNA levels of kidney laminin Î±5 in newborn mice were increased in group 1 when compared to sham control group and also group 2. Immunohistochemical analysis demonstrated that the protein levels of laminin Î±5 in the glomerulus have significantly increased in group 1 when compared to group 2. In the proximal convoluted tubules, the parameter had a high significant increase in group 1 in comparison to control and also group 2. According to the results, it seems that maternal nicotine exposure may induce abnormal laminin Î±5 expression which may cause defects in kidney function during life time.
Laminin Î±5 proximal convoluted tubules glomerulus.
Shariati-Kohbanani, M., Taghavi, M. M., Shabanizadeh, A., Jafari Naveh, H. R., Taghipour, Z., & Kazemi Arababadi, M. (2016). Different ideas associated renal malformation and laminin Î±5 expression caused by maternal nicotine exposures. Cellular and Molecular Biology, 62(3), 100–104. Retrieved from https://cellmolbiol.org/index.php/CMB/article/view/827
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