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Copyright (c) 2025 Aarón Ernesto Marure-Rojano , José Ricardo Cano-García , Alexandra Berenice Luna-Agulo , Laura Sánchez-Chapul , Clara Leticia Santos-Cuevas , María del Rocío Aguilar-Gaytán , Ericka Patricia Flores-Berrios, Beatriz del Carmen Couder-Garcia , Gabriel Lara-Hernández, Ivan Uriel Bahena-Ocampo , Carlos Landa-Solís
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.The cytotoxic effect of quercetin-induced apoptosis on lung metastatic cells from giant cell tumor of bone
Corresponding Author(s) : Carlos Landa-Solís
Cellular and Molecular Biology,
Vol. 71 No. 5: Issue 5
Abstract
The pulmonary parenchyma is the primary site of metastasis for giant cell tumor (GCT) of bone, a benign yet aggressive musculoskeletal tumor. Current treatments, including surgery and antibody therapy, are only partially effective and often lead to significant side effects. This study aimed to evaluate the apoptotic activity of quercetin, a naturally occurring flavonoid with anticancer properties, on metastatic GCT lung cells (TIB-223). The immunophenotype of the TIB-223 cell line was characterized using flow cytometry, revealing positivity for CD166 and CD47 markers and negativity for CD34, CD73, CD117, CD45, and fibroblast markers. The IC50 of quercetin was determined at 91.1 µM through MTT assays, demonstrating its cytotoxic effect in a dose-dependent manner. Apoptosis was confirmed via flow cytometry and Western blotting, showing increased caspase-3 expression after 24 hours of treatment. These findings indicate that quercetin induces apoptosis in metastatic GCT cells and could serve as a basis for developing phytopharmaceutical therapies targeting this pathology.
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