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Copyright (c) 2024 Han Wang, Xiang Li, Yundong Xu, Yaping Tian, Qidan Li, Yongzhen Zhang, Xu Wang, Juan Ni
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.EGCG inhibits the oxidative damage induced by TiO2-NPs in human colon cell lines
Corresponding Author(s) : Juan Ni
Cellular and Molecular Biology,
Vol. 70 No. 11: Issue 11
Abstract
To assess the protective effects of (-)-Epigallocatechin-3-gallate (EGCG), a natural antioxidant, against cellular oxidative damage induced by titanium dioxide nanoparticles (TiO2-NPs), Human Colon cells NCM460 and Colon Cancer cells SW620 were selected for this study. The cells were divided into three groups: control group, TiO2-NPs (80 μg/mL) exposure group, and EGCG (20 μmol/L)+TiO2-NPs (80 μg/mL) co-exposure group. The study evaluated the precipitation rate of TiO2-NPs influenced by EGCG in a cell-free system. It also measured the levels of ROS, MDA, and total antioxidant capacity in the cells of each group. The uptake of TiO2-NPs by the cells was assessed using the SSCe/SSC0 ratio, and genome instability was evaluated. The results demonstrated that the addition of 20 μmol/L EGCG to the system resulted in greater sedimentation of TiO2-NPs compared to TiO2-NPs alone (P<0.05). The SSCe/SSC0 values in the co-exposure group were significantly lower than those in the TiO2-NPs alone group (P<0.001). TiO2-NPs induced a higher oxidative stress index in the cells (P<0.001), while the co-exposure group exhibited a lower REDOX index (P<0.001). The combination of EGCG and TiO2-NPs did not significantly affect genome instability in either cell line. Importantly, EGCG showed a certain inhibitory effect on oxidative damage to colon cells induced by TiO2-NPs, with no significant difference observed between normal and cancer cells in terms of this protective effect. Conducting a comprehensive investigation into the interaction mechanism between EGCG and TiO2-NPs is crucial for establishing a scientific foundation that can guide the optimal utilization of the antioxidant properties of EGCG to mitigate the toxicity associated with TiO2-NPs.
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