Issue
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.
Mesenchymal Stem Cell physiology can be affected by antibiotics: An in vitro study
Corresponding Author(s) : I. Pountos
pountos@doctors.org.uk
Cellular and Molecular Biology,
Vol. 60 No. 4: Issues 4
Abstract
The aim of this study was to investigate the effect of antibiotics used in clinical practice on Mesenchymal Stem Cells (MSCs) potential to proliferate and differentiate towards an osteogenic lineage. Trabecular bone was obtained from 10 patients (mean age of 36 years, range 18-72) suffering from long bone fractures. Mesenchymal Stem Cells (MSCs) were isolated and functional assays on their proliferation (CFU-F and XTT) and osteogenic differentiation (alkaline phosphatase activity and total calcium production) were performed. The effect of medium supplementation with gentamicin, vancomycin, benzyl-penicillin, flucloxacillin, cefuroxime and metronidazole was analysed. In concentrations found in peripheral circulation, none of the studies antibiotics had an effect on MSCs ability to proliferate and differentiate towards osteogenic lineage. Vancomycin and gentamicin in concentrations of 200 μg/ml and 75 μg/ml respectively, down-regulated the proliferation and osteogenic activity of MSCs. Some combination of the studied antibiotics found to inhibit both proliferation and osteogenesis. High antibiotic concentrations and the combination of different formulations can have detrimental effects on osteoprogenitor cells physiology and potentially bone healing.
Keywords
Mesenchymal Stem Cells
antibiotics
Bone healing
Non-union.
Pountos, I., Georgouli, T., Henshaw, K., Howard, B., & Giannoudis, P. V. (2014). Mesenchymal Stem Cell physiology can be affected by antibiotics: An in vitro study. Cellular and Molecular Biology, 60(4), 1–7. Retrieved from https://cellmolbiol.org/index.php/CMB/article/view/512
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX