Jun  Chen; Kaixin Zhang; Yi  Yang; Jianqiang Wu

doi:10.14715/cmb/2023.69.9.32

Issue

Vol. 69 No. 9: Issue 9

Issue Published : October 8, 2023

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MiR-204-5p regulates HUVEC cell inflammation and apoptosis by targeting P4HB

miR-204-5p regulates HUVEC inflammation

https://doi.org/10.14715/cmb/2023.69.9.32

Jun Chen

Department of Vascular Surgery, Suqian Hospital Affiliated to Xuzhou Medical University, (Nanjing Drum Tower Hospital Group Suqian Hospital), Suqian, 223800, China

Kaixin Zhang

Department of Vascular Surgery, Suqian Hospital Affiliated to Xuzhou Medical University, (Nanjing Drum Tower Hospital Group Suqian Hospital), Suqian, 223800, China

Yi Yang

Department of Vascular Surgery, Suqian Hospital Affiliated to Xuzhou Medical University, (Nanjing Drum Tower Hospital Group Suqian Hospital), Suqian, 223800, China

Jianqiang Wu

Department of General Surgery, Suqian Hospital Affiliated to Xuzhou Medical University, (Nanjing Drum Tower Hospital Group Suqian Hospital), Suqian, 223800, China

Corresponding Author(s) : Jianqiang Wu

jianqian_wu@publisher.org.cn

Cellular and Molecular Biology, Vol. 69 No. 9: Issue 9
Article Published : September 30, 2023

Abstract

The purpose of this study arose to investigate the mechanism of miR-204-5p targeting P4HB to regulate inflammation and apoptosis in HUVEC cells. Serum specimens were obtained from lower extremity DVT patients and healthy subjects. Targetscan predicted P4HB as a target gene for miR-204-5p. A dual luciferase reporter assay was conducted to determine the modulating effect of miR-204-5p on P4HB. qRT-PCR was used to detect miR-204-5p and P4HB expression. Established CoCl2-induced hypoxia/ischemia model of HUVEC, transfected with miR-204-5p mimics and pcDNA3. 1-P4HB. CCK-8 assay for cell viability. Apoptosis was assayed by flow cytometry, western blot and western blot. Immunofluorescence and ELISA were carried out to detect ROS, MDA, SOD, LDH, GSH-px, TNF-α, IL-1β and IL-6 expression. miR-204-5p was reduced markedly in the sera of DVT patients. miR-204-5p negatively regulated P4HB. P4HB expression was raised in the sera of DVT patients. Exposure to CoCl2 decreased miR-204-5p expression and increased P4HB in HUVEC. Over-expressed miR-204-5p effectively increased cell viability and inhibited apoptosis; its effect was counteracted by continued overexpression of P4HB. In addition, miR- 204-5p mimics clearly reduced CoCl2-induced ROS and inflammation, and pcDNA3. 1-P4HB acted counteractively. miR-204-5p may inhibit HUVEC proliferation, ROS generation and cellular inflammation through negative regulation of P4HB. miR-204-5p promises to become a potential target for DVT therapy.

Keywords

miR-204-5p P4HB inflammation apoptosis deep vein thrombosis HUVEC cell

Chen, J. ., Zhang, K., Yang, Y. ., & Wu, J. (2023). MiR-204-5p regulates HUVEC cell inflammation and apoptosis by targeting P4HB: miR-204-5p regulates HUVEC inflammation. Cellular and Molecular Biology, 69(9), 207–212. https://doi.org/10.14715/cmb/2023.69.9.32