Jing  Zhang; Yingyu  Quan; Xiaoxia  Su; Baowei  Qiu; Qi Dong

doi:10.14715/cmb/2023.69.8.36

Issue

Vol. 69 No. 8: Issue 8

Issue Published : September 15, 2023

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Circ-PRMT5 stimulates the proliferative ability in Wilms’ tumor through the miR-7-5p/KLF4 axis

Circ-PRMT5 stimulates the development of Wilms’ tumor

https://doi.org/10.14715/cmb/2023.69.8.36

Jing Zhang

Department of General Surgery, Hainan Women and Children’s Medical Center, Haikou, China

Yingyu Quan

Department of Pediatrics, Hainan General Hospital Hainan Affiliated Hospital of Hainan Medical University, Haikou, China

Xiaoxia Su

Department of General Surgery, Hainan Women and Children’s Medical Center, Haikou, China

Baowei Qiu

Department of General Surgery, Hainan Women and Children’s Medical Center, Haikou, China

Qi Dong

Department of General Surgery, Hainan Women and Children’s Medical Center, Haikou, China

Corresponding Author(s) : Qi Dong

qi_dong@publisher.org.cn

Cellular and Molecular Biology, Vol. 69 No. 8: Issue 8
Article Published : August 31, 2023

Abstract

CircRNAs are extensively discovered in mammals and they are closely linked to tumor cell behaviors. This study aims to detect the expression pattern of circ-PRMT5 in Wilms’ tumor and its ability in influencing tumor development. Circ-PRMT5 levels in Wilms’ tumor samples were detected. The regulatory effect of circ-PRMT5 on proliferative ability in Wilms’ tumor cells was assessed by cell counting kit-8 (CCK-8), colony formation and 5-Ethynyl-2’- deoxyuridine (EdU) assay. The interaction in the circ-PRMT5/miR-7-5p/KLF4 axis was determined by luciferase assay. Rescue experiments were conducted to reveal the role of the circ-PRMT5/miR-7-5p/KLF4 axis in Wilms’ tumor development. Circ-PRMT5 was highly expressed in Wilms’ tumor samples. High levels of circ-PRMT5 predicted advanced tumor staging in patients with Wilms’ tumor. Knockdown of circ-PRMT5 markedly suppressed proliferative ability in Wilms’ tumor cells. Luciferase assay confirmed the interaction in the circ-PRMT5/miR-7-5p/KLF4 axis. Rescue experiments finally identified that circ-PRMT5 stimulated the malignant development of Wilms’ tumor by activating the miR-7-5p/KLF4 axis. Circ-PRMT5 is upregulated in Wilms’ tumor samples, which is closely linked to its tumor staging. It stimulates proliferative ability in Wilms’ tumor cells by activating the miR-7-5p/KLF4 axis.

Keywords

Circ-PRMT5 MiR-7-5p KLF4 Wilms’ tumor Proliferation

Zhang, J. ., Quan, Y. ., Su, X. ., Qiu, B. ., & Dong, Q. (2023). Circ-PRMT5 stimulates the proliferative ability in Wilms’ tumor through the miR-7-5p/KLF4 axis: Circ-PRMT5 stimulates the development of Wilms’ tumor. Cellular and Molecular Biology, 69(8), 232–236. https://doi.org/10.14715/cmb/2023.69.8.36