Faik Sertel Secer; Ilker  Yavas; Zafer  Cantekin; Yusuf  Bozkurt; Tugba  Korkmaz Yavas; Alexander  Atanasoff

doi:10.14715/cmb/2023.69.8.22

Issue

Vol. 69 No. 8: Issue 8

Issue Published : September 15, 2023

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Effect of Different Plant Extracts on Microbial Population in The Frozen African Catfish (Clarias gariepinus) Semen

Plant Extracts' Impact on Microbial Count in Frozen Catfish Semen

https://doi.org/10.14715/cmb/2023.69.8.22

Faik Sertel Secer

Ankara University, Faculty of Agriculture, 06110 Ankara, Turkiye

Ilker Yavas

Mustafa Kemal University, Faculty of Veterinary Medicine, Hatay, Turkiye

Zafer Cantekin

Mustafa Kemal University, Faculty of Veterinary Medicine, Hatay, Turkiye

Yusuf Bozkurt

İskenderun Technical University, Faculty of Marine Sciences and Technology, Hatay, Turkiye

Tugba Korkmaz Yavas

Republic of Turkiye, Ministry of Agriculture and Forestry Hatay, Turkiye

Alexander Atanasoff

Trakia University, Faculty of Veterinary Medicine, Stara Zagora, Bulgaria

Corresponding Author(s) : Faik Sertel Secer

sertelsecer@yahoo.com

Cellular and Molecular Biology, Vol. 69 No. 8: Issue 8
Article Published : August 31, 2023

Abstract

Fish sperm cryopreservation has been attempted on roughly freshwater and marine species since 1953. This study sought to assess the potential of various plant extracts to function as natural antimicrobial agents in the frozen semen of African catfish (Clarias gariepinus). Diluted sperm was packaged in 0.25ml straws and left for 10min equilibration at 4°C. Following equilibration, the straws were exposed to liquid nitrogen vapor for 10 min and plunged into the liquid nitrogen (-196°C) and then thawed in a water bath at 35°C for 20s. Sperm samples were put into sterile 1.5 ml tubes immediately after thawing and the microbial count was detected with classical microbiological culture method. In the results of microbiological analyses, these tree plant extracts especially Echinacea purpurea were found highly effective for decreasing bacterial contamination levels of African catfish (C. gariepinus) semen. These plant extracts may have the potential for antibacterial effect, and they can be useful for the dilution of semen.

Keywords

African catfish Bacterial count Clarias gariepinus Plant extract Semen

Secer, F. S., Yavas, I. ., Cantekin, Z. ., Bozkurt, Y. ., Korkmaz Yavas, T. ., & Atanasoff, A. . (2023). Effect of Different Plant Extracts on Microbial Population in The Frozen African Catfish (Clarias gariepinus) Semen: Plant Extracts’ Impact on Microbial Count in Frozen Catfish Semen. Cellular and Molecular Biology, 69(8), 144–147. https://doi.org/10.14715/cmb/2023.69.8.22