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Regulation effect of miR-7 on intervening colorectal cancer rats with HP infection through Akt/GSK-3β/β-catenin pathway
Corresponding Author(s) : Jiaxi Chen
Cellular and Molecular Biology,
Vol. 68 No. 6: Issue 6
Abstract
This experiment aimed to analysis of the intervention effects of modulating miR-7 on rats with colorectal cancer complicated with HP infection and the effects on (serine/threonine kinase) Akt / (glycogen synthase kinase 3 β) GSK-3 β/ ( β- β - Catenin) β- Influence of the catenin pathway. For this purpose, forty special pathogen-free (SPF) - grade rats of both sexes were randomly divided into 10 control, 10 colorectal cancer and HP infection model groups, 10 up-regulated miR-7, and 10 down-regulated miR-7 groups. Observational analysis of rat colon tissues was performed using the HE staining method. Detection of inflammatory factors [TNF- α、 IL-8, IL-6], detection of miR-7 expression, detection of Akt, GSK-3 using Western blot β、β- Catenin protein expression. Results showed that forty special pathogen-free (SPF) - grade rats of both sexes were randomly divided into 10 control, 10 colorectal cancer and HP infection model groups, 10 up-regulated miR-7, and 10 down-regulated miR-7 groups. Observational analysis of rat colon tissues was performed using the HE staining method. Detection of inflammatory factors [TNF- α、 IL-8, IL-6], detection of miR-7 expression, detection of Akt, GSK-3 using Western blot β、β- Catenin protein expression. It was concluded that modulation of miR-7 in rats with colorectal cancer and HP infection enables regulation of the Akt / GSK-3 β/β- Catenin pathway to improve serum inflammation condition and alleviate HP infection in rats, which played a better role in intervention.
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