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Copyright (c) 2022 Hasanain Riyadh Al- Isawi, Faiza Safi, Hayder A. Muhammed
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The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.Association of CTLA-4 (+49A/G) gene Polymorphism with type 1 diabetes mellitus in Iraqi children
Corresponding Author(s) : Hasanain Riyadh Al- Isawi
Cellular and Molecular Biology,
Vol. 68 No. 5: Issue 5
Abstract
CTLA4 is a regulator gene for T cells and relates to autoimmune diseases. By using a case-control method, CTLA4 functional single-nucleotide polymorphisms for potential associations with Type 1 diabetes mellitus in an Iraqi children's population. ARMS-PCR method is used for genotyping +49AG (rs231775) variations in 60 obese children and 60 ethnically matched controls; all measured subjects were (fasting glucose, fasting insulin, and HbA1c). The glucose oxidase method is used to determine plasma glucose levels. The amounts of insulin in the blood were determined using a radioimmunoassay (RIA); Insulin resistance was measured using the HOMA-IR index. A HOMA-IR cut-off level of 2.5 was acceptable. There was no significant difference in allele and genotype frequencies between the groups, according to CTLA4 +49AG analyses. In conclusion, AA cases had a high frequency of A/A genotype than healthy participants but lower rates of A/G and G/G genotypes.
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