Effect of Cerium Oxide Nanoparticles on Myocardial Cell Apoptosis Induced by Myocardial Ischemia-Reperfusion Injury

Effect of Cerium Oxide Nanoparticles on Myocardial Cell Apoptosis

Abstract

This study was to provide a theoretical basis for effective treatment of myocardial ischemia-reperfusion injury (I/R injury) and explore the effect of cerium oxide (CeO₂) nanoparticles on myocardial cell apoptosis induced by I/R injury. In this study, 50 healthy male Sprague Dawley (SD) rats were selected and divided into five groups according to the random table method: a sham operation group, an I/R group, a 1 - 10 nm CeO₂ nanoparticle group (CeO₂-1 group), a 10 - 25 nm CeO₂ nanoparticle group (CeO₂-2 group), and a 50 nm CeO₂ nanoparticle group (CeO₂-3 group). Rats in different groups were injected with phosphate buffer solution (PBS) and CeO₂ nanoparticles with different diameters, respectively. The rat models of I/R injury were prepared to explore and analyze the superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, glutathione peroxidase (GSH-Px) activity, and myocardial cell apoptosis of rats with I/R injury by CeO₂ nanoparticles. The results showed that the cardiomyocyte necrosis, SOD activity, MDA content, GSH-Px activity, and apoptosis index of the three groups of rats injected with CeO₂ nanoparticles were much better than those in the I/R group. The effects on SOD activity, MDA content, GSH-Px activity, and apoptosis index of cardiomyocytes in the CeO₂-2 group were significantly better than those in the CeO₂-1 and CeO₂-3 groups, showing statistically great differences (P< 0.05); and effects on SOD activity, MDA content, and GSH-Px activity of cardiomyocytes in CeO₂-1 group were better obviously than those in the CeO₂-3 groups, showing statistically observable differences (P< 0.05). In addition, the difference between the CeO₂-1 group and CeO₂-3 on the apoptosis index of cardiomyocytes was not statistically remarkable (P> 0.05). It can be considered that the CeO₂ nanoparticles can effectively alleviate the effects of myocardial I/R injury, showing reliable clinical significance.