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Vol. 66 No. 5: Issue 5

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Resveratrol inhibits skin squamous cell carcinoma proliferation, migration and invasion through up-regulating miR-126

https://doi.org/10.14715/cmb/2020.66.5.25
Bo Zhang
Cosmetic Laser Center, Plastic Surgery Hospital of Chinese Academic of Medical Science, Peking Union Medical College, Beijing, 100144,China
Moslem Lari Najafi
Pharmaceutical Sciences and Cosmetic Products Research Center, Kerman University of Medical Sciences, Kerman, Iran

Corresponding Author(s) : Bo Zhang

sanpi373737fb@126.com

Cellular and Molecular Biology, Vol. 66 No. 5: Issue 5

Abstract

The current experiment was performed to explore the effect and possible mechanism of resveratrol on the proliferation, migration and invasion of skin squamous cell carcinoma cells (HSC-5). Tetramethyl azozo blue (MTT) method, Transwell experiment and real-time fluorescence quantitative PCR (RT-qPCR) were used to detect the effects of resveratrol intervention on the proliferation, migration and invasion of HSC-5 cells and the expression of miR-126. The miR-126 mimics and inhibitors were transfected into HSC-5 cells, and the effects of up-regulation or down-regulation of HSC-5 expression on the proliferation, migration and invasion of HSC-5 cells were detected. The miR-126 inhibitor was transfected into HSC-5 cells, and the effects of resveratrol intervention on HSC-5 cell proliferation, migration and invasion, and β-catenin protein expression were detected. After resveratrol intervention, the growth rate, migration and invasion of HSC-5 cells were significantly reduced, miR-126 expression was significantly increased, and β-catenin protein expression was significantly reduced (p<0.05). After up-regulating the expression of miR-126, the growth rate, migration and invasion of HSC-5 cells were significantly reduced (p<0.05). After down-regulating the expression of miR-126, the growth rate, migration and invasion of HSC-5 cells were significantly increased (p<0.05). Down-regulation of miR-126 expression could reverse the effects of resveratrol intervention on HSC-5 cell proliferation, migration and invasion, and β-catenin protein expression (p<0.05). Resveratrol could inhibit the proliferation, migration and invasion of skin squamous cell carcinoma cells, which may be related to the up-regulation of miR-126 to inhibit the Wnt / β-catenin signaling pathway.

Keywords

Resveratrol skin squamous cell carcinoma miR-126 cell proliferation migration and invasion Wnt / β-catenin signaling pathway
Zhang, B., & Lari Najafi, M. (2020). Resveratrol inhibits skin squamous cell carcinoma proliferation, migration and invasion through up-regulating miR-126. Cellular and Molecular Biology, 66(5), 142–147. https://doi.org/10.14715/cmb/2020.66.5.25
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