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Vol. 65 No. 7: Issue 7

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Clinical significance of cell-free DNA concentration and integrity in serum of gastric cancer patients before and after surgery

https://doi.org/10.14715/cmb/2019.65.7.19
Xueliang Zhang
Department of General Surgery, Nantong Tumor Hospital. No.48, West Youth Road, 226361 Nantong, Jiangsu province, China
Zhenfeng Wu
Department of Surgical Oncology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine. No.155 Hanzhong Road, Qinhuai District, Nanjing 210029, Jiangsu province, China
Qian Shen
Department of Oncology, Nantong Tumor Hospital. No.48, West Youth Road, 226000 Nantong, Jiangsu province, China
Rong Li
Department of Medical oncology, Taikang Xianlin Drum Tower Hospital. No.188 Lingshan North Road, Qixia District, Nanjing 210000, Jiangsu province, China
Xiaohui Jiang
Department of General Surgery, Nantong Tumor Hospital. No.48, West Youth Road, 226361 Nantong, Jiangsu province, China
Jindong Wu
Department of General Surgery, Nantong Tumor Hospital. No.48, West Youth Road, 226361 Nantong, Jiangsu province, China
Ding Li
Department of General Surgery, Nantong Tumor Hospital. No.48, West Youth Road, 226361 Nantong, Jiangsu province, China
Ding Wang
Department of General Surgery, Nantong Tumor Hospital. No.48, West Youth Road, 226361 Nantong, Jiangsu province, China
Chang Zou
Clinical Medical Research Center, The Second Clinical Medical School of Jinan University, Shenzhen People's Hospital. Clinical Medical Research Center, The Second Clinical College of Jinan University, Shenzhen People's Hospital, Room 302, 8th Building, No. 1017, Dongmen North Road, Shenzhen 518020, China
Yuejiao Zhong
Department of Medical Oncology, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & The Affiliated Cancer Hospital of Nanjing Medical University. No.42, Baiziting, Xuanwu District, Nanjing 210009, Jiangsu Province, China
Xianfeng Cheng
Clinic laboratory of Institute of Dermatology and Hospital for Skin Diseases, Chinese Academy of Medical Sciences. No.12, Jiangwangmiao Street, Xuanwu District, Nanjing 210042, Jiangsu Province, China

Corresponding Author(s) : Xueliang Zhang

huihd4@163.com

Cellular and Molecular Biology, Vol. 65 No. 7: Issue 7

Abstract

Early onset of gastric cancer (GC) is almost asymptomatic, thereby making early diagnosis and early treatment difficult. Blood samples were taken from 90 GC patients who had not undergone surgery, and from another set of 110 GC patients who had undergone surgery. The control consisted of 90 healthy individuals. Cell-free DNA (cfDNA) and its integrity were assayed using qPCR. The association between cfDNA levels and clinical presentations of GC was analyzed. In addition, cfDNA, carcino-embryonic antigen (CEA), carbohydrate antigen 724 (CA724), carbohydrate antigen 125 (CA125) and carbohydrate antigen 199 (CA199) were subjected to specificity and sensitivity analyses using ROC. The levels of cfDNA of GC patients before surgery were markedly higher than corresponding values in patients with GC after surgery. Post-surgery, the two indices were also significantly higher in GC patients than in the healthy group. The correlation between cfDNA concentration/integrity and gender, age, TNM stage, tumor differentiation, tumor location, neuronspecific enolase (NSE), or alpha fetoprotein (AFP) expression, was not significant in GC patients before or after surgery. However, the correlation between cfDNA and concentrations of CEA/CA125 was significant. The CA199 expression level was significantly correlated with cfDNA integrity. The AUC values of cfDNA concentration and integrity were higher than other tumor markers. Measurement of cfDNA concentration and integrity may be an ideal tumor screening method with higher sensitivity and specificity than traditional tumor biomarkers. The cfDNA concentration and integrity are significantly increased in plasma of GC patients, and may serve as promising indicators for GC.

Keywords

Gastric cancer Cell-free DNA Quantitative PCR ROC curve.
Zhang, X., Wu, Z., Shen, Q., Li, R., Jiang, X., Wu, J., Li, D., Wang, D., Zou, C., Zhong, Y., & Cheng, X. (2019). Clinical significance of cell-free DNA concentration and integrity in serum of gastric cancer patients before and after surgery. Cellular and Molecular Biology, 65(7), 111–117. https://doi.org/10.14715/cmb/2019.65.7.19
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