Issue

Vol. 65 No. 7: Issue 7

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

Clinical significance of cell-free DNA concentration and integrity in serum of gastric cancer patients before and after surgery

https://doi.org/10.14715/cmb/2019.65.7.19
Xueliang Zhang
Department of General Surgery, Nantong Tumor Hospital. No.48, West Youth Road, 226361 Nantong, Jiangsu province, China
Zhenfeng Wu
Department of Surgical Oncology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine. No.155 Hanzhong Road, Qinhuai District, Nanjing 210029, Jiangsu province, China
Qian Shen
Department of Oncology, Nantong Tumor Hospital. No.48, West Youth Road, 226000 Nantong, Jiangsu province, China
Rong Li
Department of Medical oncology, Taikang Xianlin Drum Tower Hospital. No.188 Lingshan North Road, Qixia District, Nanjing 210000, Jiangsu province, China
Xiaohui Jiang
Department of General Surgery, Nantong Tumor Hospital. No.48, West Youth Road, 226361 Nantong, Jiangsu province, China
Jindong Wu
Department of General Surgery, Nantong Tumor Hospital. No.48, West Youth Road, 226361 Nantong, Jiangsu province, China
Ding Li
Department of General Surgery, Nantong Tumor Hospital. No.48, West Youth Road, 226361 Nantong, Jiangsu province, China
Ding Wang
Department of General Surgery, Nantong Tumor Hospital. No.48, West Youth Road, 226361 Nantong, Jiangsu province, China
Chang Zou
Clinical Medical Research Center, The Second Clinical Medical School of Jinan University, Shenzhen People's Hospital. Clinical Medical Research Center, The Second Clinical College of Jinan University, Shenzhen People's Hospital, Room 302, 8th Building, No. 1017, Dongmen North Road, Shenzhen 518020, China
Yuejiao Zhong
Department of Medical Oncology, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & The Affiliated Cancer Hospital of Nanjing Medical University. No.42, Baiziting, Xuanwu District, Nanjing 210009, Jiangsu Province, China
Xianfeng Cheng
Clinic laboratory of Institute of Dermatology and Hospital for Skin Diseases, Chinese Academy of Medical Sciences. No.12, Jiangwangmiao Street, Xuanwu District, Nanjing 210042, Jiangsu Province, China

Corresponding Author(s) : Xueliang Zhang

huihd4@163.com

Cellular and Molecular Biology, Vol. 65 No. 7: Issue 7

Abstract

Early onset of gastric cancer (GC) is almost asymptomatic, thereby making early diagnosis and early treatment difficult. Blood samples were taken from 90 GC patients who had not undergone surgery, and from another set of 110 GC patients who had undergone surgery. The control consisted of 90 healthy individuals. Cell-free DNA (cfDNA) and its integrity were assayed using qPCR. The association between cfDNA levels and clinical presentations of GC was analyzed. In addition, cfDNA, carcino-embryonic antigen (CEA), carbohydrate antigen 724 (CA724), carbohydrate antigen 125 (CA125) and carbohydrate antigen 199 (CA199) were subjected to specificity and sensitivity analyses using ROC. The levels of cfDNA of GC patients before surgery were markedly higher than corresponding values in patients with GC after surgery. Post-surgery, the two indices were also significantly higher in GC patients than in the healthy group. The correlation between cfDNA concentration/integrity and gender, age, TNM stage, tumor differentiation, tumor location, neuronspecific enolase (NSE), or alpha fetoprotein (AFP) expression, was not significant in GC patients before or after surgery. However, the correlation between cfDNA and concentrations of CEA/CA125 was significant. The CA199 expression level was significantly correlated with cfDNA integrity. The AUC values of cfDNA concentration and integrity were higher than other tumor markers. Measurement of cfDNA concentration and integrity may be an ideal tumor screening method with higher sensitivity and specificity than traditional tumor biomarkers. The cfDNA concentration and integrity are significantly increased in plasma of GC patients, and may serve as promising indicators for GC.

Keywords

Gastric cancer Cell-free DNA Quantitative PCR ROC curve.
Zhang, X., Wu, Z., Shen, Q., Li, R., Jiang, X., Wu, J., Li, D., Wang, D., Zou, C., Zhong, Y., & Cheng, X. (2019). Clinical significance of cell-free DNA concentration and integrity in serum of gastric cancer patients before and after surgery. Cellular and Molecular Biology, 65(7), 111–117. https://doi.org/10.14715/cmb/2019.65.7.19
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. Figueiredo C, Camargo MC, Leite M, Fuentes-Panana EM, Rabkin CS, Machado JC. Pathogenesis of Gastric Cancer: Genetics and Molecular Classification. Curr Top Microbiol Immunol 2017; 400: 277-304.
  2. Hamashima C. Current issues and future perspectives of gastric cancer screening. World J Gastroenterol 2014; 20: 13767-13774.
  3. Zhu Z. [2017 hotspots review and outlook on gastric cancer surgery in China]. Zhonghua Wei Chang Wai Ke Za Zhi 2018; 21: 7-14.
  4. Zhang XY, Zhang PY. Gastric cancer: somatic genetics as a guide to therapy. J Med Genet 2017; 54: 305-312.
  5. Bornschein J, Leja M, Kupcinskas J, Link A, Weaver J, Rugge M, et al. Molecular diagnostics in gastric cancer. Front Biosci (Landmark Ed) 2014; 19: 312-338.
  6. Asaka M, Mabe K. Strategies for eliminating death from gastric cancer in Japan. Proc Jpn Acad Ser B Phys Biol Sci 2014; 90: 251-258.
  7. de Angelis GL, Cavallaro LG, Maffini V, Moussa AM, Fornaroli F, Liatopoulou S, et al. Usefulness of a serological panel test in the assessment of gastritis in symptomatic children. Dig Dis 2007; 25: 206-213.
  8. Feng F, Tian Y, Xu G, Liu Z, Liu S, Zheng G, et al. Diagnostic and prognostic value of CEA, CA19-9, AFP and CA125 for early gastric cancer. BMC Cancer 2017; 17: 737.
  9. Yu J, Zheng W. An Alternative Method for Screening Gastric Cancer Based on Serum Levels of CEA, CA19-9, and CA72-4. J Gastrointest Cancer 2018; 49: 57-62.
  10. Liang Y, Wang W, Fang C, Raj SS, Hu WM, Li QW, et al. Clinical significance and diagnostic value of serum CEA, CA19-9 and CA72-4 in patients with gastric cancer. Oncotarget 2016; 7: 49565-49573.
  11. Muinelo-Romay L, Casas-Arozamena C, Abal M. Liquid Biopsy in Endometrial Cancer: New Opportunities for Personalized Oncology. Int J Mol Sci 2018; 19: 8.
  12. Normanno N, Cervantes A, Ciardiello F, De Luca A, Pinto C. The liquid biopsy in the management of colorectal cancer patients: Current applications and future scenarios. Cancer Treat Rev 2018; 70: 1-8.
  13. Malan V, Bussieres L, Winer N, Jais JP, Baptiste A, Le Lorc'h M, et al. Effect of Cell-Free DNA Screening vs Direct Invasive Diagnosis on Miscarriage Rates in Women With Pregnancies at High Risk of Trisomy 21: A Randomized Clinical Trial. JAMA 2018; 320: 557-565.
  14. Diehl F, Schmidt K, Choti MA, Romans K, Goodman S, Li M, et al. Circulating mutant DNA to assess tumor dynamics. Nat Med 2008; 14: 985-990.
  15. Leon SA, Shapiro B, Sklaroff DM, Yaros MJ. Free DNA in the serum of cancer patients and the effect of therapy. Cancer Res 1977; 37: 646-650.
  16. Stroun M, Anker P. Circulating DNA in higher organisms cancer detection brings back to life an ignored phenomenon. Cell Mol Biol (Noisy-le-grand) 2005; 51: 767-774.
  17. Laufer-Geva S, Rozenblum AB, Twito T, Grinberg R, Dvir A, Soussan-Gutman L, et al. The Clinical Impact of Comprehensive Genomic Testing of Circulating Cell-Free DNA in Advanced Lung Cancer. J Thorac Oncol 2018; 13: 1705-1716.
  18. Baraniskin A, Kuhnhenn J, Schlegel U, Schmiegel W, Hahn S, Schroers R. MicroRNAs in cerebrospinal fluid as biomarker for disease course monitoring in primary central nervous system lymphoma. J Neurooncol 2012; 109: 239-244.
  19. Hsieh CC, Hsu HS, Chang SC, Chen YJ. Circulating Cell-Free DNA Levels Could Predict Oncological Outcomes of Patients Undergoing Esophagectomy for Esophageal Squamous Cell Carcinoma. Int J Mol Sci 2016; 17: 12.
  20. Kamel AM, Teama S, Fawzy A, El Deftar M. Plasma DNA integrity index as a potential molecular diagnostic marker for breast cancer. Tumour Biol 2016; 37: 7565-7572.
  21. Huang A, Zhang X, Zhou SL, Cao Y, Huang XW, Fan J, et al. Plasma Circulating Cell-free DNA Integrity as a Promising Biomarker for Diagnosis and Surveillance in Patients with Hepatocellular Carcinoma. J Cancer 2016; 7: 1798-1803.
  22. Yoruker EE, Ozgur E, Keskin M, Dalay N, Holdenrieder S, Gezer U. Assessment of circulating serum DNA integrity in colorectal cancer patients. Anticancer Res 2015; 35: 2435-2440.
  23. Cheng J, Cuk K, Heil J, Golatta M, Schott S, Sohn C, et al. Cell-free circulating DNA integrity is an independent predictor of impending breast cancer recurrence. Oncotarget 2017; 8: 54537-54547.
Read More
Author biographies is not available.
Crossref
Scopus
Google Scholar
Europe PMC
Download this PDF file
PDF
Statistic
Read Counter : 782 Download : 472