Development of dual priming oligonucleotide-polymerase chain reaction (DPO-PCR) for detection of wheat component in foods

Yan Su; Rong Li

doi:10.14715/cmb/2019.65.6.13

Issue

Vol. 65 No. 6: Issue 6

Issue Published : August 5, 2019

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Development of dual priming oligonucleotide-polymerase chain reaction (DPO-PCR) for detection of wheat component in foods

https://doi.org/10.14715/cmb/2019.65.6.13

Yan Su

Hefei Technology College, Hefei, 238000, China

Rong Li

Chaohu University, Chaohu, 238000, China

Corresponding Author(s) : Rong Li

vumdv8@163.com

Cellular and Molecular Biology, Vol. 65 No. 6: Issue 6
Article Published : July 31, 2019

Abstract

This research is aimed to establish a DPO-PCR method for rapid detection of wheat component in foods. A pair of highly specific DPO primers was designed using the wheat housekeeping gene GAG56D as the target gene. By optimizing the PCR reaction system, the DPO-PCR detection method for wheat component in food was established, and the specificity and sensitivity of the method were determined. The established DPO-PCR method was highly specific, and there was no cross-reactivity to 22 control samples. The sensitivity was high, and the minimum detection limit was 1 ng/uL. Tests on commercially available product samples showed that the method can effectively detect wheat component of foods. The DPO-PCR method established in this study is simple and accurate, and provides an effective detection method for wheat component in foods.

Keywords

Dual priming oligonucleotide polymerase chain reaction Wheat component Foods.

Su, Y., & Li, R. (2019). Development of dual priming oligonucleotide-polymerase chain reaction (DPO-PCR) for detection of wheat component in foods. Cellular and Molecular Biology, 65(6), 81–84. https://doi.org/10.14715/cmb/2019.65.6.13

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References

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Sicherer SH. Epidemiology of food allergy. J Allergy Clin Immunol 2011; 127: 594-602.
Branum AM, Lukacs SL. Food Allergy Among Children in the United States. Pediatr 2009; 124: 1549-1555.
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Keet CA, Matsui EC, Dhillon G, Lenehan P, Paterakis M, Wood RA. The natural history of wheat allergy. Ann Allergy Asthma Immunol 2009; 102: 410-415.
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Yano T, Sakai Y, Uchida K, Nakao Y, Ishihata K, Nakano S, et al. Detection of walnut residues in processed foods by polymerase chain reaction. Biosci Biotechnol Biochem 2007; 71: 1793-1796
Hirao T, Imai S, Sawad AH, Shiomi N, Hachimura S, Kato H. PCR method for detecting trace amounts of buck wheat (Fagopyrum spp.) in food. Biosci Biotechnol Biochem 2005; 69: 724-731.
Eischeid AC, Stadig SR. A group-specific, quantitative real-time PCR assay for detection of crab, a crustacean shellfish allergen, in complex food matrices. Food Chem 2018; 244: 224.
ífrica S, Ballesteros I, Marqués E, Dieguez MC, Rueda J, Cuadrado C, et al. Evaluation of locked nucleic acid and TaqMan probes for specific detection of cashew nut in processed food by real time PCR. Food Control 2018; 89: 227-234.
Costa J, Amaral JS, Grazina L, Oliveira M, Beatriz PP, Mafra Isabel. Matrix-normalised real-time PCR approach to quantify soybean as a potential food allergen as affected by thermal processing. Food Chem 2017; 221: 1843-1850.
Dan Y, Jilie K, Xinxin L, Fang X, Chen Q. Colorimetric LAMP microfluidic chip for detecting three allergens: peanut, sesame and soybean. Sci Rep 2018; 8: 8682-8685.
Garridomaestu A, Azinheiro S, Fuciños P, Carvalho J, Rodríguez MP. Highly sensitive detectionof gluten-containing cereals in food samples by real-time Loop-mediated isothermal AMPlification (qLAMP) and real-time polymerase chain reaction (qPCR). Food Chem 2018; 246: 156-163.
Chun JY, Kim KJ, Hwang IT, Kim YJ, Lee DH, Lee IK, et al. Dual priming oligonucleotide system for the multiplex detection of respiratory viruses and SNP genotyping of CYP2C19 gene. Nucleic Acids Res 2007; 35: 1706-1709.
Chung WC, Jeon EJ, Oh JH, Cheung DY, et al. Dual-priming oligonucleotide-based multiplex PCR using tissue samples from the rapid urease test kit for the detection of Helicobacter pylori in bleeding peptic ulcers. Park JM4, Kim TH 2016; 48: 899-903.
Lee HR, Kim SY, Chang HE, Song SH, Lee HS, Park KU, et al. Novel Multiplex PCR Using Dual-Priming Oligonucleotides for Detection and Discrimination of the Mycobacterium tuberculosis Complex and M. bovis BCG. J Clin Microbiol 2010; 48: 4612-4614.
Horii T, Ohtsuka H, Osaki M, Ohkuni H. Use of a dual priming oligonucleotide system to detect multiple sexually transmitted pathogens in clinical specimens. Lett Appl Microbiol 2009; 49: 46-52.
Xu YG, Liu ZM, Guan XT, Cui LC, Li SL. Dual priming oligonucleotide (DPO)-based multiplex PCR assay for specific detection of four diarrhoeagenic Escherichia coli in food. Lett Appl Microbiol 2015; 61: 146-152.

References

Sicherer SH, Sampson HA. Food allergy. J Allergy Clin Immunol 2010; 125: 116-125.

Sicherer SH. Epidemiology of food allergy. J Allergy Clin Immunol 2011; 127: 594-602.

Branum AM, Lukacs SL. Food Allergy Among Children in the United States. Pediatr 2009; 124: 1549-1555.

Codex Alimentarius Commission. General standard for the labelling of prepackaged foods (codex stan 1-1985, amendment.7-2010). 2010.

Keet CA, Matsui EC, Dhillon G, Lenehan P, Paterakis M, Wood RA. The natural history of wheat allergy. Ann Allergy Asthma Immunol 2009; 102: 410-415.

Inomata N. Wheat allergy. Curr Opin Allergy Clin Immunol 2009; 9: 238-243.

Scibilia J, Pastorello EA, Zisa G, Ottolenghi A, Bindslev-Jensen C, Pravettoni V, et al. Wheat allergy: A double-blind, placebo-controlled study in adults. J Allergy Clin Immunol 2006; 117: 433-439.

Poms RE, Klein CL, Anklam E. Methods for allergen analysis in food: a review. Food Addit Contam 2004; 21: 1-31.

Yano T, Sakai Y, Uchida K, Nakao Y, Ishihata K, Nakano S, et al. Detection of walnut residues in processed foods by polymerase chain reaction. Biosci Biotechnol Biochem 2007; 71: 1793-1796

Hirao T, Imai S, Sawad AH, Shiomi N, Hachimura S, Kato H. PCR method for detecting trace amounts of buck wheat (Fagopyrum spp.) in food. Biosci Biotechnol Biochem 2005; 69: 724-731.

Eischeid AC, Stadig SR. A group-specific, quantitative real-time PCR assay for detection of crab, a crustacean shellfish allergen, in complex food matrices. Food Chem 2018; 244: 224.

ífrica S, Ballesteros I, Marqués E, Dieguez MC, Rueda J, Cuadrado C, et al. Evaluation of locked nucleic acid and TaqMan probes for specific detection of cashew nut in processed food by real time PCR. Food Control 2018; 89: 227-234.

Costa J, Amaral JS, Grazina L, Oliveira M, Beatriz PP, Mafra Isabel. Matrix-normalised real-time PCR approach to quantify soybean as a potential food allergen as affected by thermal processing. Food Chem 2017; 221: 1843-1850.

Dan Y, Jilie K, Xinxin L, Fang X, Chen Q. Colorimetric LAMP microfluidic chip for detecting three allergens: peanut, sesame and soybean. Sci Rep 2018; 8: 8682-8685.

Garridomaestu A, Azinheiro S, Fuciños P, Carvalho J, Rodríguez MP. Highly sensitive detectionof gluten-containing cereals in food samples by real-time Loop-mediated isothermal AMPlification (qLAMP) and real-time polymerase chain reaction (qPCR). Food Chem 2018; 246: 156-163.

Chun JY, Kim KJ, Hwang IT, Kim YJ, Lee DH, Lee IK, et al. Dual priming oligonucleotide system for the multiplex detection of respiratory viruses and SNP genotyping of CYP2C19 gene. Nucleic Acids Res 2007; 35: 1706-1709.

Chung WC, Jeon EJ, Oh JH, Cheung DY, et al. Dual-priming oligonucleotide-based multiplex PCR using tissue samples from the rapid urease test kit for the detection of Helicobacter pylori in bleeding peptic ulcers. Park JM4, Kim TH 2016; 48: 899-903.

Lee HR, Kim SY, Chang HE, Song SH, Lee HS, Park KU, et al. Novel Multiplex PCR Using Dual-Priming Oligonucleotides for Detection and Discrimination of the Mycobacterium tuberculosis Complex and M. bovis BCG. J Clin Microbiol 2010; 48: 4612-4614.

Horii T, Ohtsuka H, Osaki M, Ohkuni H. Use of a dual priming oligonucleotide system to detect multiple sexually transmitted pathogens in clinical specimens. Lett Appl Microbiol 2009; 49: 46-52.

Xu YG, Liu ZM, Guan XT, Cui LC, Li SL. Dual priming oligonucleotide (DPO)-based multiplex PCR assay for specific detection of four diarrhoeagenic Escherichia coli in food. Lett Appl Microbiol 2015; 61: 146-152.

Author biographies is not available.