Issue

Vol. 66 No. 5: Issue 5

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

Diabetes up-regulated collagen IV and laminin α5 genes in mRNA and protein levels in seminiferous tubules of C57BL/6 adult mice

https://doi.org/10.14715/cmb/2020.66.5.28
Elnaz Khordad
Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Mohammad Reza Nikravesh
Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Mehdi Jalali
Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Ali Reza Fazel
Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Mojtaba Sankian
Bou-Ali Research Institute, Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Fatemeh Alipour
Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Corresponding Author(s) : Elnaz Khordad

khordade1@thums.ac.ir

Cellular and Molecular Biology, Vol. 66 No. 5: Issue 5

Abstract

Diabetes is a disease associated with impairment of the male reproductive system that causes complications such as decreased testosterone, the diameter of the seminiferous tubule, libido, and fertility. Extracellular matrix (ECM) molecules are involved in testicular development and spermatogenesis. Laminin and collagen are key proteins in seminiferous tubule basement membrane and play an important role in spermatogenesis. The present study was conducted to investigate the effect of diabetes on collagen IV and laminin α5 changes in mice testis. In this experimental study, 40 mice (C57BL/6) were divided randomly into 4 groups: 1) Control group: without intervention, 2) Diabetic group: treated mice with 50 mg/kg streptozotocin (STZ), 3) Diabetic + Insulin group: treated mice with STZ and insulin, and 4) Sham group: received citrate buffer. After 35 days, the left testes of all specimens were used for Real-Time PCR while their right testes were applied for immunohistochemical study and Periodic acid–Schiff (PAS) staining. This study showed that gene expression and immunoreactivity of laminin α5 and collagen IV were significantly increased in diabetic mice compared to other groups (P<0.05). Also, PAS staining showed the thickness of seminiferous tubule basement membrane in the Diabetic group compared to other group increased significantly (p<0.05). In Diabetic + Insulin compared to Diabetic group, gene expression, the intensity of immunoreactivity and thickness of seminiferous tubule basement membrane decreased significantly (P<0.05). Our findings indicated that diabetes causes up-regulation of collagen IV and laminin α5 in mRNA and protein levels in the seminiferous tubule basement membrane and may cause disorder in spermatogenesis in mice.

Keywords

Diabetes Testis Basement membrane Laminin α5 Collagen IV.
Khordad, E., Nikravesh, M. R., Jalali, M., Fazel, A. R., Sankian, M., & Alipour, F. (2020). Diabetes up-regulated collagen IV and laminin α5 genes in mRNA and protein levels in seminiferous tubules of C57BL/6 adult mice. Cellular and Molecular Biology, 66(5), 162–168. https://doi.org/10.14715/cmb/2020.66.5.28
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. Amini M, Shirinbayan P, Behnam B et al. Correlation between expression of CatSper family and sperm profiles in the adult mouse testis following Iranian Kerack abuse. Andrology. May 2014; 2(3): 386-393.
  2. Gispen WH, Biessels GJ. Cognition and synaptic plasticity in diabetes mellitus. Trends Neurosci. Nov 2000; 23(11): 542-549.
  3. Guneli E, Tugyan K, Ozturk H, Gumustekin M, Cilaker S, Uysal N. Effect of melatonin on testicular damage in streptozotocin-induced diabetes rats. Eur Surg Res. 2008; 40(4): 354-360.
  4. Sasso FC, De Nicola L, Carbonara O et al. Cardiovascular risk factors and disease management in type 2 diabetic patients with diabetic nephropathy. Diabetes Care. Mar 2006; 29(3): 498-503.
  5. Pourentezari M, Talebi AR, Mangoli E, Anvari M, Rahimipour M. Additional deleterious effects of alcohol consumption on sperm parameters and DNA integrity in diabetic mice. Andrologia. Jun 2016; 48(5): 564-569.
  6. Ballester J, Munoz MC, Dominguez J, Rigau T, Guinovart JJ, Rodriguez-Gil JE. Insulin-dependent diabetes affects testicular function by FSH- and LH-linked mechanisms. Journal of andrology. Sep-Oct 2004; 25(5): 706-719.
  7. Abdolahnejad A, Gol A, Sh D. Garlic effects on reproductive complications of diabetes mellitus imn male rats. Physio & Pharma. 2009; 12: 297-207.
  8. Salimnejad R, Sazegar G, Saeedi Borujeni MJ, Mousavi SM, Salehi F, Ghorbani F. Protective effect of hydroalcoholic extract of Teucrium polium on diabetes-induced testicular damage and serum testosterone concentration. International journal of reproductive biomedicine (Yazd, Iran). Apr 2017; 15(4): 195-202.
  9. Raychoudhury S, Raychoudhury K , Cl. M. Biotechnological evaluation of extracellular matrix proteins expressed by cultured testicular cells. Biotech. 2011; 3: 62-71.
  10. Luo ZJ, King RH, Lewin J, Thomas PK. Effects of nonenzymatic glycosylation of extracellular matrix components on cell survival and sensory neurite extension in cell culture. J Neurol. Apr 2002; 249(4): 424-431.
  11. Oshitari T, Polewski P, Chadda M, Li AF, Sato T, Roy S. Effect of combined antisense oligonucleotides against high-glucose- and diabetes-induced overexpression of extracellular matrix components and increased vascular permeability. Diabetes. Jan 2006; 55(1): 86-92.
  12. Soucy PA, Romer LH. Endothelial cell adhesion, signaling, and morphogenesis in fibroblast-derived matrix. Matrix Biol. Jun 2009; 28(5): 273-283.
  13. Yasuda H, Terada M, Maeda K et al. Diabetic neuropathy and nerve regeneration. Prog Neurobiol. Mar 2003; 69(4): 229-285.
  14. Davis CM, Papadopoulos V, Sommers CL, Kleinman HK, Dym M. Differential expression of extracellular matrix components in rat Sertoli cells. Biology of reproduction. Nov 1990; 43(5): 860-869.
  15. Gesing A, Lewinski A, Karbownik-Lewinska M. The thyroid gland and the process of aging; what is new? Thyroid research. Nov 24 2012; 5(1): 16.
  16. Gulkesen KH, Erdogru T, Sargin CF, Karpuzoglu G. Expression of extracellular matrix proteins and vimentin in testes of azoospermic man: an immunohistochemical and morphometric study. Asian journal of andrology. Mar 2002; 4(1): 55-60.
  17. Loveland K, Schlatt S, Sasaki T, Chu ML, Timpl R, Dziadek M. Developmental changes in the basement membrane of the normal and hypothyroid postnatal rat testis: segmental localization of fibulin-2 and fibronectin. Biology of reproduction. May 1998; 58(5): 1123-1130.
  18. Ulisse S, Rucci N, Piersanti D et al. Regulation by thyroid hormone of the expression of basement membrane components in rat prepubertal Sertoli cells. Endocrinology. Feb 1998; 139(2): 741-747.
  19. Yazama F, Esaki M, Sawada H. Immunocytochemistry of extracellular matrix components in the rat seminiferous tubule: electron microscopic localization with improved methodology. The Anatomical record. May 1997; 248(1): 51-62.
  20. Khoshnoodi J, Pedchenko V, Hudson BG. Mammalian collagen IV. Microsc Res Tech. May 2008; 71(5): 357-370.
  21. Patton BL. Laminins of the neuromuscular system. Microsc Res Tech. Nov 1 2000; 51(3): 247-261.
  22. Aldaghi M, Sankian M, Sameni H, Safari M. Effects of Alpha Lipoic Acid Treatment on Laminin Alteration of the Sciatic Nerve in Streptozotocin Induced Diabetic Rats. Middle East J Rehabil Health. 2014; 2.
  23. Pujar A, Pereira T, Tamgadge A, Bhalerao S, Tamgadge S. Comparing The Efficacy of Hematoxylin and Eosin, Periodic Acid Schiff and Fluorescent Periodic Acid Schiff-Acriflavine Techniques for Demonstration of Basement Membrane in Oral Lichen Planus: A Histochemical Study. Indian Journal of Dermatology. Sep-Oct 2015; 60(5): 450-456.
  24. Hadley MA, Byers SW, Suarez-Quian CA, Kleinman HK, Dym M. Extracellular matrix regulates Sertoli cell differentiation, testicular cord formation, and germ cell development in vitro. The Journal of cell biology. Oct 1985; 101(4): 1511-1522.
  25. Sahoo DK, Roy A, Bhanja S, Chainy GB. Hypothyroidism impairs antioxidant defence system and testicular physiology during development and maturation. General and comparative endocrinology. Mar 01 2008; 156(1): 63-70.
  26. Buzzard JJ, Morrison JR, O'Bryan MK, Song Q, Wreford NG. Developmental expression of thyroid hormone receptors in the rat testis. Biology of reproduction. Mar 2000; 62(3): 664-669.
  27. Wajner SM, Wagner MS, Maia AL. Clinical implications of altered thyroid status in male testicular function. Arquivos brasileiros de endocrinologia e metabologia. Nov 2009; 53(8): 976-982.
  28. Kanter M, Aktas C, Erboga M. Protective effects of quercetin against apoptosis and oxidative stress in streptozotocin-induced diabetic rat testis. Food Chem Toxicol. Mar 2012; 50(3-4): 719-725.
  29. Poulsom R, Kurkinen M, Prockop DJ, Boot-Handford RP. Increased steady-state levels of laminin B1 mRNA in kidneys of long-term streptozotocin-diabetic rats. No effect of an aldose reductase inhibitor. J Biol Chem. Jul 25 1988; 263(21): 10072-10076.
  30. Singh R, Hamada AJ, A A. Thyroid Hormones in Male Reproduction and Fertility. . The Open Reproductive Science Journal. 2011; 3: 98-104.
  31. Agbaje IM, Rogers DA, McVicar CM et al. Insulin dependant diabetes mellitus: implications for male reproductive function. Human reproduction (Oxford, England). Jul 2007; 22(7): 1871-1877.
  32. Alves MG, Martins AD, Rato L, Moreira PI, Socorro S, Oliveira PF. Molecular mechanisms beyond glucose transport in diabetes-related male infertility. Biochim Biophys Acta. May 2013; 1832(5): 626-635.
Read More
Author biographies is not available.
Crossref
Scopus
Google Scholar
Europe PMC
Download this PDF file
PDF
Statistic
Read Counter : 922 Download : 55

Most read articles by the same author(s)