Issue

Vol. 64 No. 7: Issue 7

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

Antioxidant capacity and phylogenetic analysis of twenty native grape cultivars in Siirt province, Turkey

https://doi.org/10.14715/cmb/2018.64.7.3
Mehmet Fidan
Biology Department, Science and Art Faculty, Siirt University, Siirt, Turkey
Mehmet Emre Erez
Biology Department, Science and Art Faculty, Siirt University, Siirt, Turkey
Behcet ć°nal
Agricultural Biotechnology Department, Agriculture Faculty, Siirt University, Siirt, Turkey
Süleyman Mesut Pinar
Van School of Healthy, Van Yüzüncü Yil University, Van, Turkey
Serdar Altintaş
Horticulture Department, Agriculture Faculty, Siirt University, Siirt, Turkey

Corresponding Author(s) : Mehmet Fidan

mfidan7384@hotmail.com

Cellular and Molecular Biology, Vol. 64 No. 7: Issue 7

Abstract

The quality of grape cultivars not only depends on the grape cultivar but also is influenced by the molecular concepts and agro-climatic factors. For this purpose, four different grape cultivars were collected from five different locations in Siirt province (Turkey). Totally twenty different grape cultivars were investigated. In the present study, the antioxidant activity (total phenolic, flavonoid, proanthocyanidin content, DPPH and FRAP activity) in seeds were indicated and phylogenetic analysis (cpDNA;trnL-F region) of twenty native grape cultivars were investigated to construct their phylogenetic tree. According to reported data on antioxidant activity and content of phytochemicals, all cultivars exhibited different values from each other, but Rutik and Gadüv cultivars were found as significantly higher in comparison to others. According to bioinformatics analysis, twenty grape cultivars were distributed into six different major groups. Rutik and Sevkeye cultivars exhibit significant distinction from other grape cultivars. The phylogenetic analysis was also associated and supported with the results of obtained data from bioactivity. The bioactivity and phylogenetic analysis were firstly identified and quantified in these grape cultivars, however, with regard to obtained data from the current study, the grape cultivars grown in Siirt province were indicated significant and valuable results and as a result, these cultivars have to be evaluated before extinction.

Keywords

ine cultivars Biological activity Genetic relationship Siirt Turkey.
Fidan, M., Erez, M. E., ć°nal, B., Pinar, S. M., & Altintaş, S. (2018). Antioxidant capacity and phylogenetic analysis of twenty native grape cultivars in Siirt province, Turkey. Cellular and Molecular Biology, 64(7), 14–18. https://doi.org/10.14715/cmb/2018.64.7.3
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. Casazza AA, Aliakbarian B, Mantegna S, Cravotto G, Perego P. Extraction of phenolics from Vitis vinifera wastes using non-conventional techniques. Journal of Food Engineering 2010; 100:50-55.
  2. Guendez R, Kallithraka S, Makris DP, Kefalas P. Determination of low molecular weight polyphenolic constituents in grape (Vitis vinifera sp.) seed extracts: Correlation with antiradical activity. Food Chemistry 2005; 89:1-9.
  3. Yilmaz Y, Toledo RT. Oxygen radical absorbance capacities of grape/wine industry byproducts and effect of solvent type on extraction of grape seed polyphenols. Journal of Food Composition and Analysis 2006; 19:41-48.
  4. Xu C, Zhang Y, Wang J, Lu J. Extraction, distribution and characterisation of phenolic compounds and oil in grapeseeds. Food chemistry 2010; 122:688-694.
  5. Rusjan D, Veberić R, Mikulić-Petkovšek M. The response of phenolic compounds in grapes of the variety ‘Chardonnay'(Vitis vinifera L.) to the infection by phytoplasma Bois noir. European journal of plant pathology 2012; 133:965-974.
  6. Figueiredo A, Fortes AM, Ferreira S, Sebastiana M, Choi YH, Sousa L, Acioli-Santos B, Pessoa F, Verpoorte R, Pais MS. Transcriptional and metabolic profiling of grape (Vitis vinifera L.) leaves unravel possible innate resistance against pathogenic fungi. Journal of experimental Botany 2008; 59:3371-3381.
  7. Gutha LR, Casassa LF, Harbertson JF, Naidu RA. Modulation of flavonoid biosynthetic pathway genes and anthocyanins due to virus infection in grapevine (Vitis vinifera L.) leaves. BMC Plant Biology 2010; 10:187.
  8. Hren M, Nikolić P, Rotter A, Blejec A, Terrier N, Ravnikar M, Dermastia M, Gruden K. 'Bois noir'phytoplasma induces significant reprogramming of the leaf transcriptome in the field grown grapevine. BMC genomics 2009; 10:460.
  9. Aoki K, Matsumura T, Hattori T, Murakami N. Chloroplast DNA phylogeography of Photinia glabra (Rosaceae) in Japan. American journal of botany 2006; 93:1852-1858.
  10. Slinkard K, Singleton VL. Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture 1977; 28:49-55.
  11. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food chemistry 1999; 64:555-559.
  12. Zou Y, Lu Y, Wei D. Antioxidant activity of a flavonoid-rich extract of Hypericum perforatum L. in vitro. Journal of Agricultural and Food Chemistry 2004; 52:5032-5039.
  13. Villano D, Fernández-Pachón M, Moyá M, Troncoso A, Garcí­a-Parrilla M. Radical scavenging ability of polyphenolic compounds towards DPPH free radical. Talanta 2007; 71:230-235.
  14. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power”: the FRAP assay. Analytical biochemistry 1996; 239:70-76.
  15. Prior RL, Fan E, Ji H, Howell A, Nio C, Payne MJ, Reed J. Multi"laboratory validation of a standard method for quantifying proanthocyanidins in cranberry powders. Journal of the Science of Food and Agriculture 2010; 90:1473-1478.
  16. Doyle J, Doyle J. Genomic plant DNA preparation from fresh tissue-CTAB method. Phytochem Bull 1987; 19:11-15.
  17. Karaca M, ć°nce AG, Elmasulu SY, Onus AN, Turgut K. Coisolation of genomic and organelle DNAs from 15 genera and 31 species of plants. Analytical biochemistry 2005; 343:353-355.
  18. Taberlet P, Gielly L, Pautou G, Bouvet J. Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant molecular biology 1991; 17:1105-1109.
  19. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic acids research 1994; 22:4673-4680.
  20. Karababa E, Develi Isikli N. Pekmez: a traditional concentrated fruit product. Food Reviews International 2005; 21:357-366.
  21. Santos JA, Malheiro AC, Karremann MK, Pinto JG. Statistical modelling of grapevine yield in the Port Wine region under present and future climate conditions. International Journal of Biometeorology 2011; 55:119-131.
  22. í‡etin ES, Altinöz D, Tarçan E, Baydar NG. Chemical composition of grape canes. Industrial Crops and Products 2011; 34:994-998.
  23. Shi J, Yu J, Pohorly JE, Kakuda Y. Polyphenolics in grape seeds”biochemistry and functionality. Journal of medicinal food 2003; 6:291-299.
  24. Rockenbach II, Gonzaga LV, Rizelio VM, Gonçalves AEdSS, Genovese MI, Fett R. Phenolic compounds and antioxidant activity of seed and skin extracts of red grape (Vitis vinifera and Vitis labrusca) pomace from Brazilian winemaking. Food Research International 2011; 44:897-901.
  25. Montealegre RR, Peces RR, Vozmediano JC, Gascueña JM, Romero EG. Phenolic compounds in skins and seeds of ten grape Vitis vinifera varieties grown in a warm climate. Journal of Food Composition and Analysis 2006; 19:687-693.
  26. Bartolome B, Nunez V, Monagas M, Gómez-Cordovés C. In vitro antioxidant activity of red grape skins. European Food Research and Technology 2004; 218:173-177.
  27. Louli V, Ragoussis N, Magoulas K. Recovery of phenolic antioxidants from wine industry by-products. Bioresource technology 2004; 92:201-208.
  28. Liu X-Q, Ickert-Bond SM, Chen L-Q, Wen J. Molecular phylogeny of Cissus L. of Vitaceae (the grape family) and evolution of its pantropical intercontinental disjunctions. Molecular phylogenetics and evolution 2013; 66:43-53.
  29. Rossetto M, Jackes BR, Scott KD, Henry RJ. Intergeneric relationships in the Australian Vitaceae: new evidence from cpDNA analysis. Genetic Resources and Crop Evolution 2001; 48:307-314.
  30. Zhang N, Wen J, Zimmer EA. Correction: Congruent Deep Relationships in the Grape Family (Vitaceae) Based on Sequences of Chloroplast Genomes and Mitochondrial Genes via Genome Skimming. PloS one 2016; 11:e0152059.
  31. Adrian M, Jeandet P, Bessis R, Joubert J. Induction of phytoalexin (resveratrol) synthesis in grapevine leaves treated with aluminum chloride (AlCl3). Journal of Agricultural and Food Chemistry 1996; 44:1979-1981.
Read More
Author biographies is not available.
Crossref
Scopus
Google Scholar
Europe PMC
Download this PDF file
PDF
Statistic
Read Counter : 1073 Download : 535