Bioactive flavones isolated from Tunisian Artemisia campestris L. Leaves

Rafika Metoui; Jalloul Bouajila; Mansour Znati; Sylvie Cazaux; Mohamed Neffati; Ahmed Akrout

doi:10.14715/cmb/2017.63.11.15

Issue

Vol. 63 No. 11: Issue 11

Issue Published : November 30, 2017

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Bioactive flavones isolated from Tunisian Artemisia campestris L. Leaves

https://doi.org/10.14715/cmb/2017.63.11.15

Rafika Metoui

Range Ecology Laboratory, University of Gabès, Institute of Arid Lands, 4119 Medenine, Tunisia

Jalloul Bouajila

Laboratory of IMRCP UMR CNRS 5623, Faculty of Pharmacy of Toulouse, University of Toulouse, University of Paul-Sabatier, F-31062 Toulouse, France

Mansour Znati

Laboratory of IMRCP UMR CNRS 5623, Faculty of Pharmacy of Toulouse, University of Toulouse, University of Paul-Sabatier, F-31062 Toulouse, France

Sylvie Cazaux

Laboratory of IMRCP UMR CNRS 5623, Faculty of Pharmacy of Toulouse, University of Toulouse, University of Paul-Sabatier, F-31062 Toulouse, France

Mohamed Neffati

Range Ecology Laboratory, University of Gabès, Institute of Arid Lands, 4119 Medenine, Tunisia

Ahmed Akrout

Range Ecology Laboratory, University of Gabès, Institute of Arid Lands, 4119 Medenine, Tunisia

Corresponding Author(s) : Ahmed Akrout

ahmed.akrout@ira.rnnt.tn

Cellular and Molecular Biology, Vol. 63 No. 11: Issue 11
Article Published : November 30, 2017

Abstract

Four flavones were isolated from dried leaves of Artemisia campestris L. 2',4',5,7-tetrahydroxy-5',6-dimethoxyflavone, eupatilin and dimethoxycentaureidin were reported for the first time in this species whereas cirsiliol was previously identified but it was isolated for the first time. Their structures were elucidated by spectroscopic methods, including 1D and 2D NMR experiments and mass spectrometry analysis. In addition, all isolated flavones were evaluated for their antioxidant, anti-inflammatory, anti-superoxide dismutase, anti-xanthine oxidase and cytotoxic activities. The results showed that all isolated compounds exhibited potent anti-xanthine oxidase activity with IC50 ranging from 3.3 to 6.8 µM, which was higher than that of the control compound allopurinol (8.2 ± 0.6 µM). In addition, cirsiliol was found to be the most cytotoxic against OVCAR-3, IGROV-1and HCT-116 cell lines at 15µM, with inhibition percentage values of 53.7, 48.8 and 40.9%, respectively. All compounds also showed weak to moderate anti-inflammatory and anti-superoxide dismutase activities.

Keywords

Artemisia campestris Anti-inflammatory Anti-superoxide dismutase Anti-xanthine oxidase Cytotoxic activity.

Metoui, R., Bouajila, J., Znati, M., Cazaux, S., Neffati, M., & Akrout, A. (2017). Bioactive flavones isolated from Tunisian Artemisia campestris L. Leaves. Cellular and Molecular Biology, 63(11), 86–91. https://doi.org/10.14715/cmb/2017.63.11.15