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Vol. 63 No. 10: Issue 10

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Interleukin-6 secreted by oral cancer- associated fibroblast accelerated VEGF expression in tumor and stroma cells

https://doi.org/10.14715/cmb/2017.63.10.21
M. Mirkeshavarz
Department of Oral and Maxillofacial Pathology, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
M. Ganjibakhsh
Human and Animal cell Bank, Iranian Biological Resource Center (IBRC), ACECR, Tehran, Iran
P. Aminishakib
Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
P. Farzaneh
Human and Animal cell Bank, Iranian Biological Resource Center (IBRC), ACECR, Tehran, Iran
N. Mahdavi
Department of Oral and Maxillofacial Pathology, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
F. Vakhshiteh
Human and Animal cell Bank, Iranian Biological Resource Center (IBRC), ACECR, Tehran, Iran
A. Karimi
Department of Oral and Maxillofacial Surgery, Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran
N. S. Gohari
Human and Animal cell Bank, Iranian Biological Resource Center (IBRC), ACECR, Tehran, Iran
F. Kamali
Tumor Bank, Cancer Institute Hospital, Tehran University of Medical Sciences, Tehran, Iran
M. J. Kharazifard
Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
S. A. Shahzadeh Fazeli
Human and Animal cell Bank, Iranian Biological Resource Center (IBRC), ACECR, Tehran, Iran
A. Nasimian
Human and Animal cell Bank, Iranian Biological Resource Center (IBRC), ACECR, Tehran, Iran

Corresponding Author(s) : A. Nasimian

Ahmad.nasimian@ibrc.ir

Cellular and Molecular Biology, Vol. 63 No. 10: Issue 10

Abstract

Oral cancer represents the sixth most common cancer type worldwide. Patients with oral cancer express high levels of IL-6 which is associated with very poor prognosis. Previous studies illustrated that IL-6 cytokine induces angiogenesis. It has also been reported that the presence of Cancer- Associated Fibroblasts (CAFs) is essential for angiogenesis. In this study, we examined the correlation between IL-6 and CAF and the role of this correlation on VEGF production. In this study, quantitative expression level of IL-6 and VEGF in CAF and Oral Cancer Cells (OCCs) examined through Real Time PCR and ELISA and western blot analysis. In addition, maintenance and retention of IL-6 and VEGF checked out in co-culture experiment of CAF and OCC cells. These experiments demonstrated that in oral cancer, CAF cell line secretes significantly more IL-6 than OCC. Also IL-6 is a factor that causes VEGF secretion in CAF cell line. CAF is the basic and the most essential source for producing IL-6 in patients with oral cancer. Secreted IL-6 is able to induce VEGF production in both CAF and OCCs. Correlation between CAF, IL-6 and VEGF could be considered as an approach for cancer therapy.

Keywords

Interleukin-6 Cancer- Associated fibroblast VEGF Oral cancer cells Normal fibroblast.
Mirkeshavarz, M., Ganjibakhsh, M., Aminishakib, P., Farzaneh, P., Mahdavi, N., Vakhshiteh, F., Karimi, A., Gohari, N. S., Kamali, F., Kharazifard, M. J., Shahzadeh Fazeli, S. A., & Nasimian, A. (2017). Interleukin-6 secreted by oral cancer- associated fibroblast accelerated VEGF expression in tumor and stroma cells. Cellular and Molecular Biology, 63(10), 131–136. https://doi.org/10.14715/cmb/2017.63.10.21
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