cDNA sequencing and expression analysis of Dicentrarchus labrax heme oxygenase-1
Corresponding Author(s) : N. Prevot-D'Alvise
Cellular and Molecular Biology,
Vol. 54 No. 2: General Papers
The liver cDNA encoding heme oxygenase-1 (HO-1) was sequenced from European sea bass (Dicentrarchus labrax) (accession number no. EF139130). The HO-1 cDNA was 1250 bp in nucleotide length and the open reading frame encoded 277 amino acid residues. The deduced amino acid sequence of the European sea bass had 75% and 50% identity with the amino acid sequences of tetraodontiformes (Tetraodon nigroviridis and Takifugu rubripes) and human HO-1 proteins, respectively. A short hydrophobic transmembrane domain at the C-terminal region was found, and four histidine residues were highly conserved, including human his25 that is essential for HO catalytic activity. RT-PCR of mRNA from eight different European sea bass tissues revealed that, in a homeostatis state, the heme oxygenase-1 was abundant in the spleen and liver but not in the brain.
Heme oxygenase-1 cDNA European sea bass Dicentrarchus labrax cloning tissue expression.
Prevot-D’Alvise, N., Pierre, S., Gaillard, S., Gouze, E., Gouze, J. N., Aubert, J., Richard, S., & Grillasca, J. P. (2008). cDNA sequencing and expression analysis of Dicentrarchus labrax heme oxygenase-1. Cellular and Molecular Biology, 54(2), 1046–54. Retrieved from https://cellmolbiol.org/index.php/CMB/article/view/1027
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