cDNA sequencing and expression analysis of Dicentrarchus labrax heme oxygenase-1

N. Prevot-D'Alvise; S. Pierre; S. Gaillard; E. Gouze; J. N. Gouze; J. Aubert; S. Richard; J. P. Grillasca

Issue

Vol. 54 No. 2: General Papers

Issue Published : July 1, 2015

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

cDNA sequencing and expression analysis of Dicentrarchus labrax heme oxygenase-1

N. Prevot-D'Alvise

S. Pierre

S. Gaillard

E. Gouze

J. N. Gouze

J. Aubert

S. Richard

J. P. Grillasca

Corresponding Author(s) : N. Prevot-D'Alvise

nathalie.prevot@univ-tln.fr

Cellular and Molecular Biology, Vol. 54 No. 2: General Papers
Article Published : November 17, 2008

Abstract

The liver cDNA encoding heme oxygenase-1 (HO-1) was sequenced from European sea bass (Dicentrarchus labrax) (accession number no. EF139130). The HO-1 cDNA was 1250 bp in nucleotide length and the open reading frame encoded 277 amino acid residues. The deduced amino acid sequence of the European sea bass had 75% and 50% identity with the amino acid sequences of tetraodontiformes (Tetraodon nigroviridis and Takifugu rubripes) and human HO-1 proteins, respectively. A short hydrophobic transmembrane domain at the C-terminal region was found, and four histidine residues were highly conserved, including human his25 that is essential for HO catalytic activity. RT-PCR of mRNA from eight different European sea bass tissues revealed that, in a homeostatis state, the heme oxygenase-1 was abundant in the spleen and liver but not in the brain.

Keywords

Heme oxygenase-1 cDNA European sea bass Dicentrarchus labrax cloning tissue expression.

Prevot-D’Alvise, N., Pierre, S., Gaillard, S., Gouze, E., Gouze, J. N., Aubert, J., Richard, S., & Grillasca, J. P. (2008). cDNA sequencing and expression analysis of Dicentrarchus labrax heme oxygenase-1. Cellular and Molecular Biology, 54(2), 1046–54. Retrieved from https://cellmolbiol.org/index.php/CMB/article/view/1027