Molecular Regulation of Organic Anion Transporting Polypeptide 1A2 (OATP1A2)by Taurocholic Acid in Bewo Cells
Corresponding Author(s) : X. H. Yang
Cellular and Molecular Biology,
Vol. 60 No. 2: Issues 2
To characterize the mechanisms of action of taurocholic acid(TCA) and farnesoid X receptor(FXR) on organic anion transporting polypeptide 1A2(OATP1A2) expression in placental Bewo cell line. Quantitative real-time PCR and Western blots were used to detect OATP1A2 in Bewo cells cultured with TCA and pcDNA3.1(+)-hFXR transfected Bewo cells after incubation with 2mM TCA for 48 hours. TCA(0.02mM) induced the mRNA and protein expression of OATP1A2 by 3 and 1.6 fold (p<0.05), respectively, while 0.2 and 2mM TCA induced mRNA and protein expression by 1.5 and 1.3 fold, respectively. The concentration of TCA was negatively correlated with OATP1A2 gene expression (P<0.05). In pcDNA3.1(+)-hFXR transfected Bewo cells with 2mM TCA demonstrated a 2-3 fold increase in OATP1A2 over controls (P<0.05). TCA is one of the regulation factors for OATP1A2 in the Bewo cell line. A low dose of TCA can induce fetal membrane expression of OATP1A2. This may present a physiological or compensatory mechanism of the placenta, while the high dose of TCA may produce a pathological or pathogenic mechanism. Farnesoid X receptor may act in synergy with TCA to increase the expression of OATP1A2. This may be a treatment strategy for fetal cholestasis.
Taurocholic acid Bewo cells OATP1A2 FXR bile acids transporter fetal cholestasis ICP.
Yang, X. H., Liu, S. Y., & Xing, A. Y. (2014). Molecular Regulation of Organic Anion Transporting Polypeptide 1A2 (OATP1A2)by Taurocholic Acid in Bewo Cells. Cellular and Molecular Biology, 60(2), 22–26. Retrieved from https://cellmolbiol.org/index.php/CMB/article/view/531
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