Ninghuang  Dai; Haitao Ma; Yu  Feng

doi:10.14715/cmb/2023.69.9.29

Issue

Vol. 69 No. 9: Issue 9

Issue Published : October 8, 2023

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Silencing of long non-coding RNA SDCBP2-AS1/microRNA-656-3p/CRIM1 axis promotes ferroptosis of lung cancer cells

LncRNA SDCBP2-AS1 affects ferroptosis of lung cancer cells

https://doi.org/10.14715/cmb/2023.69.9.29

Ninghuang Dai

Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, China

Haitao Ma

Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, China

Yu Feng

Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, China

Corresponding Author(s) : Haitao Ma

haitao_ma@publisher.org.cn

Cellular and Molecular Biology, Vol. 69 No. 9: Issue 9
Article Published : September 30, 2023

Abstract

Long non-coding RNAs (lncRNAs) play central roles in lung cancer progression by acting as competing endogenous RNAs (ceRNAs). This study aimed to explore the roles of lncRNA SDCBP2-AS1 in lung cancer and the molecular mechanism. The expression of SDCBP2-AS1, microRNA (miR)-656-3p, and cysteine-rich transmembrane BMP regulator 1 (CRIM1) was measured using quantitative real-time polymerase chain reaction. Ferroptosis was evaluated by analyzing cell death, ferrous content, reactive oxygen species (ROS) level, and protein levels of ferroptosis markers. The binding relationship was assessed using a dual-luciferase reporter assay. We observed that SDCBP2-AS1 was highly expressed in lung cancer cells. Knockdown of SDCBP2-AS1 promoted ferroptosis of lung cancer cells. SDCBP2-AS1 is a sponge of miR-656-3p, which directly targets CRIM1. Rescue experiments confirmed that SDCBP2-AS1 regulates ferroptosis by miR-656-3p, and overexpression of CRIM1 abrogated the effects of miR-656-3p on ferroptosis. In conclusion, depletion of SDCBP2-AS1 promoted lung cancer cell ferroptosis via the miR-656-3p/CRIM1 axis.

Keywords

lncRNA SDCBP2-AS1 lung cancer ferroptosis miR-656-3p CRIM1

Dai, N. ., Ma, H., & Feng, Y. . (2023). Silencing of long non-coding RNA SDCBP2-AS1/microRNA-656-3p/CRIM1 axis promotes ferroptosis of lung cancer cells: LncRNA SDCBP2-AS1 affects ferroptosis of lung cancer cells. Cellular and Molecular Biology, 69(9), 189–194. https://doi.org/10.14715/cmb/2023.69.9.29