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Vol. 66 No. 5: Issue 5

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Down-regulation of miR-556-3p inhibits hemangioma cell proliferation and promotes apoptosis by targeting VEGFC

https://doi.org/10.14715/cmb/2020.66.5.34
Wen Jin
Department of Medical Nursing, Tongling polytechnic College, China
Liang Chen
Department of hyperbaric oxygen, The Second People's Hospital of Hefei, China
Fengyun Gao
Department of Medical Nursing, Tongling polytechnic College, China
Mei Yang
Department of Medical Nursing, Tongling polytechnic College, China
Yan Liu
Department of Medical Nursing, Tongling polytechnic College, China
Bo Wang
Department of Medical Nursing, Tongling polytechnic College, China

Corresponding Author(s) : Bo Wang

jia1982yun@163.com

Cellular and Molecular Biology, Vol. 66 No. 5: Issue 5

Abstract

The purpose of this study was to determine the effect of microRNA (miR)-556-3p on cell proliferation and apoptosis of hemangioma-derived endothelial cells (HemECs).  qRT-PCR was used to compare the expressions of miR-556-3p in HemECs and normal cells. The target gene was identified using dual-luciferase reporter assay. Cell proliferation was measured with MTT assay, while western blotting was used to assay VEGFC expression levels. Apoptosis was assayed with FITC Annexin V Apoptosis assay kit. miR-556-3p was overexpressed in HemECs. Transfection with miR-556-3p inhibitor resulted in decreased proliferation of HemECs (p<0.05). The expression of the target gene of miR-556-3pi.e. VEGFC was upregulated when miR-556-3p inhibitor was transfected. The transfection also resulted in increased apoptosis. In HemECs, miR-556-3p is overexpressed and VEGFC expression is low. Thus, miR-556-3p or VEGFC might be potential targets for treatment of angiosarcoma.

Keywords

Angiosarcoma HemECs miR-556-3p VEGFC Proliferation Apoptosis.
Jin, W., Chen, L., Gao, F., Yang, M., Liu, Y., & Wang, B. (2020). Down-regulation of miR-556-3p inhibits hemangioma cell proliferation and promotes apoptosis by targeting VEGFC. Cellular and Molecular Biology, 66(5), 204–207. https://doi.org/10.14715/cmb/2020.66.5.34
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