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Vol. 65 No. 3: Issue 3

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Relationship between the methylation levels of Twist gene and pathogenesis of endometriosis

https://doi.org/10.14715/cmb/2019.65.3.14
Li Juanqing
Gynecology Department, Women's Hospital School of Medicine Zhejiang University, Xueshi Road 1st, Zhejiang Hangzhou 310006, China
Yu Hailan
Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital School of Medicine Zhejiang University, Qingchun Road 3rd, Zhejiang Hangzhou 310016, China
Fei Xiangwei
Gynecology Department, Women's Hospital School of Medicine Zhejiang University, Xueshi Road 1st, Zhejiang Hangzhou 310006, China
Zhang Tao
Gynecology Department, Women's Hospital School of Medicine Zhejiang University, Xueshi Road 1st, Zhejiang Hangzhou 310006, China
Ma Junyan
Gynecology Department, Women's Hospital School of Medicine Zhejiang University, Xueshi Road 1st, Zhejiang Hangzhou 310006, China
Zhou Jianhong
Gynecology Department, Women's Hospital School of Medicine Zhejiang University, Xueshi Road 1st, Zhejiang Hangzhou 310006, China
Lin Jun
Gynecology Department, Women's Hospital School of Medicine Zhejiang University, Xueshi Road 1st, Zhejiang Hangzhou 310006, China
Yang Jianhua
Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital School of Medicine Zhejiang University, Qingchun Road 3rd, Zhejiang Hangzhou 310016, China

Corresponding Author(s) : Yang Jianhua

zrtazz3@163.com

Cellular and Molecular Biology, Vol. 65 No. 3: Issue 3

Abstract

The purpose of this study was to investigate the difference of Twist gene promoter methylation among ovarian ectopic endometrium, eutopic endometrium and non-endometriosis (EMs) endometrium . 15 patients with reproductive age hospitalized at Department of obstetrics and gynecology affiliated to Medicine School of Zhejiang University from January 2013 to June 2016 were selected. Among them, 5 patients underwent laparoscopic surgery due to ovarian type EMs, and were selected after histologic confirmation. Ectopic endometrium and eutopic endometrium were obtained simultaneously. Normal endometrium was obtained from 5 cases of tubal infertility confirmed by hysteroscopy. Six pairs of primers for CpG island of Twist gene promoter were designed, and the difference of promoter methylation levels was detected by pyrosequencing method for methylation specific PCR (MSP) in three groups of endometrial tissues. The promoter of Twist gene is hypomethylated in some areas of ovarian ectopic endometrium and eutopic endometrium of ovarian endometriosis. It is speculated that the regional hypomethylation of Twist gene promoter in ovarian ectopic endometrium and eutopic endometrium may cause over-expression of Twist protein, which may directly lead to the pathogenesis of endometriosis.

Keywords

Twist Ectopic endometrium Eutopic endometrium Non-endometriosis (EMs) endometrium.
Juanqing, L., Hailan, Y., Xiangwei, F., Tao, Z., Junyan, M., Jianhong, Z., Jun, L., & Jianhua, Y. (2019). Relationship between the methylation levels of Twist gene and pathogenesis of endometriosis. Cellular and Molecular Biology, 65(3), 94–100. https://doi.org/10.14715/cmb/2019.65.3.14
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