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Vol. 64 No. 15: Issue 15

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Effects of Leontice leontopetalum and Bongardia chrysogonum on oxidative stress and neuroprotection in PTZ kindling epilepsy in rats

https://doi.org/10.14715/cmb/2017.64.15.12
Ozlem Ergul Erkec
Department of Physiology, Faculty of Medicine, Van Yuzuncu Yil University, Van, Turkey
Okan Arihan
Department of Physiology, Faculty of Medicine, Van Yuzuncu Yil University, Van, Turkey
Mehmet Kara
Department of Physiology, Faculty of Medicine, Van Yuzuncu Yil University, Van, Turkey
Ersin Karatas
Department of Medical Biochemistry, Faculty of Medicine, Bezmialem Vakif University, ć°stanbul, Turkey
Remzi Erten
Department of Pathology, Faculty of Medicine, Van Yuzuncu Yil University, Van, Turkey
Halit Demir
Department of Chemistry, Faculty of Science, Van Yuzuncu Yil University, Van, Turkey
Ismail Meral
Department of Physiology, Faculty of Medicine, Bezmialem Vakif University, Istanbul, Turkey
Muzaffer Mukemre
Department of Biology, Faculty of Science, Van Yuzuncu Yil University, Van, Turkey
Fevzi Ozgokce
Department of Biology, Faculty of Science, Van Yuzuncu Yil University, Van, Turkey

Corresponding Author(s) : Ozlem Ergul Erkec

oerkec@gmail.com

Cellular and Molecular Biology, Vol. 64 No. 15: Issue 15

Abstract

We investigated the effects of Leontice leontopetalum and Bongardia chrysogonum on apoptosis, gamma-aminobutyric acid (GABAA) receptor positive cell number, cyclin-B1 and bcl-2 levels and oxidative stress in pentylenetetrazol (PTZ) kindling in rats. Kindling was produced by subconvulsant doses of PTZ treatments in rats. Wistar albino rats were divided into 4 groups; Control, PTZ treated (PTZ), PTZ+L. leontopetalum extract treated (PTZ+LLE) and PTZ+B. chrysogonum extract treated (PTZ+BCE) groups. Extracts were given a dose (200 mg/kg) 2h before each PTZ injection. PTZ treatment significantly decreased the glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) activities and bcl-2 levels and increased the total oxidant status (TOS), malondialdehyde (MDA), cyclin B1, oxidative stress index (OSI) and number of neurons that expressed GABAA receptors when compared to the control. LLE and BCE possessed antioxidant activity in the brain and ameliorated PTZ induced oxidative stress, decreased cyclin-B1, increased bcl-2 levels, and kept the GABAA receptor number similar to that of the control despite the PTZ application.

Keywords

Bcl-2 Brain Bongardia chrysogonum Cyclin-B1 GABAA kindling model Leontice leontopetalum Oxidative stress Pentylenetetrazol.
Ergul Erkec, O., Arihan, O., Kara, M., Karatas, E., Erten, R., Demir, H., Meral, I., Mukemre, M., & Ozgokce, F. (2018). Effects of Leontice leontopetalum and Bongardia chrysogonum on oxidative stress and neuroprotection in PTZ kindling epilepsy in rats. Cellular and Molecular Biology, 64(15), 71–77. https://doi.org/10.14715/cmb/2017.64.15.12
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