Callus induction and somatic embryogenesis in Stevia rebaudiana Bertoni as a medicinal plant

Tahereh Keshvari; Abdollah Najaphy; Danial Kahrizi; Alireza Zebarjadi

doi:10.14715/cmb/2018.64.2.9

Issue

Vol. 64 No. 2: New biotechnological aspects in Stevia rebaudiana Bertoni

Issue Published : February 11, 2018

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Callus induction and somatic embryogenesis in Stevia rebaudiana Bertoni as a medicinal plant

https://doi.org/10.14715/cmb/2018.64.2.9

Tahereh Keshvari

Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran

Abdollah Najaphy

Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran

Danial Kahrizi

Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran

Alireza Zebarjadi

Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran

Corresponding Author(s) : Abdollah Najaphy

nadjaphy@yahoo.com

Cellular and Molecular Biology, Vol. 64 No. 2: New biotechnological aspects in Stevia rebaudiana Bertoni
Article Published : February 10, 2018

Abstract

Stevia rebaudiana (Bert.) from Asteraceae family is a useful medicinal plant that prevents and cures diabetes, blood pressure, weight gain and tooth decay. Due to self-incompatibility in stevia, somatic embryo investigation for artificial seed production is valuable in this plant. In order to evaluate the callus induction characteristics in stevia, a factorial experiment was laid out based on a completely randomized design with three replications. The factors included ten hormone combinations and control, two kinds of media (MS and B5) and two types of explants (leaf and internode). Callus induction characters including the percentage of callus formation, days to callus induction, fresh and dry callus weight were recorded. Analysis of variance showed significant differences (p<0.01) among hormone combinations, media and explant types as well as their interactions. The best treatment for callus induction with minimum time to callus formation was 1 mg/l NAA+1 mg/l BAP. The highest fresh and dry callus weight were obtained on B5 medium supplemented by 1 mg/l 2,4-D+1 mg/l BAP (in leaf explant) and 0.25 mg/l 2,4-D+ 0.1 mg/l BAP (in internode explant). These results can be used in suspension culture. To induce somatic embryogenesis in suspension culture, six hormone treatments were investigated. The highest somatic embryogenesis percentage was obtained in MS medium supplemented by 2 mg/l 2,4-D+ 0.5 mg/l NAA+0.5 mg/l BAP.

Keywords

Artificial Seed Callus Somatic Embryo Stevia Tissue Culture.

Keshvari, T., Najaphy, A., Kahrizi, D., & Zebarjadi, A. (2018). Callus induction and somatic embryogenesis in Stevia rebaudiana Bertoni as a medicinal plant. Cellular and Molecular Biology, 64(2), 46–49. https://doi.org/10.14715/cmb/2018.64.2.9

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References

Esmaeili F, Kahrizi D, Mansouri M, Yari K., Kazemi, N, Ghaheri, M. Cell dedifferentiation in Stevia rebauiana as a pharmaceutical and medicinal plant. J Rep Pharmaceutic Sci, 2016; 5(1): 12-17.
Ghorbani T, Kahrizi D, Saeidi M, Arji I. Effect of sucrose concentrations on Stevia rebaudiana Bertoni tissue culture and gene expression. Cell Mol Biol, 2017; 63(8): 32-36.
Kahrizi D, Ghari SM, Ghaheri M, Fallah F, Ghorbani T, Kazemi E, Ansarypour Z. Effect of KH2PO4 on gene expression, morphological and biochemical characteristics of stevia rebaudiana Bertoni under in vitro conditions. Cell Mol Biol, 2017; 63(7): 107-111.
Fallah F, Nokhasi F, Ghaheri M, Kahrizi D, Beheshti Ale Agha A, Ghorbani T, Kazemi E, Ansarypour Z. Effect of salinity on gene expression, morphological and biochemical characteristics of Stevia rebaudiana Bertoni under in vitro conditions. Cell Mol Biol, 2017; 63(7): 102-106.
Akbari F, Arminian A, Kahrizi D, Fazeli A. Effect of nitrogen sources on some morphological characteristics of in vitro stevia rebaudiana Bertoni. Cell Mol Biol, 2017;
(2): 107-111.
Hassanen SA, and Khalil RMA. Biotechnological Studies for Improving of Stevia (Stevia rebaudiana Bertoni) in vitro Plantlets Middle-East J Sci Res, 2013; 14: 93-106.
Lemus-Mondaca R, Vega-Gálvez A, Zura-Bravo L, Ah-Hen K. Stevia rebaudiana Bertoni, source of a high-potency natural sweetener: A comprehensive review on the biochemical, nutritional and functional aspects. Food Chem, 2012; 132: 1121–1132.
Reisa RV, Chierritoa TPC, Silva TFO, Albierob ALM, Souzac LA, Goncalves JE, Arildo JB, Oliveiraa AJB, Goncalves RAC. Morpho-anatomical study of Stevia rebaudiana roots grown in vitro and in vivo. Revista Brasil de Farmacog, 2017; 27 :34–39.
Hendawey MH, and Abo El Fadl RE. Biochemical studies on the production of active constituents in Stevia rebaudiana L. callus. Glob J Biotechnol Biochem, 2014; 9: 76-93.
Karimi R, Vahedi M, Pourmazaheri H, Khosro Balilashaki, KH. Biotechnological approaches in Stevia rebaudiana and its therapeutic applications. Adv Biomed Pharma, 2017; 4: 31-43.
Abd El-Motaleb M, Abd El-Hameid AS, Elnaggar HMN, Abdel-Hady M. Callus induction and regeneration of Stevia rebaudiana Bertoni. Int J Chem Tech Res, 2015; 8: 868-877.
Deo PC, Tyagi AP, Taylor M, Harding R, Becker D. Factors affecting somatic embryogenesis and transformation in modern plant breeding. South Pac Nat Appl Sci 2010; 28:27–40.
Nazneen H, Sridhar S, Reddy SK. studies on somatic embryogenesis of stevia rebaudiana (Bertoni) by scanning electron microscope (SEM). World J Pharmac Res; 2015; 4: 918-924.
Guruchandran V, and Sasikumar C. Organogenic plant regeneration via callus induction in Setevia rebaudiana Bert. Int J Curr Microbial App Sci, 2013; 2:56-61.
Singh, M.,, Saharan, V., Dayma, J., Rajpurohit, D., Yadunandan Sen, S and Ajay Sharma, A. (2017). In vitro Propagation of Stevia rebaudiana (Bertoni): An Overview, Review Article. Int.J.Curr.Microbiol.App.Sci. 6 :1010-1022.
Abdelmaksood AWM, Zavdetovna KL, Arnoldovna TO. Effect of different plant growth regulators on the in vitro induction and maintenance of callus from different explants of Hyoscyamus muticus L. J Appl Environ Biol Sci, 2017; 7:27-35.
Das K, Dang R, Rajasekharan PE. Establishment and maintenance of callus of stevia rebaudiana bertoni under aseptic environment. Ind J Natur Products and Resources. 5: 373–376.
Seabrook JEA, and Douglass L. Somatic embryogenesis on various potato tissues from a range of genotypes and ploidy levels. Plant Cell Rep, 2001; 20: 178-182.
Bi R, Kou MM, Chen LG, Mao SR, Wang HG. Plant regeneration through callus initiation from mature embryo of triticum. Plant Breed, 2007;.126:9-12.
Amali P, Kingsley SJ, Ignacimuthu S. High frequency callus induction and plant regeneration from shoot tip explants of sorghum bicolor. Int J Pharm and Pharmac Sci; 2014; 6: 213-216.

References

Esmaeili F, Kahrizi D, Mansouri M, Yari K., Kazemi, N, Ghaheri, M. Cell dedifferentiation in Stevia rebauiana as a pharmaceutical and medicinal plant. J Rep Pharmaceutic Sci, 2016; 5(1): 12-17.

Ghorbani T, Kahrizi D, Saeidi M, Arji I. Effect of sucrose concentrations on Stevia rebaudiana Bertoni tissue culture and gene expression. Cell Mol Biol, 2017; 63(8): 32-36.

Kahrizi D, Ghari SM, Ghaheri M, Fallah F, Ghorbani T, Kazemi E, Ansarypour Z. Effect of KH2PO4 on gene expression, morphological and biochemical characteristics of stevia rebaudiana Bertoni under in vitro conditions. Cell Mol Biol, 2017; 63(7): 107-111.

Fallah F, Nokhasi F, Ghaheri M, Kahrizi D, Beheshti Ale Agha A, Ghorbani T, Kazemi E, Ansarypour Z. Effect of salinity on gene expression, morphological and biochemical characteristics of Stevia rebaudiana Bertoni under in vitro conditions. Cell Mol Biol, 2017; 63(7): 102-106.

Akbari F, Arminian A, Kahrizi D, Fazeli A. Effect of nitrogen sources on some morphological characteristics of in vitro stevia rebaudiana Bertoni. Cell Mol Biol, 2017;

(2): 107-111.

Hassanen SA, and Khalil RMA. Biotechnological Studies for Improving of Stevia (Stevia rebaudiana Bertoni) in vitro Plantlets Middle-East J Sci Res, 2013; 14: 93-106.

Lemus-Mondaca R, Vega-Gálvez A, Zura-Bravo L, Ah-Hen K. Stevia rebaudiana Bertoni, source of a high-potency natural sweetener: A comprehensive review on the biochemical, nutritional and functional aspects. Food Chem, 2012; 132: 1121–1132.

Reisa RV, Chierritoa TPC, Silva TFO, Albierob ALM, Souzac LA, Goncalves JE, Arildo JB, Oliveiraa AJB, Goncalves RAC. Morpho-anatomical study of Stevia rebaudiana roots grown in vitro and in vivo. Revista Brasil de Farmacog, 2017; 27 :34–39.

Hendawey MH, and Abo El Fadl RE. Biochemical studies on the production of active constituents in Stevia rebaudiana L. callus. Glob J Biotechnol Biochem, 2014; 9: 76-93.

Karimi R, Vahedi M, Pourmazaheri H, Khosro Balilashaki, KH. Biotechnological approaches in Stevia rebaudiana and its therapeutic applications. Adv Biomed Pharma, 2017; 4: 31-43.

Abd El-Motaleb M, Abd El-Hameid AS, Elnaggar HMN, Abdel-Hady M. Callus induction and regeneration of Stevia rebaudiana Bertoni. Int J Chem Tech Res, 2015; 8: 868-877.

Deo PC, Tyagi AP, Taylor M, Harding R, Becker D. Factors affecting somatic embryogenesis and transformation in modern plant breeding. South Pac Nat Appl Sci 2010; 28:27–40.

Nazneen H, Sridhar S, Reddy SK. studies on somatic embryogenesis of stevia rebaudiana (Bertoni) by scanning electron microscope (SEM). World J Pharmac Res; 2015; 4: 918-924.

Guruchandran V, and Sasikumar C. Organogenic plant regeneration via callus induction in Setevia rebaudiana Bert. Int J Curr Microbial App Sci, 2013; 2:56-61.

Singh, M.,, Saharan, V., Dayma, J., Rajpurohit, D., Yadunandan Sen, S and Ajay Sharma, A. (2017). In vitro Propagation of Stevia rebaudiana (Bertoni): An Overview, Review Article. Int.J.Curr.Microbiol.App.Sci. 6 :1010-1022.

Abdelmaksood AWM, Zavdetovna KL, Arnoldovna TO. Effect of different plant growth regulators on the in vitro induction and maintenance of callus from different explants of Hyoscyamus muticus L. J Appl Environ Biol Sci, 2017; 7:27-35.

Das K, Dang R, Rajasekharan PE. Establishment and maintenance of callus of stevia rebaudiana bertoni under aseptic environment. Ind J Natur Products and Resources. 5: 373–376.

Seabrook JEA, and Douglass L. Somatic embryogenesis on various potato tissues from a range of genotypes and ploidy levels. Plant Cell Rep, 2001; 20: 178-182.

Bi R, Kou MM, Chen LG, Mao SR, Wang HG. Plant regeneration through callus initiation from mature embryo of triticum. Plant Breed, 2007;.126:9-12.

Amali P, Kingsley SJ, Ignacimuthu S. High frequency callus induction and plant regeneration from shoot tip explants of sorghum bicolor. Int J Pharm and Pharmac Sci; 2014; 6: 213-216.

Author biographies is not available.