Issue

Vol. 63 No. 11: Issue 11

The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.

Effect of melatonin on cytokine levels in a hyperthermia-induced febrile seizure model

https://doi.org/10.14715/cmb/2017.63.11.3
Leyla Aydin
Department of Physiology, Baskent University Medical Faculty, Ankara, Turkey
Erkan Yurtcu
Department of Medical Biology, Baskent University Medical Faculty, Ankara, Turkey
Yeşim Korkmaz
Department of Medical Biology, Baskent University Medical Faculty, Ankara, Turkey
Taner Sezer
Department of Pediatric Neurology, Baskent University Medical Faculty, Ankara, Turkey
Ersin Ogus
Department of Biostatistics, Baskent University Medical Faculty, Ankara, Turkey

Corresponding Author(s) : Leyla Aydin

leyla3b@yahoo.com

Cellular and Molecular Biology, Vol. 63 No. 11: Issue 11

Abstract

Higher serum cytokine levels have been reported in children admitted with febrile seizures and in some experimental models. However, other studies have shown that cytokine levels are influenced by melatonin. In this study, we investigated serum cytokine levels in a hyperthermia-induced febrile rat seizure model and the effect of melatonin. A total of 28 male Sprague–Dawley rats were divided into four groups: the control (C) group, healthy melatonin (MT) group, and hyperthermia-induced febrile seizure groups with (HIFS-MT) and without (HIFS) administration of melatonin. Melatonin (80 mg/kg) was given intraperitoneally 15 min before the seizure. HIFS was induced by placing the rats in 45°C water. The rats were sacrificed under anesthesia after the seizure. Blood samples were drawn by transcardiac puncture to measure serum cytokine and melatonin levels. Serum interleukin (IL)-1β, IL-6, IL-10, and tumor necrosis factor (TNF)-α levels were lower in the HIFS group than those in the C group (p = 0.005, p = 0.200, p = 0.011, and p = 0.016, respectively). All serum cytokine levels of rats in the MT and HIFS-MT groups were similar to those in the C group. This experimental rat model demonstrated that serum cytokine levels decrease with HIFS and that administering melatonin maintains serum cytokine levels. These results suggest that cytokines may play role in the anticonvulsive activity of melatonin in rats with febrile seizures.

Keywords

Hyperthermia-induced febrile seizures Anticonvulsive effect Melatonin Cytokine Serum.
Aydin, L., Yurtcu, E., Korkmaz, Y., Sezer, T., & Ogus, E. (2017). Effect of melatonin on cytokine levels in a hyperthermia-induced febrile seizure model. Cellular and Molecular Biology, 63(11), 11–16. https://doi.org/10.14715/cmb/2017.63.11.3
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. Stenklyft PH, Carmona M. Febrile seizures. Emerg Med Clin North Am 1994;12:989-99.
  2. Baram TZ, Shinnar S. Febrile seizures. In: The incidence and prevalence of febrile seizures. Stafstrom CE. San Diego: Academic Press; 2002. pp. 1–25.
  3. Ostergaard JR. Febrile seizures. Acta Paediatr 2009;98:771-3.
  4. Dubé CM, Ravizza T, Hamamura M, Zha Q, Keebaugh A, Fok K, et al. Epileptogenesis provoked by prolonged experimental febrile seizures: mechanisms and biomarkers. J Neurosci 2010:30(22):7484–94.
  5. Baulac S, Huberfeld G, Gourfinkel-An I, Mitropoulou G, Beranger A. Prud'homme JF, et al. First genetic evidence of GABA(A) receptor dysfunction in epilepsy: a mutation in the gamma2-subunit gene. Nat Genet 2001;28:46–48.
  6. Virta M, Hurme M, Helminen M. Increased plasma levels of pro- and anti-inflammatory cytokines in patients with febrile seizures. Epilepsia 2002;43(8):920-3.
  7. Turrin NP, Rivest S. Innate immune reaction in response to seizures: implications for the neuropathology associated with epilepsy. Neurobiol Dis 2004;16(2):321-34.
  8. Dube CM, Vezzani A, Behrens M, Bartfai T, Baram TZ. Interleukin-1b Contributes to the Generation of Experimental Febrile Seizures. Ann Neurol 2005:57:152–155.
  9. Ichiyama T, Suenaga N, Kajimoto M, Tohyama J, Isumi H, Kubota M, et al. Serum and CSF levels of cytokines in acute encephalopathy following prolonged febrile seizures. Brain and Development 2008;30:47-52.
  10. Sasaki K, Matsuo M, Maeda T, Zaitsu M, Hamasaki Y. Febrile seizures: characterization of double-stranded RNA-induced gene expression. Pediatr Neurol 2009;41(2):114-8.
  11. Choi J, Min HJ, Jeon-Soo Shin JS. Increased levels of HMGB1 and pro-inflammatory cytokines in children with febrile seizures. Journal of Neuroinflammation 2011;8:135-44.
  12. Gunawan A, Muid M, Suyudi H, Wisnu B, Subandiyah K. The Correlation Between IL-1β and IL-10 Levels in Estimating The Risk of Febrile Seizures. Journal of Tropical Life Science 2014;4(2):131-136.
  13. Guo JF, Yao BZ. Serum melatonin levels in children with epilepsy or febrile seizures. Zhongguo Dang Dai Er Ke Za Zhi 2009;11(4):288-90.
  14. Ardura J, Andres J, Garmendia JR, Ardura F. Melatonin in epilepsy and febrile seizures. J Child Neurol. 2010;25(7):888-91.
  15. Dabak O, Altun D, Arslan M, Yaman H, Vurucu S, Yesilkaya E, et al. Evaluation of Plasma Melatonin Levels in Children With Afebrile and Febrile Seizures. Pediatr Neurol. 2016;57:51-5.
  16. Peled N, Shorer Z, Peled E, Pillar G. Melatonin effect on seizures in children with severe neurologic deficit disorders. Epilepsia 2001;2(9):1208-10.
  17. Elkhayat HA, Hassanein SM, Tomoum HY, Abd-Elhamid IA, Asaad T, Elwakkad AS. Melatonin and sleep-related problems in children with intractable epilepsy. Pediatr Neurol. 2010;42(4):249-54.
  18. Borowicz KK, Kamiñski R, Gsior M, Kleinrok Z, Czuczwar SJ. Influence of melatonin upon the protective action of conventional anti-epileptic drugs against maximal electroshock in mice. Eur Neuropsychopharmacol 1999;9:185–190.
  19. Costa-Lotufo LV, de Fonteles MM, Lim ISP, de Oliveira AA, Nascimento VS, de Bruin VM, et al. Attenuating effects of melatonin on pilocarpine-induced seizures in rats. Comp Biochem Physiol C 2002,131:521–529.
  20. Yildirim M, Marangoz C. Anticonvulsant effects of melatonin on penicillin-induced epileptiform activity in rats. Brain Res 2006;1099:183-188.
  21. Yahyavi-Firouz-Abadi N, Tahsili-Fahadan P, Riazi K, Ghahremani MH, Dehpour AR. Melatonin enhances the anticonvulsant and proconvulsant effects of morphine in mice: Role for nitric oxide signaling pathway. Epilepsy Res 2007;75:138–144.
  22. Solmaz I, Gurkanlar D, Gokcil Z, Cuneyt G, Ozkan M, Erdogan E. Antiepileptic activity of melatonin in guinea pigs with pentylenetetrazol-induced seizures. Neurol Res 2009;31:989–995.
  23. Aydin L, Gundogan NU, Yazici C. Anticonvulsant efficacy of melatonin in an experimental model of hyperthermic febrile seizures. Epilepsy Res. 2015;118:49-54.
  24. Maestroni GJ. The photoperiod transducer melatonin and the immune hematopoietic system. J Photochem Photobiol B 1998;43:186-192.
  25. Rodrí­guez MI, Escames G, López LC, López A, Garcí­a JA, Ortiz F, et al. Chronic melatonin treatment reduces the age-dependent inflammatory process in senescence-accelerated mice. J Pineal Res 2007;42(3):272-9.
  26. Yuan X, Li B, Li H, Xiu R. Melatonin inhibits IL-1β-induced monolayer permeability of human umbilical vein endothelial cells via Rac activation. J Pineal Res 2011;51(2): 220-5.
  27. Moezi L, Shafaroodi H, Hojati A, Dehpour AR. The interaction of melatonin and agmatine on pentylenetetrazole-induced seizure threshold in mice. Epilepsy Behav 2011;22(2):200-6.
  28. Åotowska MES, Joanna M, Åotowska JM. The neuroprotective effect of topiramate on the ultrastructure of pyramidal neurons of the hippocampal CA1 and CA3 sectors in an experimental model of febrile seizures in rats. Folia Neuropathol 2011;49 (3):230-6.
  29. Jiang W, Duong TM, de Lanerolle NC. The neuropathology of hyperthermic seizures in the rat. Epilepsia 1999;40:5-19.
  30. Rossato LG, Costa VM, Dallegrave E, Arbo M, Dinis-Oliveira RJ, Santos-Silva A, et al. Cumulative mitoxantrone-induced haematological and hepatic adverse effects in a subchronic in vivo study. Basic Clin Pharmacol Toxicol 2014;114(3):254-62.
  31. Pervaiz N, Hoffman-Goetz L. Immune cell inflammatory cytokine responses differ between central and systemic compartments in response to acute exercise in mice. Exerc Immunol Rev 2012;18:142-57.
  32. Benot S, Goberna R, Reiter RJ, Garcia-Mauriño S, Osuna C, Guerrero JM. Physiological levels of melatonin contribute to the antioxidant capacity of human serum. J Pineal Res 1999;27(1):59-64.
  33. Heida JG, Pittman QJ. Causal links between brain cytokines and experimental febrile convulsions in the rat. Epilepsia 2005;46:1906-1913.
  34. Haveman J, Geerdink AG, Rodermond HM. Cytokine production after whole body and localized hyperthermia. Int J Hyperthermia 1996;12:791–800.
  35. Ostberg J R, Taylor SL, Baumann H, Repasky EA. Regulatory effects of fever-range whole-body hyperthermia on the LPS-induced acute inflammatory response. Journal of leukocyte biology 2000,68(6):815-820.
  36. Yamashita N, Hoshida S, Otsu K, Taniguchi N, Kuzuya T, Hori M. The involvement of cytokines in the second window of ischaemic preconditioning. Br J Pharmacol 2000;131(3):415-22.
  37. Nelson EA, Wong Y, Yu LM, Fok TF, Li K. Effects of hyperthermia and muramyl dipeptide on IL-1beta, IL-6, and mortality in a neonatal rat model. Pediatr Res 2002;52(6):886-91.
  38. Fairchild KD, Viscardi RM, Hester L, Singh IS, Hasday JD. Effects of hypothermia and hyperthermia on cytokine production by cultured human mononuclear phagocytes from adults and newborns. Journal of Interferon & Cytokine Research 2000;20(12):1049-1055.
  39. Zhao L, An R, Yang Y, Yang X, Liu H, Yue L, et al. Melatonin alleviates brain injury in mice subjected to cecal ligation and puncture via attenuating inflammation, apoptosis, and oxidative stress: the role of SIRT1 signaling. Journal of Pineal Research 2015;59(2):230-239.
  40. Ersoy OF, í–zkan N, í–zsoy Z, Kayaoğlu HA, Yenidoğan E, í‡elik A, et al. Effects of melatonin on cytokine release and healing of colonic anastomoses in an experimental sepsis model. Ulus Travma Acil Cerrahi Derg 2016;22(4):315-321.
  41. Carrillo-Vico A, Lardone PJ, Naji L, Fernández-Santos JM, Martí­n-Lacave I, Guerrero JM, et. al. Beneficial pleiotropic actions of melatonin in an experimental model of septic shock in mice: regulation of pro-/anti-inflammatory cytokine network, protection against oxidative damage and anti-apoptotic effects. J Pineal Res. 2005;39(4):400-8.
  42. Kahya MC, Naziroğlu M, í‡iğ B. Melatonin and selenium reduce plasma cytokine and brain oxidative stress levels in diabetic rats. Brain Inj. 2015;29(12):1490-6.
Read More
Author biographies is not available.
Crossref
Scopus
Google Scholar
Europe PMC
Download this PDF file
PDF
Statistic
Read Counter : 846 Download : 332