Baicalein induces apoptosis and reduces inflammation in LPS-stimulated keratinocytes by blocking the activation of NF-ÎºB: implications for alleviating oral lichen planus
Corresponding Author(s) : J Wang
Cellular and Molecular Biology,
Vol. 62 No. 7: Issue 7
ic inflammatory diseases, including OLP, involves in the activation of the nuclear factor-kappa B (NF-ÎºB) signaling pathway. Baicalein (BAI) is an alcohol soluble flavonoid known for its anti-inflammatory effect. However, its effectiveness on keratinocytes in OLP remains unclear. In the present study, we examined inflammation in oral mucosa tissue from OLP patients. Hematoxylin and eosin staining showed denser subepithelial lymphocytes infiltration compared to the normal oral mucosa epithelium. TNF-Î± and IL-6 were up-regulated in oral mucosa tissue of OLP patients. We next stimulated humans keratinocytes (HaCaT cells) with lipopolysaccharide (LPS) to create an inflammatory environment like that in the OLP tissue and assessed the effect of BAI on OLP and NF-ÎºB signaling pathways. Our results showed that BAI treatment inhibited the level of TNF-Î± and IL-6 induced by LPS. However, the cells apoptosis was promoted after BAI treatment. Furthermore, BAI not only inhibited LPS-induced p38 MAPK and ERK1/2 phosphorylation, but also NF-ÎºB activation by reducing IÎºBÎ± phosphorylation and the nuclear translocation of NFÎºB-p65 and NFÎºB-p50 from cytoplasm to nucleus in keratinocytes. Our findings suggest that BAI inhibits the production of inflammatory cytokines by negatively regulating the NF-ÎºB signaling pathway under LPS simulation in HaCaT cells.
Baicalein oral lichen planus NF-ÎºB apoptosis and inflammation.
Wang, J., Luo, H., Yang, L., & Li, Y. (2016). Baicalein induces apoptosis and reduces inflammation in LPS-stimulated keratinocytes by blocking the activation of NF-ÎºB: implications for alleviating oral lichen planus. Cellular and Molecular Biology, 62(7), 55–60. Retrieved from https://cellmolbiol.org/index.php/CMB/article/view/894
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